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[176314_at]

Description Y50D4B.6 /REP_DB=WormBase Gene ID /WP=CE26136 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 23      4grp_total > 60      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 99.4
(3)
P 		87.4
(1.5)
P 		95.8
(2.5)
P 		51.7
(-3)
A 		48
B 100.8
(1.4)
P 		117
(3)
P 		97.6
(1.1)
P 		55.6
(-3)
A 		61
C 113.4
(1.5)
P 		131.7
(3)
P 		116.5
(1.8)
P 		57.7
(-3)
A 		74
avr 104.5
(2.2) 		112
(3) 		103.3
(2.1) 		55
(-3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 84.4
(0.3)
P 		81
(0.1)
P 		147.1
(3)
P 		64.1
(-0.6)
A 		8.1
(-3)
A 		11.3
(-2.9)
A 		139
B 118.6
(3)
P 		74.5
(-0.1)
P 		60.1
(-1.1)
P 		46
(-2.1)
P 		33.4
(-3)
A 		34.8
(-2.9)
A 		85
C 74.1
(1.1)
P 		98.7
(3)
P 		72.9
(1)
A 		67.6
(0.5)
P 		42.3
(-1.5)
A 		22.7
(-3)
A 		76
avr 92.4
(2.9) 		84.7
(2.3) 		93.4
(3) 		59.2
(0.1) 		27.9
(-2.6) 		22.9
(-3) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 36.2
(3)
P 		19
(-0.6)
P 		25.1
(0.7)
P 		7.6
(-3)
A 		29
B 32.2
(3)
P 		28.9
(2)
P 		22.6
(0.2)
P 		11.9
(-3)
A 		20
C 37.5
(2.4)
P 		37.9
(2.5)
P 		40.6
(3)
P 		9.7
(-3)
A 		31
avr 35.3
(3) 		28.6
(1.4) 		29.4
(1.6) 		9.7
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 15.6
(-0.3)
P 		28.8
(2.4)
P 		32
(3)
P 		16.2
(-0.2)
A 		2.4
(-3)
A 		2.8
(-2.9)
A 		30
B 41.3
(3)
P 		24.8
(-0.4)
P 		14.6
(-2.5)
P 		12.2
(-3)
P 		13.6
(-2.7)
A 		13
(-2.8)
A 		29
C 27.5
(2.3)
P 		30.1
(3)
P 		18.5
(-0.1)
A 		13.6
(-1.4)
P 		13.7
(-1.4)
A 		7.6
(-3)
A 		23
avr 28.1
(3) 		27.9
(2.9) 		21.7
(1.1) 		14
(-1.2) 		9.9
(-2.4) 		7.8
(-3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176314_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15216
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.6 NCBI
Target Description Y50D4B.6 /REP_DB=WormBase Gene ID /WP=CE26136 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070974(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070974 NCBI Caenorhabditis elegans Y50D4B.6 (Y50D4B.6) mRNA, complete cds. 11/11 None
SNAP00000024546 ENSEMBL cdna:SNAP chromosome:CEL140:V:1084214:1086950:1 11/11 None
GENEFINDER00000024553 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1084214:1087032:1 11/11 None
Y50D4B.6 ENSEMBL cdna:known chromosome:CEL140:V:1084191:1087102:1 gene:Y50D4B.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1083950-1086950(+) 91.61 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4B.6
Entrez Gene 3565593 Entrez gene
SwissProt Q9N4T2 EMBL-EBI
WormBase CE37685 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF59493 Hypothetical protein Y50D4B.6 [Caenorhabditis elegans] ref|NP_503375.2| Y50D4B.6 [Caenorhabditis elegans] 0.0
blast CAC42353 Hypothetical protein W04G5.10 [Caenorhabditis elegans] ref|NP_492969.1| W04G5.10 [Caenorhabditis elegans] 1.0E-84
blast CAC42353 Hypothetical protein W04G5.10 [Caenorhabditis elegans] ref|NP_492969.1| W04G5.10 [Caenorhabditis elegans] 3.0E-84
blast CAA91146 Hypothetical protein C08H9.5 [Caenorhabditis elegans] ref|NP_496130.1| Overexpression Longevity Determinant family member (old-1) [Caenorhabditis elegans] 2.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.9E-53
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503375.2 1 7-26
Y50D4B.6 1 7-26
SNAP00000024546 1 7-26
GENEFINDER00000024553 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:176314_AT
agataatggtcgtttgctgtgcccgacggacttgttatcatttgcttttcagattgctaa
tggaatgaagtttttaggcaatgtcgcgtgtgttcatcgcgatctggcactacgaaatgt
gtttgtcaaaagaaatcgaattattcgaattgctgattttggattggctagatggcatga
aaacaaagaatattatagaaagaagaccgacgtaggttttccaatgaagtggttggcacc
ggagtgtttcgagtttgaacaagaacgcgagaatataaagtttgattcgaaatctgacat
ttggtcatatggagtttgtctttatgaaatattttcattgggagtgtcaccttatgaggg
tctcgattttcggccagattatttccagggtttgatgaagtacgtgagagatgggaatca
acttccaagtccggagcatgggagtgataaaatctacgaattcatgcagtcttgctggaa
cttaaatccggataaacgacccgtcttctcagaatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATAATGGTCGTTTGCTGTGCCCG 681 65 663 Antisense
GCCCGACGGACTTGTTATCATTTGC 410 285 683 Antisense
AGGCAATGTCGCGTGTGTTCATCGC 143 55 738 Antisense
TTCATCGCGATCTGGCACTACGAAA 39 679 755 Antisense
GGCACCGGAGTGTTTCGAGTTTGAA 237 533 897 Antisense
GGGAGTGTCACCTTATGAGGGTCTC 19 497 1002 Antisense
TATGAGGGTCTCGATTTTCGGCCAG 629 655 1015 Antisense
TCGGCCAGATTATTTCCAGGGTTTG 437 603 1032 Antisense
GGAATCAACTTCCAAGTCCGGAGCA 621 531 1076 Antisense
ATCTACGAATTCATGCAGTCTTGCT 590 31 1114 Antisense
TAAACGACCCGTCTTCTCAGAATGC 3 639 1155 Antisense
Sequence Source Affymetrix Proprietary Database