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[176313_at]

Description F42H10.3 /REP_DB=WormBase Gene ID /WP=CE00165 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 08      4grp_high_8_8 > 52      6grp_highest_nonorm > 52      6grp_total > 41      4grp_total > 51      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 606
1 2 3 4 range
A 556.2
(0.7)
P 		758.7
(3)
P 		641.1
(1.7)
P 		232.6
(-3)
P 		526
B 474.2
(0.1)
P 		771.9
(3)
P 		640.1
(1.7)
P 		165.9
(-3)
P 		606
C 606.2
(1)
P 		769
(3)
P 		656.9
(1.6)
P 		272.7
(-3)
P 		496
avr 545.5
(0.6) 		766.5
(3) 		646
(1.7) 		223.7
(-3) 		543

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 932
1 2 3 4 5 6 range
A 715.3
(1.1)
P 		870.7
(2.7)
P 		903.1
(3)
P 		698.2
(1)
P 		495
(-1.1)
P 		299.3
(-3)
P 		604
B 667.1
(-0.5)
P 		807.5
(0.4)
P 		1205.2
(3)
P 		827.2
(0.6)
P 		522
(-1.4)
P 		273.5
(-3)
P 		932
C 858.1
(2.4)
P 		803.9
(1.9)
P 		916.6
(3)
P 		855.5
(2.4)
P 		483.9
(-1.4)
P 		322.8
(-3)
P 		594
avr 746.8
(0.8) 		827.4
(1.5) 		1008.3
(3) 		793.6
(1.2) 		500.3
(-1.3) 		298.5
(-3) 		710
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 202.5
(3)
P 		164.7
(1.7)
P 		167.6
(1.8)
P 		34.1
(-3)
P 		168
B 151.2
(1.5)
P 		190.6
(3)
P 		148.3
(1.4)
P 		35.4
(-3)
P 		155
C 200.7
(2.1)
P 		221.1
(2.7)
P 		228.9
(3)
P 		46
(-3)
P 		183
avr 184.8
(2.7) 		192.1
(3) 		181.6
(2.6) 		38.5
(-3) 		154

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 131.9
(-1.5)
P 		309.7
(3)
P 		196.7
(0.1)
P 		177.1
(-0.4)
P 		149.4
(-1.1)
P 		72.9
(-3)
P 		237
B 232.1
(1.1)
P 		268.8
(2.3)
P 		292.6
(3)
P 		219.1
(0.7)
P 		212.1
(0.5)
P 		102
(-3)
P 		191
C 318.1
(3)
P 		245.3
(0.9)
P 		233.3
(0.6)
P 		172
(-1.2)
P 		156.6
(-1.6)
P 		108.1
(-3)
P 		210
avr 227.4
(1.4) 		274.6
(3) 		240.9
(1.9) 		189.4
(0.2) 		172.7
(-0.4) 		94.3
(-3) 		180
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176313_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6991
Sequence Type Exemplar sequence
Representative Public ID F42H10.3 NCBI
Target Description F42H10.3 /REP_DB=WormBase Gene ID /WP=CE00165 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066473(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066473 NCBI Caenorhabditis elegans F42H10.3 (F42H10.3) mRNA, complete cds. 10/11 None
SNAP00000027854 ENSEMBL cdna:SNAP chromosome:CEL140:III:8486565:8488030:1 10/11 None
GENEFINDER00000027865 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8486565:8488030:1 10/11 None
F42H10.3 ENSEMBL cdna:known chromosome:CEL140:III:8484066:8488030:1 gene:F42H10.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8486559-8488025(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F42H10.3
Entrez Gene 185685 Entrez gene
SwissProt P34416 EMBL-EBI
WormBase CE37108 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSGENOME1:148909_AT dm
DROSOPHILA_2:1637874_AT dm
Protein Similarities
Method ID Description E-Value
blast AAT92076 Hypothetical protein F42H10.3 [Caenorhabditis elegans] ref|NP_498874.2| F42H10.3 [Caenorhabditis elegans] sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3 1.0E-163
blast AAT92076 Hypothetical protein F42H10.3 [Caenorhabditis elegans] ref|NP_498874.2| F42H10.3 [Caenorhabditis elegans] sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3 1.0E-78
blast CAE71248 Hypothetical protein CBG18124 [Caenorhabditis briggsae] 4.0E-75
blast CAE71247 Hypothetical protein CBG18123 [Caenorhabditis briggsae] 4.0E-74
blast CAE71247 Hypothetical protein CBG18123 [Caenorhabditis briggsae] 3.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR001452 EMBL-EBI SH3 3.3E-22
Pfam IPR001452 EMBL-EBI SH3 3.3E-22
Pfam IPR000900 EMBL-EBI Nebulin 5.2E-11
Pfam IPR000900 EMBL-EBI Nebulin 1.0E-4
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 3.4E-13
Sequence
Target Sequence 
>C. ELEGANS:176313_AT
gaaccatcaatcatattcggctccaacacaagcagtagccgcaaatacgcatttgatcta
ctcaacagagcaaggtggagcagtatctccaacaccacaaaaatcaatcggaagtattgc
tgactatgatccaatgaacggacaatggggtacagctgctgctcaaccgagaaattccga
gaaacttggatatttgagtaaggattttgtttttttttttggcttttgtttgaatgattt
gctcatttgtaatccacacaaattttttgcacgttttgaggctcaagtggataaaggtcc
ggcaagattttgtgcggattttgcaggagctccacctccatcatcaaatagtatatcttc
aacatctccacattcaacactttcttctccacaaagcacaatttcaccgactggaaaagc
tggatttgcagttaaggcaatttatgattacgctgctgctgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCATCAATCATATTCGGCTCCA 683 345 15 Antisense
ATTCGGCTCCAACACAAGCAGTAGC 437 5 29 Antisense
AAGCAGTAGCCGCAAATACGCATTT 509 149 44 Antisense
CAGTATCTCCAACACCACAAAAATC 333 207 95 Antisense
GACAATGGGGTACAGCTGCTGCTCA 667 363 155 Antisense
GATTTGCTCATTTGTAATCCACACA 43 429 250 Antisense
TGTGCGGATTTTGCAGGAGCTCCAC 9 557 325 Antisense
CTCCACCTCCATCATCAAATAGTAT 153 237 344 Antisense
ACACTTTCTTCTCCACAAAGCACAA 333 109 391 Antisense
ACAAAGCACAATTTCACCGACTGGA 568 111 405 Antisense
ATTTATGATTACGCTGCTGCTGATA 495 15 454 Antisense
Sequence Source Affymetrix Proprietary Database