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[176312_at]

Description Y50D4B.7 /REP_DB=WormBase Gene ID /WP=CE26137 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 10      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 17.9
(3)
A 		10.9
(0.1)
A 		3.6
(-3)
A 		5.2
(-2.3)
A 		14
B 6.6
(-0.6)
A 		9
(3)
A 		5
(-3)
A 		5.3
(-2.6)
A 		4
C 4
(-3)
A 		11.4
(-2)
A 		18.2
(-1.1)
A 		48.3
(3)
A 		44
avr 9.5
(-2.7) 		10.4
(-2.2) 		8.9
(-3) 		19.6
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 12.1
(3)
A 		6.3
(-0.3)
A 		2.2
(-2.6)
A 		2
(-2.7)
A 		1.4
(-3)
A 		1.8
(-2.8)
A 		11
B 17.1
(3)
A 		0.9
(-3)
A 		14.1
(1.9)
A 		16.2
(2.7)
A 		6.7
(-0.9)
A 		0.9
(-3)
A 		16
C 10.1
(-1.6)
A 		2.4
(-3)
A 		3.3
(-2.8)
A 		34.3
(3)
A 		9.7
(-1.6)
P 		2.8
(-2.9)
A 		32
avr 13.1
(1.3) 		3.2
(-2.5) 		6.5
(-1.2) 		17.5
(3) 		5.9
(-1.4) 		1.8
(-3) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 6.5
(3)
A 		2.4
(-1.3)
A 		0.9
(-2.9)
A 		0.8
(-3)
A 		6
B 2.1
(2.5)
A 		2.2
(3)
A 		1.2
(-2.5)
A 		1.1
(-3)
A 		1
C 1.3
(-3)
A 		3.3
(-1.2)
A 		6.3
(1.4)
A 		8.1
(3)
A 		7
avr 3.3
(3) 		2.6
(-3) 		2.8
(-1.3) 		3.3
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 2.2
(3)
A 		2.2
(3)
A 		0.5
(-2.7)
A 		0.5
(-2.7)
A 		0.4
(-3)
A 		0.4
(-3)
A 		2
B 5.9
(3)
A 		0.3
(-3)
A 		3.4
(0.3)
A 		4.3
(1.3)
A 		2.7
(-0.4)
A 		0.3
(-3)
A 		6
C 3.7
(-0.1)
A 		0.7
(-3)
A 		0.8
(-2.9)
A 		6.9
(3)
A 		3.1
(-0.7)
P 		0.9
(-2.8)
A 		6
avr 3.9
(3) 		1.1
(-1.9) 		1.6
(-1.1) 		3.9
(3) 		2.1
(-0.2) 		0.5
(-3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176312_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15104
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.7 NCBI
Target Description Y50D4B.7 /REP_DB=WormBase Gene ID /WP=CE26137 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070973(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070973 NCBI Caenorhabditis elegans Y50D4B.7 (Y50D4B.7) mRNA, complete cds. 11/11 None
GENEFINDER00000024554 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1080784:1083557:-1 9/11 None
Y50D4B.7 ENSEMBL cdna:known chromosome:CEL140:V:1081451:1083777:-1 gene:Y50D4B.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000024545 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1081450-1083777(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4B.7
Entrez Gene 190097 Entrez gene
SwissProt Q9N4T3 EMBL-EBI
WormBase CE26137 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59492 Hypothetical protein Y50D4B.7 [Caenorhabditis elegans] ref|NP_503374.1| Y50D4B.7 [Caenorhabditis elegans] 0.0
blast AAF59492 Hypothetical protein Y50D4B.7 [Caenorhabditis elegans] ref|NP_503374.1| Y50D4B.7 [Caenorhabditis elegans] 1.0E-154
blast AAC27091 Hypothetical protein C04E12.5 [Caenorhabditis elegans] ref|NP_503935.2| C04E12.5 [Caenorhabditis elegans] 2.0E-66
blast AAC27089 Hypothetical protein C04E12.4 [Caenorhabditis elegans] ref|NP_503936.2| C04E12.4 [Caenorhabditis elegans] 4.0E-61
blast AAC27091 Hypothetical protein C04E12.5 [Caenorhabditis elegans] ref|NP_503935.2| C04E12.5 [Caenorhabditis elegans] 4.0E-54
blast AAC27089 Hypothetical protein C04E12.4 [Caenorhabditis elegans] ref|NP_503936.2| C04E12.4 [Caenorhabditis elegans] 5.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 3.5E-39
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.0E-43
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.0E-43
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503374.1 1 24-46
Y50D4B.7 1 24-46
Sequence
Target Sequence 
>C. ELEGANS:176312_AT
ggggtgtgtcagatcagtattttttatctatatacttctattctccaagcgaaattattc
cattacatccggatttggaagataactacgtgaaatttacatatgacattatcagcaaca
cttattcacacaaacgacaaaatggatcatcacttgaaccgtatttcgttggtaatgtaa
aacgagtagaaaataggacattgggtgtcgtttattttgaaaaaatcgataaaacaatta
ttggaacaattctctgggagttcggattaaaggttgttgggaaatcaattgaaaaaatag
taattaaaatgaatggaatgcaagaaattgatgattatggagaaagtgccgcagcttgtc
aaccttctttgaataaatgcactgagatccaaataaatgacagtttgactattgaaaagc
ccagtgatcaaattgctatatctataagtatgaacgaggatataaaagttttaaaagcag
aactaaaggaaggtaacaaagtggaaagtttctcagttgaagtctactggggtgagttaa
tttttattttgagctttaaactgacggcggtcaaagtttttcaagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGGTGTGTCAGATCAGTATTTTTT 88 499 698 Antisense
TATATACTTCTATTCTCCAAGCGAA 514 661 726 Antisense
AGCGAAATTATTCCATTACATCCGG 57 83 745 Antisense
ATCATCACTTGAACCGTATTTCGTT 616 29 843 Antisense
AACAATTCTCTGGGAGTTCGGATTA 233 135 942 Antisense
GAGAAAGTGCCGCAGCTTGTCAACC 29 393 1037 Antisense
AGCTTGTCAACCTTCTTTGAATAAA 77 79 1050 Antisense
AAAGCCCAGTGATCAAATTGCTATA 109 125 1113 Antisense
GAAAGTTTCTCAGTTGAAGTCTACT 170 361 1201 Antisense
TTTGAGCTTTAAACTGACGGCGGTC 260 659 1245 Antisense
GACGGCGGTCAAAGTTTTTCAAGCT 96 377 1260 Antisense
Sequence Source Affymetrix Proprietary Database