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[176307_at]

Description Y22D7AR.F /REP_DB=WormBase Gene ID /WP=CE26512 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 54      4grp_total > 40      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 1.4
(-3)
A 		3.1
(1.3)
A 		3.8
(3)
A 		3.1
(1.3)
A 		2
B 2.1
(-2.9)
A 		1.8
(-3)
A 		15
(3)
A 		1.9
(-3)
A 		13
C 1.8
(-2)
A 		1.3
(-3)
A 		2.8
(-0)
A 		4.3
(3)
A 		3
avr 1.8
(-3) 		2.1
(-2.7) 		7.2
(3) 		3.1
(-1.6) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 4.2
(3)
A 		2.9
(0.5)
A 		1.2
(-2.8)
A 		1.8
(-1.6)
A 		1.1
(-3)
A 		3.8
(2.2)
A 		3
B 3.2
(-1.8)
A 		1.2
(-3)
A 		3.9
(-1.4)
A 		3
(-1.9)
A 		1.3
(-2.9)
A 		11.4
(3)
A 		10
C 4.4
(-2)
A 		1.8
(-2.7)
A 		22.4
(3)
A 		2.2
(-2.6)
A 		1.6
(-2.7)
A 		0.6
(-3)
A 		22
avr 3.9
(-1) 		2
(-2.5) 		9.2
(3) 		2.3
(-2.2) 		1.3
(-3) 		5.3
(0) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 0.5
(-3)
A 		0.7
(-0.6)
A 		1
(3)
A 		0.5
(-3)
A 		1
B 0.7
(-2.4)
A 		0.4
(-3)
A 		3.5
(3)
A 		0.4
(-3)
A 		3
C 0.6
(-1)
A 		0.4
(-3)
A 		1
(3)
A 		0.7
(-0)
A 		1
avr 0.6
(-2.5) 		0.5
(-3) 		1.8
(3) 		0.5
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 0.8
(1.3)
A 		1
(3)
A 		0.3
(-3)
A 		0.4
(-2.1)
A 		0.3
(-3)
A 		0.9
(2.1)
A 		1
B 1.1
(-1.9)
A 		0.4
(-3)
A 		1
(-2.1)
A 		0.8
(-2.4)
A 		0.5
(-2.8)
A 		4.2
(3)
A 		4
C 1.6
(-1.5)
A 		0.5
(-2.7)
A 		5.7
(3)
A 		0.4
(-2.8)
A 		0.5
(-2.7)
A 		0.2
(-3)
A 		6
avr 1.2
(-0.5) 		0.6
(-2.4) 		2.3
(3) 		0.5
(-2.7) 		0.4
(-3) 		1.8
(1.4) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176307_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19067
Sequence Type Exemplar sequence
Representative Public ID Y22D7AR.F NCBI
Target Description Y22D7AR.F /REP_DB=WormBase Gene ID /WP=CE26512 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027655(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027655 NCBI Caenorhabditis elegans Y22D7AR.14 (Y22D7AR.14) mRNA, complete cds. 11/11 None
GENEFINDER00000025843 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1682785:1693060:-1 11/11 None
SNAP00000025833 ENSEMBL cdna:SNAP chromosome:CEL140:III:1688133:1698909:-1 11/11 None
Y22D7AR.14 ENSEMBL cdna:known chromosome:CEL140:III:1688133:1691759:-1 gene:Y22D7AR.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1688132-1692874(-) 92.27 100.0
Public Domain and Genome References
Gene Title
Ensembl Y22D7AR.14
Entrez Gene 3565517 Entrez gene
SwissProt Q86MI8 EMBL-EBI
WormBase CE33724 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO91683 Hypothetical protein Y22D7AR.14 [Caenorhabditis elegans] ref|NP_001022826.1| Y22D7AR.14 [Caenorhabditis elegans] 0.0
blast AAK29868 Hypothetical protein Y22D7AR.7 [Caenorhabditis elegans] ref|NP_497444.1| Y22D7AR.7 [Caenorhabditis elegans] 0.0
blast AAO91683 Hypothetical protein Y22D7AR.14 [Caenorhabditis elegans] ref|NP_001022826.1| Y22D7AR.14 [Caenorhabditis elegans] 1.0E-177
blast AAO91685 Hypothetical protein Y22D7AR.9 [Caenorhabditis elegans] ref|NP_497446.3| Y22D7AR.9 [Caenorhabditis elegans] 1.0E-168
blast AAO91683 Hypothetical protein Y22D7AR.14 [Caenorhabditis elegans] ref|NP_001022826.1| Y22D7AR.14 [Caenorhabditis elegans] 1.0E-163
blast AAO91684 Serpentine receptor, class d (delta) protein 64 [Caenorhabditis elegans] ref|NP_497445.2| Serpentine Receptor, class D (delta) family member (srd-64) [Caenorhabditis elegans] 1.0E-117
blast AAC68927 Hypothetical protein T12B5.1 [Caenorhabditis elegans] ref|NP_497307.1| T12B5.1 [Caenorhabditis elegans] 3.0E-34
blast AAK29873 Hypothetical protein Y22D7AR.2 [Caenorhabditis elegans] ref|NP_497449.1| Y22D7AR.2 [Caenorhabditis elegans] 2.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 6.7E-18
Pfam IPR000242 EMBL-EBI Tyrosine specific protein phosphatase 2.4E-35
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 1.8E-17
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.7E-38
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 4.3E-12
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 6.7E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022826.1 1 13-31
Y22D7AR.14 1 13-31
GENEFINDER00000025843 6 66-85,120-142,174-196,201-223,304-326,985-1007
Sequence
Target Sequence 
>C. ELEGANS:176307_AT
gaatgctacattacacagcttggaaggatatcgtaaagaattgaaagatttagaaagctc
tggtgttgactggacccaactgggcatgtttattcggactctgttcggattcgcagactt
tgtggaacttcttcatgcagggcatgataactggataaatgtaaatgaaactgctttgga
tatcgaaaaattgtcaaccttcaatttgtctatgtatgaaggtcttccaaaaagacctga
aattgaaaacggtttgaaatttttcccaattctaaaaaaatcagttgaagtaactgaaga
gtatgcctcaatacctttctataatgcgtctaaaaatgagccggcaattgtagaacttat
tgcaaacttgaagcttttcaaggaggttgcggaaaatttgaataatatttttgggcagac
acatgataatttcaaatattcaacccatattgtcggattaatcaatggatatattgattt
aataaaaattcatgatgatttgacggaagaatctattcatcctgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGCTACATTACACAGCTTGGAA 249 331 1152 Antisense
AAAGCTCTGGTGTTGACTGGACCCA 302 123 1205 Antisense
GACTGGACCCAACTGGGCATGTTTA 363 373 1219 Antisense
GGCATGTTTATTCGGACTCTGTTCG 272 537 1234 Antisense
GACTCTGTTCGGATTCGCAGACTTT 49 371 1248 Antisense
TGTGGAACTTCTTCATGCAGGGCAT 148 555 1272 Antisense
TTGTCAACCTTCAATTTGTCTATGT 359 705 1342 Antisense
GAGTATGCCTCAATACCTTTCTATA 481 399 1450 Antisense
ATGAGCCGGCAATTGTAGAACTTAT 93 45 1487 Antisense
TATTCAACCCATATTGTCGGATTAA 630 661 1588 Antisense
GACGGAAGAATCTATTCATCCTGTA 199 377 1653 Antisense
Sequence Source Affymetrix Proprietary Database