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[176305_at]

Description Y22D7AR.D /REP_DB=WormBase Gene ID /WP=CE23412 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 90      6grp_total > 40      4grp_total > 21      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 168.5
(-0.2)
P 		148.1
(-3)
P 		191.6
(3)
P 		185.9
(2.2)
P 		44
B 177.1
(1.4)
P 		154.6
(-0.5)
P 		124.9
(-3)
P 		195.5
(3)
P 		71
C 221.5
(3)
P 		172.8
(-0.1)
P 		126.9
(-3)
P 		220.9
(3)
P 		95
avr 189
(1.7) 		158.5
(-1.8) 		147.8
(-3) 		200.8
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 167.4
(-0.3)
P 		116.5
(-2.5)
P 		240.9
(3)
P 		188.7
(0.7)
P 		105.9
(-3)
P 		119.9
(-2.4)
P 		135
B 141.3
(-0.4)
P 		196.8
(3)
P 		192.5
(2.7)
P 		141.9
(-0.4)
P 		98.7
(-3)
P 		133.4
(-0.9)
P 		98
C 127.7
(-0.2)
P 		141.1
(0.4)
P 		161.9
(1.3)
P 		200.3
(3)
P 		64.5
(-3)
P 		123.8
(-0.4)
P 		136
avr 145.5
(0.1) 		151.5
(0.4) 		198.4
(3) 		177
(1.8) 		89.7
(-3) 		125.7
(-1) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 61.4
(3)
P 		32.1
(-2.1)
P 		50.1
(1)
P 		27.2
(-3)
P 		34
B 56.5
(3)
P 		38.2
(-1)
P 		28.9
(-3)
P 		41.7
(-0.2)
P 		28
C 73.3
(3)
P 		49.7
(-0.9)
P 		44.2
(-1.9)
P 		37.3
(-3)
P 		36
avr 63.7
(3) 		40
(-2) 		41.1
(-1.8) 		35.4
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 30.9
(-2.6)
P 		41.4
(0.1)
P 		52.5
(3)
P 		47.9
(1.8)
P 		32
(-2.3)
P 		29.2
(-3)
P 		23
B 49.2
(-0.5)
P 		65.5
(3)
P 		46.8
(-1)
P 		37.6
(-3)
P 		40.1
(-2.5)
P 		49.7
(-0.4)
P 		28
C 47.3
(3)
P 		43.1
(2)
P 		41.2
(1.6)
P 		40.3
(1.4)
P 		20.9
(-3)
P 		41.5
(1.7)
P 		26
avr 42.5
(0.6) 		50
(3) 		46.8
(2) 		41.9
(0.4) 		31
(-3) 		40.1
(-0.1) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176305_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19071
Sequence Type Exemplar sequence
Representative Public ID Y22D7AR.D NCBI
Target Description Y22D7AR.D /REP_DB=WormBase Gene ID /WP=CE23412 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065047(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065047 NCBI Caenorhabditis elegans Y22D7AR.11 (Y22D7AR.11) mRNA, complete cds. 11/11 None
SNAP00000025834 ENSEMBL cdna:SNAP chromosome:CEL140:III:1701767:1707557:-1 11/11 None
GENEFINDER00000025848 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1701778:1705475:-1 11/11 None
Y22D7AR.11 ENSEMBL cdna:known chromosome:CEL140:III:1703536:1705475:-1 gene:Y22D7AR.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1703535-1705475(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y22D7AR.11
Entrez Gene 189517 Entrez gene
SwissProt Q9BKX3 EMBL-EBI
WormBase CE23412 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29865 Hypothetical protein Y22D7AR.11 [Caenorhabditis elegans] ref|NP_497448.1| Y22D7AR.11 [Caenorhabditis elegans] 1.0E-157
blast AAK29865 Hypothetical protein Y22D7AR.11 [Caenorhabditis elegans] ref|NP_497448.1| Y22D7AR.11 [Caenorhabditis elegans] 1.0E-156
blast AAK29865 Hypothetical protein Y22D7AR.11 [Caenorhabditis elegans] ref|NP_497448.1| Y22D7AR.11 [Caenorhabditis elegans] 1.0E-135
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 9.0E-52
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 1.0E-51
blast CAB03489 Hypothetical protein ZC47.4 [Caenorhabditis elegans] ref|NP_497367.1| ZC47.4 [Caenorhabditis elegans] 2.0E-47
blast NP_503905 T28A11.21 [Caenorhabditis elegans] gb|AAC48129.2| Hypothetical protein T28A11.21 [Caenorhabditis elegans] 1.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 9.3E-61
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 9.3E-61
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.7E-14
Sequence
Target Sequence 
>C. ELEGANS:176305_AT
acacagaatatacacctgcccacgtgaaatcttttttagaaattttaaaattgaaaaaaa
gggtcaacgtgaagaaaattgacctcaaaaatttcctattcactgatattttatcgatta
tttcttatatgaatgctcaagtgatagaatatataaaaatgcaatatgttggctcagtcg
atcaatttgaacgtctcacttgtctggatcagtggaaaaatgcacattattgtatcattg
tggattccgagctggaaaatgtgccaattgaagattttttccactttaaagagtttgaag
ttgaatctagaagcttttcaacacaaaatgcgattgagatccgaaatgatctcttgaaaa
gaagctcattccaaagttgcacaatatggttccatggcggcaattcaaatgaaattgcaa
aagtcttccaaccggattatgatggtgacaatgaatatgagcttgattatattaccggtg
acaatgccaaatttgagattcgatgcggtattggcttattcgttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACAGAATATACACCTGCCCACGT 56 111 299 Antisense
CCTGCCCACGTGAAATCTTTTTTAG 372 267 313 Antisense
TGCAATATGTTGGCTCAGTCGATCA 81 577 458 Antisense
CAGTCGATCAATTTGAACGTCTCAC 685 207 473 Antisense
TGAACGTCTCACTTGTCTGGATCAG 96 575 486 Antisense
GTATCATTGTGGATTCCGAGCTGGA 47 455 530 Antisense
ATATGGTTCCATGGCGGCAATTCAA 234 21 682 Antisense
ATTGCAAAAGTCTTCCAACCGGATT 157 3 712 Antisense
TGAGCTTGATTATATTACCGGTGAC 598 569 756 Antisense
TACCGGTGACAATGCCAAATTTGAG 469 647 771 Antisense
GATGCGGTATTGGCTTATTCGTTGA 164 413 800 Antisense
Sequence Source Affymetrix Proprietary Database