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[176287_at]

Description Y50D4B.1 /REP_DB=WormBase Gene ID /WP=CE26132 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 47      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 49.3
(3)
A 		9.7
(-2.9)
A 		8.7
(-3)
A 		17.1
(-1.8)
A 		41
B 11.7
(-0.8)
A 		14.5
(2.4)
A 		9.8
(-3)
A 		15
(3)
A 		5
C 54.8
(3)
A 		16.5
(-2.2)
A 		14
(-2.6)
A 		11
(-3)
A 		44
avr 38.6
(3) 		13.6
(-2.4) 		10.8
(-3) 		14.4
(-2.2) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 15.5
(2.6)
A 		16.1
(3)
A 		12
(0.4)
A 		6.8
(-2.9)
A 		6.6
(-3)
A 		8.4
(-1.9)
A 		10
B 56.4
(3)
A 		11.9
(-2.4)
A 		7.1
(-3)
A 		16.8
(-1.8)
A 		10.5
(-2.6)
A 		9
(-2.8)
A 		49
C 32.6
(3)
A 		20.3
(0.1)
A 		27.2
(1.7)
A 		11.8
(-1.8)
A 		9.4
(-2.4)
A 		6.8
(-3)
A 		26
avr 34.8
(3) 		16.1
(-1.2) 		15.4
(-1.4) 		11.8
(-2.2) 		8.8
(-2.8) 		8.1
(-3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 18
(3)
A 		2.1
(-3)
A 		2.3
(-2.9)
A 		2.5
(-2.8)
A 		16
B 3.7
(3)
A 		3.6
(2.6)
A 		2.3
(-3)
A 		3.2
(0.9)
A 		1
C 18.1
(3)
A 		4.8
(-1.9)
A 		4.9
(-1.9)
A 		1.9
(-3)
A 		16
avr 13.3
(3) 		3.5
(-2.4) 		3.2
(-2.6) 		2.5
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 2.9
(-1.2)
A 		5.7
(3)
A 		2.6
(-1.7)
A 		1.7
(-3)
A 		2
(-2.6)
A 		2.1
(-2.4)
A 		4
B 19.6
(3)
A 		4
(-2.2)
A 		1.7
(-3)
A 		4.5
(-2.1)
A 		4.3
(-2.1)
A 		3.4
(-2.4)
A 		18
C 12.1
(3)
A 		6.2
(-0.6)
A 		6.9
(-0.2)
A 		2.4
(-2.9)
A 		3
(-2.6)
A 		2.3
(-3)
A 		10
avr 11.5
(3) 		5.3
(-1.2) 		3.7
(-2.3) 		2.9
(-2.8) 		3.1
(-2.7) 		2.6
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176287_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15778
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.1 NCBI
Target Description Y50D4B.1 /REP_DB=WormBase Gene ID /WP=CE26132 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070979(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070979 NCBI Caenorhabditis elegans Y50D4B.1 (Y50D4B.1) mRNA, complete cds. 11/11 None
Y50D4B.1 ENSEMBL cdna:known chromosome:CEL140:V:1111764:1113006:1 gene:Y50D4B.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000024555 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1111763-1113006(+) 99.83 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4B.1
Entrez Gene 190093 Entrez gene
SwissProt Q9N4S8 EMBL-EBI
WormBase CE26132 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59497 Hypothetical protein Y50D4B.1 [Caenorhabditis elegans] ref|NP_503380.1| Y50D4B.1 [Caenorhabditis elegans] 1.0E-109
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503380.1 1 4-26
Y50D4B.1 1 4-26
Sequence
Target Sequence 
>C. ELEGANS:176287_AT
aaccaaaatgtcagcccagttcttccgtctattcacttctaaaccaaaaaaaaatgctct
aaattccaatttcctctaccacttcctccaacataatgacacattactaatcttttcttt
gcataggcacacccttcgaaattttcttccacttggcaagattgtacacaaacaaaaaat
cttgctcttttgccccccacgcgattgtacaagttgctccgcaaagaaatctttaaaaaa
atggctgagtggggctaccaaaatcgcgagaaacagattcatgagaaggtgtttgacacg
ctttcccgtccattatcccgtgccagcaaagcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCAAAATGTCAGCCCAGTTCTTC 568 147 198 Antisense
TTCCGTCTATTCACTTCTAAACCAA 93 699 220 Antisense
TCTAAATTCCAATTTCCTCTACCAC 692 609 255 Antisense
CACATTACTAATCTTTTCTTTGCAT 51 197 297 Antisense
CTTTGCATAGGCACACCCTTCGAAA 703 219 314 Antisense
TCTTCCACTTGGCAAGATTGTACAC 108 619 342 Antisense
CCCCCACGCGATTGTACAAGTTGCT 159 261 391 Antisense
GCGATTGTACAAGTTGCTCCGCAAA 697 287 398 Antisense
GTGGGGCTACCAAAATCGCGAGAAA 567 487 446 Antisense
AGAAGGTGTTTGACACGCTTTCCCG 412 75 481 Antisense
CCATTATCCCGTGCCAGCAAAGCGA 72 271 507 Antisense
Sequence Source Affymetrix Proprietary Database