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[176285_s_at]

Description Y71H2AM.K /REP_DB=WormBase Gene ID /WP=CE22953 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 12      4grp_high_8_8 > 31      6grp_highest_nonorm > 79      6grp_total > 85      4grp_total > 32      6grp_highest_sub > In      6grp_high1_sub > In      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 713
1 2 3 4 range
A 911.5
(-3)
P 		1182.2
(-0.7)
P 		1612
(3)
P 		1247.6
(-0.1)
P 		701
B 899
(-3)
P 		1131.5
(1)
P 		1196
(2.2)
P 		1244.2
(3)
P 		345
C 929.2
(-3)
P 		1053.5
(-0.3)
P 		1001.4
(-1.5)
P 		1210
(3)
P 		281
avr 913.2
(-3) 		1122.4
(0.5) 		1269.8
(3) 		1233.9
(2.4) 		357

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1848
1 2 3 4 5 6 range
A 843.4
(-3)
P 		1128.4
(-2)
P 		1089.9
(-2.2)
P 		2001.8
(0.9)
P 		2152.7
(1.4)
P 		2638.8
(3)
P 		1795
B 801.1
(-2.9)
P 		791
(-3)
P 		1738.9
(1.7)
P 		1993.4
(3)
P 		1950.4
(2.8)
P 		1748.3
(1.8)
P 		1202
C 1283.9
(-0.6)
P 		846.5
(-3)
P 		1881.2
(2.6)
P 		1316.7
(-0.4)
P 		1949.6
(3)
P 		1909.4
(2.8)
P 		1103
avr 976.1
(-2.7) 		922
(-3) 		1570
(0.3) 		1770.6
(1.3) 		2017.6
(2.6) 		2098.8
(3) 		1177
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 239
1 2 3 4 range
A 331.9
(0.8)
P 		256.6
(-1.1)
P 		421.4
(3)
P 		182.6
(-3)
P 		239
B 286.7
(3)
P 		279.5
(1)
P 		277.2
(0.4)
P 		265.1
(-3)
P 		22
C 307.6
(1.3)
P 		302.9
(1.1)
P 		349
(3)
P 		204
(-3)
P 		145
avr 308.7
(1.2) 		279.7
(-0.2) 		349.2
(3) 		217.2
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 637
1 2 3 4 5 6 range
A 155.5
(-3)
P 		401.3
(-0)
P 		237.4
(-2)
P 		507.7
(1.3)
P 		649.6
(3)
P 		642.5
(2.9)
P 		494
B 278.7
(-2.8)
P 		263.3
(-3)
P 		422.3
(-1.2)
P 		528
(0)
P 		792.4
(3)
P 		651.9
(1.4)
P 		529
C 475.9
(0.4)
P 		258.3
(-3)
P 		478.8
(0.5)
P 		264.8
(-2.9)
P 		630.9
(2.9)
P 		639.2
(3)
P 		381
avr 303.4
(-3) 		307.6
(-2.9) 		379.5
(-1.8) 		433.5
(-1) 		691
(3) 		644.5
(2.3) 		388
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176285_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18946
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.K NCBI
Target Description Y71H2AM.K /REP_DB=WormBase Gene ID /WP=CE22953 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065208(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065208 NCBI Caenorhabditis elegans Y71H2AM.13 (Y71H2AM.13) mRNA, complete cds. 11/11 None
SNAP00000014441 ENSEMBL cdna:SNAP chromosome:CEL140:III:2734140:2739520:-1 11/11 None
GENEFINDER00000014470 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2734140:2739520:-1 11/11 None
Y71H2AM.13.1 ENSEMBL cdna:known chromosome:CEL140:III:2734068:2739521:-1 gene:Y71H2AM.13 11/11 None
Y71H2AM.13.2 ENSEMBL cdna:known chromosome:CEL140:III:2734069:2739523:-1 gene:Y71H2AM.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2734139-2739520(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.13
Entrez Gene 175390 Entrez gene
SwissProt Q9BL43 EMBL-EBI
WormBase CE22953 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1639001_A_AT Esterase-9 dm
DROSGENOME1:143823_AT Esterase-9 dm
Protein Similarities
Method ID Description E-Value
blast AAK29968 Hypothetical protein Y71H2AM.13 [Caenorhabditis elegans] ref|NP_497609.1| Y71H2AM.13 [Caenorhabditis elegans] 0.0
blast CAE65004 Hypothetical protein CBG09842 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.5E-16
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176285_S_AT
tcaacaagacgtctctgcaattctctctgaaaagctgttccccctgaaagctcgagacta
tcaagaaaaagctctggaaaaactgatcgaaatcggaacagatcattcgaaagaagaatg
gcaacgagcgatggttgatttgaagggcgactcatttttgaatgtcggaatacaacagaa
tgttctggatgtattggagactcaaccatcaactcctatttatatgtattcgtttgatta
ttgcaatccaaaagcttatggaattatgggatttaagttgccgtttaaagacgctaccca
ctgtactgatatcagttatgttgtcggaaatcatattgtcaattctttcgatttcaatga
ggaagactataaaatgattgagatcaccactcgattatggaccaatttcgcgaaatatgg
aaacccaaatggcgagggtgatgacgtggcacatctggaagagaaatgggagccggccac
aatcgaatccccacagacacacatggcactaacattacaaccaaagcttcatcacgtcta
caagcaaggaagaccactgttcatggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAACAAGACGTCTCTGCAATTCTC 64 631 1059 Antisense
AATTCTCTCTGAAAAGCTGTTCCCC 340 177 1077 Antisense
TCCCCCTGAAAGCTCGAGACTATCA 464 595 1097 Antisense
TTGCCGTTTAAAGACGCTACCCACT 595 229 1336 Antisense
ACGCTACCCACTGTACTGATATCAG 610 91 1349 Antisense
GAGATCACCACTCGATTATGGACCA 325 397 1438 Antisense
TGGCGAGGGTGATGACGTGGCACAT 483 545 1488 Antisense
AAATGGGAGCCGGCCACAATCGAAT 353 121 1522 Antisense
TCCCCACAGACACACATGGCACTAA 524 595 1546 Antisense
ACAACCAAAGCTTCATCACGTCTAC 567 113 1575 Antisense
GCAAGGAAGACCACTGTTCATGGCA 1 321 1602 Antisense
Sequence Source Affymetrix Proprietary Database