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[176284_s_at]

Description Y69A2AR.C /REP_DB=WormBase Gene ID /WP=CE26559 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 87      4grp_high_8_8 > 52      6grp_highest_nonorm > 92      6grp_total > 87      4grp_total > 59      6grp_highest_sub > Da      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 208
1 2 3 4 range
A 189.4
(2.1)
P 		212.6
(3)
P 		181.4
(1.8)
P 		61.2
(-3)
A 		151
B 145.6
(-1.3)
P 		212.4
(1)
P 		269.1
(3)
P 		97.8
(-3)
P 		171
C 220.4
(1.7)
P 		239.4
(3)
P 		169.6
(-2)
P 		154.8
(-3)
A 		85
avr 185.1
(1.1) 		221.5
(3) 		206.7
(2.2) 		104.6
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 314
1 2 3 4 5 6 range
A 182.9
(-2.4)
P 		154.1
(-3)
P 		165.4
(-2.8)
P 		232.4
(-1.4)
P 		267.6
(-0.7)
P 		448.5
(3)
P 		294
B 200.7
(-1.5)
P 		134.8
(-3)
P 		179
(-2)
P 		201.8
(-1.5)
P 		381.1
(2.5)
P 		401.4
(3)
P 		267
C 291.3
(-0.2)
P 		168
(-3)
P 		185.3
(-2.6)
P 		305.9
(0.1)
P 		260.1
(-0.9)
P 		437
(3)
P 		269
avr 225
(-1.4) 		152.3
(-3) 		176.6
(-2.5) 		246.7
(-1) 		302.9
(0.3) 		429
(3) 		277
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 68.9
(3)
P 		46.1
(0.7)
P 		47.4
(0.8)
P 		9
(-3)
A 		60
B 46.4
(0.7)
P 		52.5
(1.6)
P 		62.4
(3)
P 		20.8
(-3)
P 		42
C 73
(3)
P 		68.8
(2.5)
P 		59.1
(1.2)
P 		26.1
(-3)
A 		47
avr 62.8
(3) 		55.8
(2) 		56.3
(2.1) 		18.6
(-3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 33.7
(-3)
P 		54.8
(-1.3)
P 		36
(-2.8)
P 		58.9
(-1)
P 		80.8
(0.7)
P 		109.2
(3)
P 		76
B 69.8
(-1.6)
P 		44.9
(-2.9)
P 		43.5
(-3)
P 		53.4
(-2.5)
P 		154.9
(3)
P 		149.7
(2.7)
P 		111
C 108
(0.7)
P 		51.3
(-2.8)
P 		47.2
(-3)
P 		61.5
(-2.1)
P 		84.2
(-0.8)
P 		146.3
(3)
P 		99
avr 70.5
(-1.2) 		50.3
(-2.5) 		42.2
(-3) 		57.9
(-2) 		106.6
(1.2) 		135.1
(3) 		93
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176284_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19333
Sequence Type Exemplar sequence
Representative Public ID Y69A2AR.C NCBI
Target Description Y69A2AR.C /REP_DB=WormBase Gene ID /WP=CE26559 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028392(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028392 NCBI Caenorhabditis elegans MAD (yeast Mitosis Arrest DeFicient) related family member (mdf-2) (mdf-2) mRNA, complete cds. 11/11 None
SNAP00000018824 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2635222:2655555:-1 10/11 None
Y69A2AR.30a.2 ENSEMBL cdna:known chromosome:CEL140:IV:2636090:2641020:-1 gene:Y69A2AR.30 11/11 None
Y69A2AR.30a.1 ENSEMBL cdna:known chromosome:CEL140:IV:2636243:2641021:-1 gene:Y69A2AR.30 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171291 6/11 Cross Hyb Matching Probes None
GENEFINDER00000018833 5/11 Cross Hyb Matching Probes None
GENEFINDER00000018856 5/11 Cross Hyb Matching Probes None
Y69A2AR.30b 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177046 2 1 NM_001028392 176284_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2636552-2641013(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y69A2AR.30
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68577 Hypothetical protein Y69A2AR.31 [Caenorhabditis elegans] ref|NP_500240.1| Y69A2AR.31 [Caenorhabditis elegans] 0.0
blast CAE68242 Hypothetical protein CBG13916 [Caenorhabditis briggsae] 0.0
blast AAK68576 Mad (yeast mitosis arrest deficient) related protein 2, isoform a [Caenorhabditis elegans] ref|NP_001023563.1| MAD (yeast Mitosis Arrest DeFicient) related family member (mdf-2) [Caenorhabditis elegans] gb|AAF63495.1| MDF-2 [Caenorhabditis elegans] 1.0E-111
blast CAE68212 Hypothetical protein CBG13880 [Caenorhabditis briggsae] 1.0E-102
blast AAK68576 Mad (yeast mitosis arrest deficient) related protein 2, isoform a [Caenorhabditis elegans] ref|NP_001023563.1| MAD (yeast Mitosis Arrest DeFicient) related family member (mdf-2) [Caenorhabditis elegans] gb|AAF63495.1| MDF-2 [Caenorhabditis elegans] 1.0E-97
blast AAM15619 Mad (yeast mitosis arrest deficient) related protein 2, isoform b [Caenorhabditis elegans] ref|NP_741342.2| MAD (yeast Mitosis Arrest DeFicient) related family member (mdf-2) [Caenorhabditis elegans] 3.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR003511 EMBL-EBI DNA-binding HORMA 1.3E-78
Pfam IPR003511 EMBL-EBI DNA-binding HORMA 3.9E-67
Sequence
Target Sequence 
>C. ELEGANS:176284_S_AT
gcgatttccctcaaaggatctgcccaactcgtcaaagagttcttccatttcggccttaac
agcattctctatcaacgtgcgctttatccatcagacagtttcaagcgtgagaagaagtac
gggctcacactgtgggtggctcatgagaagaagctccaagcgttcatggatccacttttg
cagcaagttgaatattggctcgcgaaaagacagctgaaacggttggtgatggtgatttcc
gaggtgaagacgaaggaagtcgtggaaagatggcaattcgatattcatacagagaatttg
gccgaggaaggagaaaacgcncaccgcgtcaaggaggagaagaagattcggcaggagatt
agcgatgtgattcgccaaatcaccgcatctgtgtcattcttgccactgctcgaggagcct
gtctcgttcgatgtgctcatttacactggaaaagatactcaagcacccgaggactggact
gaaagcggagcctgtttgattcagaattcggagactgtgcaacttcgctcattctcgacg
tcagttcactcggttaa

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGATTTCCCTCAAAGGATCTGCCC 466 289 37 Antisense
ATTTCGGCCTTAACAGCATTCTCTA 354 17 83 Antisense
CATTCTCTATCAACGTGCGCTTTAT 222 217 99 Antisense
TGCGCTTTATCCATCAGACAGTTTC 49 585 114 Antisense
GCTCACACTGTGGGTGGCTCATGAG 578 301 159 Antisense
GCGTTCATGGATCCACTTTTGCAGC 129 291 196 Antisense
AAATCACCGCATCTGTGTCATTCTT 298 117 413 Antisense
CTCGAGGAGCCTGTCTCGTTCGATG 437 233 445 Antisense
GATACTCAAGCACCCGAGGACTGGA 35 425 490 Antisense
GGACTGGACTGAAAGCGGAGCCTGT 312 525 507 Antisense
TCTCGACGTCAGTTCACTCGGTTAA 5 611 569 Antisense
Sequence Source Affymetrix Proprietary Database