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[176277_at]

Description Y71H2AM.B /REP_DB=WormBase Gene ID /WP=CE22937 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 62      4grp_total > 35      6grp_cgc > 83      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 12.7
(-3)
A 		28.8
(3)
A 		14.6
(-2.3)
A 		14.7
(-2.3)
A 		16
B 18.6
(3)
A 		13.2
(-0.8)
A 		10
(-3)
A 		17.2
(2)
A 		9
C 10.8
(-3)
A 		24.8
(3)
A 		18.4
(0.3)
A 		17.8
(0)
A 		14
avr 14
(-3) 		22.3
(3) 		14.3
(-2.8) 		16.6
(-1.1) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 23.3
(2.6)
A 		13.3
(-2.3)
A 		21.8
(1.8)
A 		11.8
(-3)
A 		24.2
(3)
A 		17.2
(-0.4)
A 		12
B 13.5
(-1.2)
A 		14.8
(-0.2)
A 		19.4
(3)
A 		17.6
(1.7)
A 		15.7
(0.4)
A 		10.9
(-3)
A 		9
C 9.7
(-2.7)
A 		23.3
(1.2)
A 		18.7
(-0.1)
A 		29.6
(3)
A 		8.5
(-3)
A 		16.7
(-0.7)
A 		21
avr 15.5
(-2.3) 		17.1
(-0.4) 		20
(3) 		19.7
(2.6) 		16.1
(-1.6) 		14.9
(-3) 		5
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 4.6
(0.7)
A 		6.2
(3)
A 		3.8
(-0.5)
A 		2.1
(-3)
A 		4
B 5.9
(3)
A 		3.3
(-1.3)
A 		2.3
(-3)
A 		3.7
(-0.7)
A 		4
C 3.6
(-2.1)
A 		7.1
(3)
A 		6.4
(2)
A 		3
(-3)
A 		4
avr 4.7
(1.2) 		5.5
(3) 		4.2
(0) 		2.9
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 4.3
(-1.2)
A 		4.7
(-0.6)
A 		4.7
(-0.6)
A 		3
(-3)
A 		7.3
(3)
A 		4.2
(-1.3)
A 		4
B 4.7
(-1.4)
A 		4.9
(-0.9)
A 		4.7
(-1.4)
A 		4.7
(-1.4)
A 		6.4
(3)
A 		4.1
(-3)
A 		2
C 3.6
(-1.8)
A 		7.1
(3)
A 		4.8
(-0.1)
A 		5.9
(1.4)
A 		2.7
(-3)
A 		5.6
(1)
A 		4
avr 4.2
(-3) 		5.6
(3) 		4.7
(-0.9) 		4.5
(-1.7) 		5.5
(2.6) 		4.6
(-1.3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176277_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18961
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.B NCBI
Target Description Y71H2AM.B /REP_DB=WormBase Gene ID /WP=CE22937 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065220(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065220 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-45) (twk-45) mRNA, complete cds. 11/11 None
GENEFINDER00000014485 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2793333:2800858:-1 11/11 None
SNAP00000014453 ENSEMBL cdna:SNAP chromosome:CEL140:III:2798031:2801537:-1 11/11 None
Y71H2AM.22 ENSEMBL cdna:known chromosome:CEL140:III:2798963:2800858:-1 gene:Y71H2AM.22 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2798962-2800858(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-45
Ensembl Y71H2AM.22
Entrez Gene 190614 Entrez gene
SwissProt Q9BL31 EMBL-EBI
WormBase CE39072 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE68368 Hypothetical protein CBG14123 [Caenorhabditis briggsae] 1.0E-164
blast AAC32864 putative potassium channel subunit n2P26 [Caenorhabditis elegans] 1.0E-163
blast AAK39200 Twik family of potassium channels protein 26 [Caenorhabditis elegans] ref|NP_508522.2| TWiK family of potassium channels family member (twk-26) [Caenorhabditis elegans] pir||T16629 hypothetical protein M02F4.5 - Caenorhabditis elegans 1.0E-163
blast NP_497621 TWiK family of potassium channels family member (twk-45) [Caenorhabditis elegans] 1.0E-156
blast AAK29962 Twik family of potassium channels protein 45 [Caenorhabditis elegans] 1.0E-143
blast NP_497621 TWiK family of potassium channels family member (twk-45) [Caenorhabditis elegans] 1.0E-142
blast AAK29962 Twik family of potassium channels protein 45 [Caenorhabditis elegans] 1.0E-129
blast NP_497621 TWiK family of potassium channels family member (twk-45) [Caenorhabditis elegans] 1.0E-126
blast AAK29962 Twik family of potassium channels protein 45 [Caenorhabditis elegans] 1.0E-112
blast AAK39200 Twik family of potassium channels protein 26 [Caenorhabditis elegans] ref|NP_508522.2| TWiK family of potassium channels family member (twk-26) [Caenorhabditis elegans] pir||T16629 hypothetical protein M02F4.5 - Caenorhabditis elegans 1.0E-105
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497621.2 4 47-69,166-188,198-220,268-290
Y71H2AM.22 3 47-69,166-188,198-220
GENEFINDER00000014485 6 47-69,166-188,198-220,270-292,307-326,333-355
SNAP00000014453 4 62-84,181-203,213-235,266-288
Sequence
Target Sequence 
>C. ELEGANS:176277_AT
aaaaaatcacttggttcttcgcctggcttgcatattatcaccacaagtttggaattagac
atatcacacttatatcaatactcgctgcctatatttgcctcggaggatttttgtttcaga
aattggaatcaccgcgggaaattgaggtagatttctgtatcctgagtaatcgttttaatt
ctttgtgcgatcaggaattacaagagacattaaagtcgatgaatgaaataattaaaaacg
agactatggatataataaaaatcacactaacgactaatggagaggacagaaatcaaaaat
tgggggatttactgagagcctaccaccgaatcctcctggaaaccgaaggaaaattccacg
gcagcgcctggcacaaatcggaaaacctcgacatgaacctaatgtggtatttctcatctg
ccactttctactcaatgaccttattctccacaattggttacggtactatcacctgtcaaa
ccttttggggaaaaactgtctcaatggtgtatgcatcaattggtcttcctatcatgctcc
ttgtgctcggagaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAAATCACTTGGTTCTTCGCCTG 30 131 104 Antisense
GTTCTTCGCCTGGCTTGCATATTAT 522 443 117 Antisense
TCAATACTCGCTGCCTATATTTGCC 636 633 178 Antisense
GGGGGATTTACTGAGAGCCTACCAC 217 491 405 Antisense
GAAGGAAAATTCCACGGCAGCGCCT 76 333 448 Antisense
AGCGCCTGGCACAAATCGGAAAACC 369 81 466 Antisense
TCATCTGCCACTTTCTACTCAATGA 362 621 517 Antisense
ATGACCTTATTCTCCACAATTGGTT 100 45 538 Antisense
GTTACGGTACTATCACCTGTCAAAC 247 437 560 Antisense
TCAATTGGTCTTCCTATCATGCTCC 29 635 619 Antisense
ATCATGCTCCTTGTGCTCGGAGACA 27 31 634 Antisense
Sequence Source Affymetrix Proprietary Database