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[176275_at]

Description Y71H2AM.V /REP_DB=WormBase Gene ID /WP=CE26273 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 45      4grp_total > 22      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 57.6
(-1.1)
A 		41.2
(-2.4)
A 		33.9
(-3)
A 		109.3
(3)
A 		75
B 131
(3)
A 		41.9
(-1.9)
A 		42.8
(-1.8)
A 		21.3
(-3)
A 		110
C 53.6
(-0.6)
A 		17.5
(-3)
A 		18.5
(-2.9)
A 		108
(3)
A 		91
avr 80.7
(3) 		33.5
(-2.8) 		31.7
(-3) 		79.5
(2.9) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 12.4
(-1.7)
A 		34.1
(1.4)
A 		45.5
(3)
A 		8.5
(-2.2)
A 		3
(-3)
A 		27.6
(0.5)
A 		43
B 60.3
(3)
A 		35.5
(-0.1)
A 		47
(1.3)
A 		12.3
(-3)
A 		34.7
(-0.2)
A 		23.5
(-1.6)
A 		48
C 26.5
(-0.3)
A 		39.6
(3)
A 		16.2
(-2.9)
A 		29.5
(0.5)
A 		15.6
(-3)
A 		35.9
(2.1)
A 		24
avr 33.1
(2) 		36.4
(3) 		36.2
(2.9) 		16.8
(-3) 		17.8
(-2.7) 		29
(0.7) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 21
(3)
A 		8.9
(-3)
A 		8.9
(-3)
A 		16
(0.5)
A 		12
B 41.8
(3)
A 		10.3
(-2.1)
A 		9.9
(-2.1)
A 		4.5
(-3)
A 		37
C 17.7
(2.8)
A 		5
(-3)
A 		6.5
(-2.3)
A 		18.2
(3)
A 		13
avr 26.8
(3) 		8.1
(-3) 		8.4
(-2.9) 		12.9
(-1.5) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 2.3
(-2.3)
A 		12.1
(3)
A 		9.9
(1.8)
A 		2.2
(-2.3)
A 		0.9
(-3)
A 		6.7
(0.1)
A 		11
B 21
(3)
A 		11.8
(-0.1)
A 		11.4
(-0.3)
A 		3.3
(-3)
A 		14.1
(0.7)
A 		8.8
(-1.1)
A 		18
C 9.8
(1.3)
A 		12.1
(3)
A 		4.1
(-3)
A 		5.9
(-1.7)
A 		5.1
(-2.3)
A 		12
(2.9)
A 		8
avr 11
(2.3) 		12
(3) 		8.5
(0.4) 		3.8
(-3) 		6.7
(-0.9) 		9.2
(1) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176275_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18934
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.V NCBI
Target Description Y71H2AM.V /REP_DB=WormBase Gene ID /WP=CE26273 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065223(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065223 NCBI Caenorhabditis elegans Y71H2AM.2 (Y71H2AM.2) mRNA, complete cds. 11/11 None
SNAP00000014457 ENSEMBL cdna:SNAP chromosome:CEL140:III:2819272:2832868:1 9/11 None
GENEFINDER00000014460 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2819272:2833773:1 10/11 None
Y71H2AM.2 ENSEMBL cdna:known chromosome:CEL140:III:2819272:2824171:1 gene:Y71H2AM.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2819271-2824171(+) 82.6 98.06
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.2
Entrez Gene 190605 Entrez gene
SwissProt Q9BL29 EMBL-EBI
WormBase CE26273 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29978 Hypothetical protein Y71H2AM.1 [Caenorhabditis elegans] ref|NP_497625.3| Y71H2AM.1 [Caenorhabditis elegans] 1.0E-178
blast AAK29978 Hypothetical protein Y71H2AM.1 [Caenorhabditis elegans] ref|NP_497625.3| Y71H2AM.1 [Caenorhabditis elegans] 1.0E-176
blast AAK29977 Hypothetical protein Y71H2AM.2 [Caenorhabditis elegans] ref|NP_497624.1| Y71H2AM.2 [Caenorhabditis elegans] 1.0E-167
blast CAE57513 Hypothetical protein CBG00488 [Caenorhabditis briggsae] 1.0E-156
blast AAH54300 Osgep-prov protein [Xenopus laevis] sp|Q7SYR1|GCP_XENLA Probable O-sialoglycoprotein endopeptidase 1.0E-123
blast NP_001016112 O-sialoglycoprotein endopeptidase [Xenopus tropicalis] 1.0E-123
blast AAH54300 Osgep-prov protein [Xenopus laevis] sp|Q7SYR1|GCP_XENLA Probable O-sialoglycoprotein endopeptidase 1.0E-121
blast CAE57515 Hypothetical protein CBG00490 [Caenorhabditis briggsae] 1.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR000905 EMBL-EBI Peptidase M22, glycoprotease 1.9E-90
Pfam IPR000905 EMBL-EBI Peptidase M22, glycoprotease 7.3E-92
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497624.1 2 91-113,277-299
Y71H2AM.2 2 91-113,277-299
SNAP00000014457 2 187-206,216-234
GENEFINDER00000014460 6 91-113,213-235,285-307,385-407,458-476,873-895
Sequence
Target Sequence 
>C. ELEGANS:176275_AT
gtccagctccacccggaaagaaatatgtgagatgtccgtgtaattgcctattgatctgca
aggcttcatctacgagaattgcctgcccacgcaataattgccgtcgggttatcactttgg
attttttgatagaaaaatcgaatatttttgcgattttaatgaaaaaagccggtcaacggg
acccgggaacagcaattcgtgcaccaacaggaagttgccgtgtcaaatgtaatcattgca
atgagatattcctattcaatacactgacaaatgcgctggcaaattgtccacattgcaaga
aatattcgactgtcggaagttttgctcgtcgtcgtgctcttttattctttattcttgctg
caactgttgctgtgttggctgttattttgacaatgttgacgaatgcatcggtagggccag
ttttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCAGCTCCACCCGGAAAGAAATA 464 477 503 Antisense
GTGAGATGTCCGTGTAATTGCCTAT 543 483 529 Antisense
ATTGATCTGCAAGGCTTCATCTACG 159 5 552 Antisense
TTCATCTACGAGAATTGCCTGCCCA 146 685 567 Antisense
GCCTGCCCACGCAATAATTGCCGTC 455 281 583 Antisense
TGCCGTCGGGTTATCACTTTGGATT 383 581 601 Antisense
GACCCGGGAACAGCAATTCGTGCAC 591 379 682 Antisense
AAATGCGCTGGCAAATTGTCCACAT 422 119 771 Antisense
GACTGTCGGAAGTTTTGCTCGTCGT 410 375 810 Antisense
GCTGCAACTGTTGCTGTGTTGGCTG 440 297 859 Antisense
GAATGCATCGGTAGGGCCAGTTTTC 580 325 903 Antisense
Sequence Source Affymetrix Proprietary Database