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[176274_at]

Description Y52E8A.4 /REP_DB=WormBase Gene ID /WP=CE26156 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 28.9
(-3)
P 		74.4
(0.3)
P 		69.1
(-0.1)
M 		111.1
(3)
P 		82
B 67
(3)
P 		61.4
(0.8)
P 		51.6
(-3)
A 		53.9
(-2.1)
P 		15
C 31.8
(-2.1)
P 		33.6
(-2)
A 		14.5
(-3)
A 		131.9
(3)
P 		117
avr 42.6
(-3) 		56.5
(-1.5) 		45.1
(-2.7) 		99
(3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 53.2
(3)
A 		28.2
(-1.8)
P 		45.7
(1.5)
M 		53.2
(3)
A 		43.1
(1)
A 		22.2
(-3)
A 		31
B 51.5
(3)
A 		49.9
(2.7)
P 		19.6
(-3)
A 		35.9
(0.1)
A 		50.5
(2.8)
P 		42
(1.2)
P 		32
C 18.1
(-3)
A 		56.8
(3)
P 		27.6
(-1.5)
A 		38.2
(0.1)
A 		39.7
(0.3)
P 		51.7
(2.2)
A 		39
avr 40.9
(1.2) 		45
(3) 		31
(-3) 		42.4
(1.9) 		44.4
(2.7) 		38.6
(0.3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 10.5
(-3)
P 		16.1
(1.4)
P 		18.1
(3)
M 		16.3
(1.6)
P 		8
B 21.4
(3)
P 		15.2
(-0.8)
P 		12
(-2.7)
A 		11.5
(-3)
P 		10
C 10.5
(-1.1)
P 		9.7
(-1.4)
A 		5.1
(-3)
A 		22.2
(3)
P 		17
avr 14.1
(-0.1) 		13.7
(-0.6) 		11.7
(-3) 		16.7
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 9.8
(0.3)
A 		10
(0.4)
P 		10
(0.4)
M 		13.5
(3)
A 		13
(2.6)
A 		5.4
(-3)
A 		8
B 17.9
(2)
A 		16.6
(1.5)
P 		4.8
(-3)
A 		9.5
(-1.2)
A 		20.5
(3)
P 		15.7
(1.2)
P 		16
C 6.7
(-3)
A 		17.3
(3)
P 		7
(-2.8)
A 		7.7
(-2.4)
A 		12.8
(0.5)
P 		17.3
(3)
A 		11
avr 11.5
(0.1) 		14.6
(2.4) 		7.3
(-3) 		10.2
(-0.9) 		15.4
(3) 		12.8
(1.1) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176274_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5202
Sequence Type Exemplar sequence
Representative Public ID Y52E8A.4 NCBI
Target Description Y52E8A.4 /REP_DB=WormBase Gene ID /WP=CE26156 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062357(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062357 NCBI Caenorhabditis elegans Y52E8A.4 (Y52E8A.4) mRNA, complete cds. 11/11 None
SNAP00000040245 ENSEMBL cdna:SNAP chromosome:CEL140:II:4309717:4312591:-1 11/11 None
GENEFINDER00000040252 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4309717:4312207:-1 11/11 None
Y52E8A.4 ENSEMBL cdna:known chromosome:CEL140:II:4309829:4312207:-1 gene:Y52E8A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4309827-4312206(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y52E8A.4
Entrez Gene 190188 Entrez gene
SwissProt Q965T3 EMBL-EBI
WormBase CE26156 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84613 Hypothetical protein Y52E8A.4 [Caenorhabditis elegans] ref|NP_494758.1| Y52E8A.4 [Caenorhabditis elegans] 0.0
blast AAK84613 Hypothetical protein Y52E8A.4 [Caenorhabditis elegans] ref|NP_494758.1| Y52E8A.4 [Caenorhabditis elegans] 1.0E-170
blast AAC71106 Hypothetical protein F31D5.1 [Caenorhabditis elegans] ref|NP_494742.1| F31D5.1 [Caenorhabditis elegans] 1.0E-146
blast AAC71106 Hypothetical protein F31D5.1 [Caenorhabditis elegans] ref|NP_494742.1| F31D5.1 [Caenorhabditis elegans] 1.0E-125
blast CAE74272 Hypothetical protein CBG21963 [Caenorhabditis briggsae] 1.0E-121
blast AAC71106 Hypothetical protein F31D5.1 [Caenorhabditis elegans] ref|NP_494742.1| F31D5.1 [Caenorhabditis elegans] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 5.6E-93
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 7.9E-28
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 1.3E-27
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 7.9E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494758.1 12 13-32,52-74,81-98,103-122,144-163,178-197,228-250,265-287,299-318,333-355,375-397,402-419
Y52E8A.4 12 13-32,52-74,81-98,103-122,144-163,178-197,228-250,265-287,299-318,333-355,375-397,402-419
SNAP00000040245 8 15-34,75-94,109-128,159-181,196-218,230-249,264-286,317-339
GENEFINDER00000040252 10 13-32,52-69,133-152,167-186,217-239,254-276,288-307,322-344,364-386,391-408
Sequence
Target Sequence 
>C. ELEGANS:176274_AT
ggatgttctgctttacgattttctttttctacaccggattgatcaggtctttctggattt
caatctacccggcatgcatcaaattcacatctcaactttccaccaatacaactgctatcc
taactatcggaatgattgtcactggctgcggacaggttatcggatcactttcagtcgcag
tcattggaaataaaatccgaaaattcggccagcatactctcattctctgtgctctcattt
tacacattttcttgtgtgttatgatctccttgacattccccaatgatgctccactgggac
atactgataagaatggtccagtttttggcgcgagtgtgctctctgcgatgactatttctg
cattattgggcttcggagatgctattctgcagacacaagtttattcttatatcgccaaat
attatcagaatgagagcagcactgttttttcgattttcagattttctagtggaattgctt
ctactctacttttcttcgctgcacaatatttccatttgatgcaccatctgattttaatag
cgattttcgcagttctcagtgggctggccgttttgaggtttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGTTCTGCTTTACGATTTTCTT 177 515 689 Antisense
TTTCAATCTACCCGGCATGCATCAA 219 671 746 Antisense
GGTTATCGGATCACTTTCAGTCGCA 446 505 843 Antisense
GAAAATTCGGCCAGCATACTCTCAT 469 353 887 Antisense
CATTCTCTGTGCTCTCATTTTACAC 29 217 909 Antisense
GTGTTATGATCTCCTTGACATTCCC 635 485 944 Antisense
TGCTCTCTGCGATGACTATTTCTGC 230 581 1025 Antisense
TGGAATTGCTTCTACTCTACTTTTC 166 547 1158 Antisense
TCTACTTTTCTTCGCTGCACAATAT 529 613 1173 Antisense
CGATTTTCGCAGTTCTCAGTGGGCT 113 249 1229 Antisense
TGGGCTGGCCGTTTTGAGGTTTCAA 485 551 1248 Antisense
Sequence Source Affymetrix Proprietary Database