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[176268_at]

Description Y82E9BL.C /REP_DB=WormBase Gene ID /WP=CE23419 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 84      4grp_total > 11      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 8.4
(-2.5)
A 		4
(-3)
A 		3.8
(-3)
A 		64.9
(3)
A 		61
B 20
(-3)
A 		36.9
(1.4)
A 		34
(0.6)
A 		43.3
(3)
A 		23
C 57.6
(-0.8)
A 		45.1
(-1.9)
A 		32.4
(-3)
A 		102.7
(3)
A 		70
avr 28.7
(-2.3) 		28.7
(-2.3) 		23.4
(-3) 		70.3
(3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 324
1 2 3 4 5 6 range
A 8.5
(-3)
A 		37.8
(-2.4)
A 		135.7
(-0.3)
P 		261.1
(2.5)
P 		286.1
(3)
P 		233
(1.9)
P 		278
B 20.7
(-3)
A 		53.3
(-2.4)
A 		94.5
(-1.6)
P 		233.8
(1.1)
P 		332.3
(3)
P 		227.7
(1)
P 		312
C 20.7
(-3)
A 		54.5
(-2.2)
A 		149
(-0.1)
P 		288.4
(3)
P 		265.8
(2.5)
P 		281.9
(2.9)
P 		268
avr 16.6
(-3) 		48.5
(-2.3) 		126.4
(-0.6) 		261.1
(2.3) 		294.7
(3) 		247.5
(2) 		278
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 3.1
(-1.5)
A 		0.9
(-3)
A 		1
(-2.9)
A 		9.5
(3)
A 		9
B 6.4
(-3)
A 		9.1
(2.8)
A 		7.9
(0.2)
A 		9.2
(3)
A 		3
C 19.1
(3)
A 		13
(-1.7)
A 		11.3
(-3)
A 		17.3
(1.6)
A 		8
avr 9.5
(0.2) 		7.7
(-1.9) 		6.7
(-3) 		12
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 1.6
(-3)
A 		13.4
(-2.2)
A 		29.6
(-1)
P 		66.2
(1.6)
P 		86.3
(3)
P 		56.7
(0.9)
P 		85
B 7.2
(-3)
A 		17.7
(-2.5)
A 		22.9
(-2.3)
P 		61.9
(-0.4)
P 		135
(3)
P 		84.9
(0.6)
P 		128
C 7.7
(-3)
A 		16.6
(-2.4)
A 		37.9
(-0.9)
P 		58
(0.5)
P 		86
(2.4)
P 		94.4
(3)
P 		87
avr 5.5
(-3) 		15.9
(-2.4) 		30.1
(-1.5) 		62
(0.5) 		102.4
(3) 		78.7
(1.5) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176268_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18982
Sequence Type Exemplar sequence
Representative Public ID Y82E9BL.C NCBI
Target Description Y82E9BL.C /REP_DB=WormBase Gene ID /WP=CE23419 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064990(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064990 NCBI Caenorhabditis elegans Y82E9BL.3 (Y82E9BL.3) mRNA, complete cds. 11/11 None
GENEFINDER00000041785 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1345341:1350826:-1 10/11 None
SNAP00000041774 ENSEMBL cdna:SNAP chromosome:CEL140:III:1349742:1350826:-1 9/11 None
Y82E9BL.3 ENSEMBL cdna:known chromosome:CEL140:III:1349742:1350826:-1 gene:Y82E9BL.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1349741-1350826(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y82E9BL.3
Entrez Gene 190752 Entrez gene
SwissProt Q9GR30 EMBL-EBI
WormBase CE23419 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG37970 Hypothetical protein Y82E9BL.3 [Caenorhabditis elegans] ref|NP_497391.1| Y82E9BL.3 [Caenorhabditis elegans] 1.0E-135
blast AAG37970 Hypothetical protein Y82E9BL.3 [Caenorhabditis elegans] ref|NP_497391.1| Y82E9BL.3 [Caenorhabditis elegans] 1.0E-134
blast AAK66032 Hypothetical protein Y82E9BL.5 [Caenorhabditis elegans] ref|NP_497389.1| Y82E9BL.5 [Caenorhabditis elegans] 1.0E-130
blast AAK66031 Hypothetical protein Y82E9BL.4 [Caenorhabditis elegans] ref|NP_497390.1| Y82E9BL.4 [Caenorhabditis elegans] 1.0E-102
blast AAG37970 Hypothetical protein Y82E9BL.3 [Caenorhabditis elegans] ref|NP_497391.1| Y82E9BL.3 [Caenorhabditis elegans] 5.0E-90
blast AAK66033 Hypothetical protein Y82E9BL.6 [Caenorhabditis elegans] ref|NP_497388.1| Y82E9BL.6 [Caenorhabditis elegans] 1.0E-85
blast AAK66033 Hypothetical protein Y82E9BL.6 [Caenorhabditis elegans] ref|NP_497388.1| Y82E9BL.6 [Caenorhabditis elegans] 6.0E-85
blast AAK66032 Hypothetical protein Y82E9BL.5 [Caenorhabditis elegans] ref|NP_497389.1| Y82E9BL.5 [Caenorhabditis elegans] 1.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.5E-7
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.4E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497391.1 6 7-29,39-58,78-100,105-127,149-171,181-203
Y82E9BL.3 6 7-29,39-58,78-100,105-127,149-171,181-203
GENEFINDER00000041785 11 15-32,52-74,84-103,124-146,156-178,452-474,484-501,514-536,546-568,590-612,627-649
SNAP00000041774 5 10-32,39-58,78-100,135-157,167-189
Sequence
Target Sequence 
>C. ELEGANS:176268_AT
actatccatgattgtgccttttctgtgggttgttataaataaagaatgcctaaaagtgca
acaatcgatcgaaaacttctactggcatcggatctttattgcaggtgcagtggtctgctt
gaatatattacttccgattgctatgattctcatgtttttcggaggatggataaatatgct
tgcttccatcctcatccattctctactaatattacagttaatatgtttcgcaaatgttct
tctagaatctgaatcacctatcaagtacactttcccaaaaaccacaaaactaaccaacct
tcaaaaggtttccatcttcatattcttcaccattatggaagtatactatatctacttgtg
cacctggactaattcttcttcaaacaattataacattattcatttcttgccaggaactat
atacctatgcccaataatcaatataatgtctataccagcagtcgtagttgcttgttatgc
acataactcggataaagttcatttatctcgtttgagacctgatggtggattagaacacat
tgagataagaacatgggaccctgcaactggaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTATCCATGATTGTGCCTTTTCTG 653 101 150 Antisense
AACTTCTACTGGCATCGGATCTTTA 422 139 223 Antisense
ACTTCCGATTGCTATGATTCTCATG 694 103 279 Antisense
ATAAATATGCTTGCTTCCATCCTCA 340 25 319 Antisense
TCCTCATCCATTCTCTACTAATATT 476 591 338 Antisense
TATGTTTCGCAAATGTTCTTCTAGA 95 655 371 Antisense
CAAAAGGTTTCCATCTTCATATTCT 35 191 451 Antisense
TGCACCTGGACTAATTCTTCTTCAA 31 579 508 Antisense
TAACATTATTCATTTCTTGCCAGGA 568 639 540 Antisense
GCAGTCGTAGTTGCTTGTTATGCAC 473 311 607 Antisense
AACATGGGACCCTGCAACTGGAATA 530 137 699 Antisense
Sequence Source Affymetrix Proprietary Database