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[176259_at]

Description Y50D4B.3 /REP_DB=WormBase Gene ID /WP=CE26134 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 32      6grp_total > 21      4grp_total > 60      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 45.6
(2.3)
P 		50.2
(3)
A 		29.5
(-0.1)
A 		10.6
(-3)
A 		40
B 77.1
(3)
P 		47.5
(0.3)
M 		61
(1.5)
P 		11.2
(-3)
A 		66
C 37.8
(-1.6)
A 		33.6
(-2)
A 		82.7
(3)
P 		23.5
(-3)
A 		59
avr 53.5
(2.4) 		43.8
(1) 		57.7
(3) 		15.1
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 211
1 2 3 4 5 6 range
A 102.3
(0)
P 		143.4
(1.9)
P 		167.8
(3)
P 		118.9
(0.8)
P 		58
(-2)
M 		36
(-3)
A 		132
B 96.8
(-0.9)
P 		196.9
(1.9)
P 		235.4
(3)
P 		102.2
(-0.8)
P 		99.6
(-0.9)
P 		24.7
(-3)
A 		211
C 129.3
(0.4)
P 		203.9
(3)
P 		169.3
(1.8)
P 		101.4
(-0.6)
P 		85.3
(-1.2)
P 		33.7
(-3)
P 		170
avr 109.5
(-0.1) 		181.4
(2.6) 		190.8
(3) 		107.5
(-0.1) 		81
(-1.1) 		31.5
(-3) 		159
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 16.6
(3)
P 		10.9
(0.7)
A 		7.7
(-0.6)
A 		1.6
(-3)
A 		15
B 24.6
(3)
P 		11.7
(-0.5)
M 		14.1
(0.2)
P 		2.4
(-3)
A 		22
C 12.5
(-0.9)
A 		9.7
(-1.6)
A 		28.8
(3)
P 		4
(-3)
A 		25
avr 17.9
(3) 		10.8
(0.2) 		16.9
(2.6) 		2.7
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 18.9
(-1.6)
P 		51
(3)
P 		36.6
(1)
P 		30.2
(0)
P 		17.5
(-1.8)
M 		8.8
(-3)
A 		42
B 33.7
(-0.4)
P 		65.6
(3)
P 		57.2
(2.1)
P 		27.1
(-1.1)
P 		40.5
(0.3)
P 		9.2
(-3)
A 		56
C 47.9
(1.3)
P 		62.2
(3)
P 		43.1
(0.7)
P 		20.4
(-1.9)
P 		27.6
(-1.1)
P 		11.3
(-3)
P 		51
avr 33.5
(-0.1) 		59.6
(3) 		45.6
(1.3) 		25.9
(-1.1) 		28.5
(-0.7) 		9.8
(-3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176259_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15552
Sequence Type Exemplar sequence
Representative Public ID Y50D4B.3 NCBI
Target Description Y50D4B.3 /REP_DB=WormBase Gene ID /WP=CE26134 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070977(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070977 NCBI Caenorhabditis elegans Y50D4B.3 (Y50D4B.3) mRNA, complete cds. 11/11 A 
SNAP00000024549 ENSEMBL cdna:SNAP chromosome:CEL140:V:1091731:1100545:1 9/11 None
Y50D4B.3 ENSEMBL cdna:known chromosome:CEL140:V:1095192:1098451:1 gene:Y50D4B.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000024552 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1095689-1098451(+) 91.33 93.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4B.3
Entrez Gene 178608 Entrez gene
SwissProt Q966B0 EMBL-EBI
WormBase CE26134 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84611 Hypothetical protein Y50D4B.3 [Caenorhabditis elegans] ref|NP_503378.1| Y50D4B.3 [Caenorhabditis elegans] 0.0
blast AAK84612 Hypothetical protein Y50D4B.4 [Caenorhabditis elegans] ref|NP_503377.2| Y50D4B.4 [Caenorhabditis elegans] 1.0E-151
blast CAA21046 Hypothetical protein Y47H9A.1 [Caenorhabditis elegans] emb|CAB03899.2| Hypothetical protein Y47H9A.1 [Caenorhabditis elegans] ref|NP_492963.1| Y47H9A.1 [Caenorhabditis elegans] 1.0E-129
blast CAA21046 Hypothetical protein Y47H9A.1 [Caenorhabditis elegans] emb|CAB03899.2| Hypothetical protein Y47H9A.1 [Caenorhabditis elegans] ref|NP_492963.1| Y47H9A.1 [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 8.1E-24
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 6.5E-37
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 5.5E-32
Pfam IPR006588 EMBL-EBI Protein of unknown function PAW 1.4E-37
Sequence
Target Sequence 
>C. ELEGANS:176259_AT
gatgttcggggcttttgagcggtttttcggttaaagcctactggggtgagtttttttcac
gacttcggtgggtttcaacgcaagatcaccttctcaacatttcctctacattccatgacg
acagtggacgggtttttgcactgtccccaaagactggagaaaactatgcaaagttcacgt
ataacttcatcaataatttgtattcacacaccgagccagatgggtcaccagtcagattat
tcgattttaatggcttggaacgagtagaggattatacagagaatactgtattcttacgca
gaaaaaacccaaaggaaaagggaaacattcgatgggcgctcaatttgagaatcgatggaa
aatacgtggaaaaagtagttattggaataattggaattgaagagattgccaatcggggag
gaacggccagagcctgttggaatgttttatacaattgcatcgatattccgattgacggaa
ctttcacattgcaaaataatgttgtcgatctttctttggatatagagctggacgaggata
ttcaaatttttaggaccaaactcgacgatgagggcagtttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTTCGGGGCTTTTGAGCGGTTT 401 409 584 Antisense
TGAGTTTTTTTCACGACTTCGGTGG 489 565 630 Antisense
TCACGACTTCGGTGGGTTTCAACGC 171 599 640 Antisense
TAATTTGTATTCACACACCGAGCCA 464 635 777 Antisense
GAAACATTCGATGGGCGCTCAATTT 360 355 905 Antisense
GAGATTGCCAATCGGGGAGGAACGG 118 397 985 Antisense
GAACGGCCAGAGCCTGTTGGAATGT 308 349 1004 Antisense
ATTGCATCGATATTCCGATTGACGG 672 5 1037 Antisense
GATTGACGGAACTTTCACATTGCAA 144 433 1053 Antisense
ATAATGTTGTCGATCTTTCTTTGGA 288 25 1079 Antisense
AACTCGACGATGAGGGCAGTTTGGA 672 139 1142 Antisense
Sequence Source Affymetrix Proprietary Database