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[176258_at]

Description Y52E8A.2 /REP_DB=WormBase Gene ID /WP=CE25390 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 09      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 250
1 2 3 4 range
A 25.8
(-3)
A 		63.3
(-1.9)
A 		101.9
(-0.8)
A 		229.4
(3)
P 		204
B 10.1
(-3)
A 		61
(-1.7)
A 		92.1
(-1)
A 		250.8
(3)
P 		241
C 71.9
(-1.9)
A 		78.5
(-1.7)
A 		28
(-3)
A 		260.2
(3)
P 		232
avr 35.9
(-3) 		67.6
(-2.1) 		74
(-1.9) 		246.8
(3) 		211

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 955
1 2 3 4 5 6 range
A 16.4
(-3)
A 		68.1
(-2.4)
A 		90
(-2.2)
A 		171
(-1.3)
A 		321.6
(0.3)
P 		568.6
(3)
P 		552
B 24.9
(-3)
A 		31.7
(-3)
A 		52
(-2.8)
A 		121.9
(-2.4)
A 		444.3
(-0.3)
P 		971.6
(3)
P 		947
C 35.9
(-3)
A 		63
(-2.6)
A 		66.3
(-2.6)
A 		125.4
(-1.8)
A 		354.7
(1.2)
P 		489.5
(3)
P 		454
avr 25.7
(-3) 		54.3
(-2.7) 		69.4
(-2.6) 		139.4
(-2) 		373.5
(0.2) 		676.6
(3) 		651
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 9.4
(-3)
A 		13.7
(-1.9)
A 		26.6
(1.3)
A 		33.6
(3)
P 		24
B 3.2
(-3)
A 		15.1
(-1.6)
A 		21.3
(-0.8)
A 		53.4
(3)
P 		50
C 23.8
(-0.5)
A 		22.6
(-0.7)
A 		9.7
(-3)
A 		43.9
(3)
P 		34
avr 12.1
(-3) 		17.1
(-2) 		19.2
(-1.6) 		43.6
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 359
1 2 3 4 5 6 range
A 3
(-3)
A 		24.2
(-2.1)
A 		19.6
(-2.3)
A 		43.4
(-1.2)
A 		97.1
(1.2)
P 		138.4
(3)
P 		135
B 8.7
(-3)
A 		10.5
(-3)
A 		12.6
(-2.9)
A 		32.3
(-2.6)
A 		180.5
(-0.1)
P 		362.3
(3)
P 		354
C 13.3
(-3)
A 		19.2
(-2.8)
A 		16.9
(-2.9)
A 		25.2
(-2.5)
A 		114.8
(1)
P 		163.9
(3)
P 		151
avr 8.3
(-3) 		18
(-2.7) 		16.4
(-2.8) 		33.6
(-2.3) 		130.8
(0.4) 		221.5
(3) 		213
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176258_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5571
Sequence Type Exemplar sequence
Representative Public ID Y52E8A.2 NCBI
Target Description Y52E8A.2 /REP_DB=WormBase Gene ID /WP=CE25390 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062359(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062359 NCBI Caenorhabditis elegans Y52E8A.2 (Y52E8A.2) mRNA, complete cds. 11/11 None
GENEFINDER00000040251 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4320847:4321527:1 11/11 None
Y52E8A.2 ENSEMBL cdna:known chromosome:CEL140:II:4320842:4321545:1 gene:Y52E8A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4320845-4321526(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y52E8A.2
Entrez Gene 173765 Entrez gene
SwissProt Q9N3H6 EMBL-EBI
WormBase CE25390 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59525 Hypothetical protein Y52E8A.2 [Caenorhabditis elegans] ref|NP_494760.1| Y52E8A.2 [Caenorhabditis elegans] 8.0E-96
blast CAE62272 Hypothetical protein CBG06331 [Caenorhabditis briggsae] 2.0E-25
blast AAC69348 Hypothetical protein Y73C8C.8 [Caenorhabditis elegans] ref|NP_503853.1| Y73C8C.8 [Caenorhabditis elegans] 2.0E-24
Sequence
Target Sequence 
>C. ELEGANS:176258_AT
atccaatgcgacgagccgcggaacttgtcgccagactatcggcagatgaagttacgatgc
gtgccgcgaaatacgagctgatcaagttcaatcgagcattccagaatttcatgctagtgt
tggatgagaatattgaagagattctgatgtcaaacgacaattctactcgaattcagctca
agaagcttccggaatttccatccttctcggattcgtttatgcaagaatatgaaaaagctc
ttctgaatggcgcagagaagctcaaagcttcaacctccagcgaaaaaggatccatcccgt
cagtctccttttcttcatcaatttcaagctctagcgccgagctatcagatgctcgatgcc
ttatctgtctggacgacgttatttcgactgaaaaaactatcaaatgcaattcgtgtagac
gtcggtttcatctcgattgcgcttcccaatggttcaaggaaaagagcgtttgtccagctt
gtaatcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCAATGCGACGAGCCGCGGAACT 565 39 68 Antisense
GGAACTTGTCGCCAGACTATCGGCA 330 529 87 Antisense
GATGCGTGCCGCGAAATACGAGCTG 643 413 123 Antisense
GAAGCTTCCGGAATTTCCATCCTTC 27 341 249 Antisense
TCCTTCTCGGATTCGTTTATGCAAG 1 593 268 Antisense
GCTCTAGCGCCGAGCTATCAGATGC 23 299 395 Antisense
GCTATCAGATGCTCGATGCCTTATC 570 303 408 Antisense
ATGCCTTATCTGTCTGGACGACGTT 188 41 423 Antisense
TAGACGTCGGTTTCATCTCGATTGC 205 651 483 Antisense
ATTGCGCTTCCCAATGGTTCAAGGA 630 3 503 Antisense
GAGCGTTTGTCCAGCTTGTAATCGA 559 385 531 Antisense
Sequence Source Affymetrix Proprietary Database