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[176255_s_at]

Description Y71H2AM.M /REP_DB=WormBase Gene ID /WP=CE26267 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 14      4grp_high_8_8 > 34      6grp_highest_nonorm > 70      6grp_total > 04      4grp_total > 38      6grp_highest_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 227
1 2 3 4 range
A 428.8
(3)
P 		272.5
(-1.7)
P 		229.2
(-3)
P 		283.5
(-1.4)
A 		200
B 456.4
(3)
P 		346.3
(0.1)
P 		255.1
(-2.3)
P 		230.3
(-3)
P 		226
C 405.9
(3)
P 		368.6
(1.6)
P 		242.3
(-3)
P 		331.9
(0.3)
P 		164
avr 430.4
(3) 		329.1
(-0.2) 		242.2
(-3) 		281.9
(-1.7) 		188

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 240
1 2 3 4 5 6 range
A 376.9
(3)
P 		352.1
(2.2)
P 		229.6
(-1.6)
P 		266.5
(-0.5)
P 		257.3
(-0.8)
P 		185.8
(-3)
P 		191
B 393.5
(3)
P 		276.1
(0.1)
P 		298.1
(0.6)
P 		284.1
(0.3)
P 		247.1
(-0.7)
P 		153.1
(-3)
P 		240
C 312.1
(1.9)
P 		343.6
(3)
P 		237.3
(-0.6)
P 		240.5
(-0.5)
P 		195.8
(-2)
P 		167.1
(-3)
P 		177
avr 360.8
(3) 		323.9
(1.8) 		255
(-0.3) 		263.7
(-0) 		233.4
(-1) 		168.7
(-3) 		192
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 156.1
(3)
P 		59.1
(-2.1)
P 		59.9
(-2)
P 		41.5
(-3)
A 		115
B 145.5
(3)
P 		85.5
(-0.7)
P 		59.1
(-2.4)
P 		49.1
(-3)
P 		96
C 134.4
(3)
P 		106
(0.8)
P 		84.4
(-0.8)
P 		56
(-3)
P 		78
avr 145.3
(3) 		83.5
(-0.8) 		67.8
(-1.8) 		48.9
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 69.5
(-1.2)
P 		125.2
(3)
P 		50
(-2.6)
P 		67.6
(-1.3)
P 		77.6
(-0.6)
P 		45.2
(-3)
P 		80
B 136.9
(3)
P 		91.9
(-0.4)
P 		72.4
(-1.8)
P 		75.3
(-1.6)
P 		100.4
(0.3)
P 		57.1
(-3)
P 		80
C 115.7
(3)
P 		104.8
(2)
P 		60.4
(-1.9)
P 		48.4
(-3)
P 		63.4
(-1.7)
P 		55.9
(-2.3)
P 		67
avr 107.4
(3) 		107.3
(3) 		60.9
(-2.1) 		63.8
(-1.8) 		80.5
(0) 		52.7
(-3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176255_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18965
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.M NCBI
Target Description Y71H2AM.M /REP_DB=WormBase Gene ID /WP=CE26267 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065204(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065204 NCBI Caenorhabditis elegans Y71H2AM.10 (Y71H2AM.10) mRNA, complete cds. 11/11 None
GENEFINDER00000014477 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2707565:2715289:-1 11/11 None
SNAP00000014435 ENSEMBL cdna:SNAP chromosome:CEL140:III:2707850:2711199:-1 11/11 None
Y71H2AM.10 ENSEMBL cdna:known chromosome:CEL140:III:2707727:2714916:-1 gene:Y71H2AM.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2707849-2714916(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.10
Entrez Gene 175386 Entrez gene
SwissProt Q9BL47 EMBL-EBI
WormBase CE29141 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29969 Hypothetical protein Y71H2AM.10 [Caenorhabditis elegans] ref|NP_497605.1| Y71H2AM.10 [Caenorhabditis elegans] 0.0
blast CAE65001 Hypothetical protein CBG09839 [Caenorhabditis briggsae] 0.0
blast CAE65001 Hypothetical protein CBG09839 [Caenorhabditis briggsae] 1.0E-159
blast AAF46133 CG14446-PA [Drosophila melanogaster] ref|NP_572300.1| CG14446-PA [Drosophila melanogaster] 1.0E-34
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497605.1 1 691-713
Y71H2AM.10 1 691-713
GENEFINDER00000014477 1 785-807
SNAP00000014435 1 201-223
Sequence
Target Sequence 
>C. ELEGANS:176255_S_AT
gccactgacctggattcaagcttctcaactaccatagcaccttccgattcacttccatcc
atcccatttcacatttttgtgctcacaattatcggattaattatacttttcttatttatt
tcatttgtacggagatccgcagcgttcaaggggtatgagcaactcgtggtaccatttttc
tcgcggttatcctcatcgtcgggctcaaattccagacaggaagagacaaatgaatgggta
tggctcagtcagccacagccgccatcctccactataagttctgggtattctggaaacaag
tcgaccgcagaacggcaatcgtcaaatggtgacgacccgtcaagaacgagcatctcgtat
cacggttctgaaatttcggttttcattgcaccgtcgcagggaaatgtcgtggtaaaccaa
tcctcccgtcaccctcgctacactctcgtcgactcgaattccgatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCACTGACCTGGATTCAAGCTTCT 502 279 2086 Antisense
CATCCATCCCATTTCACATTTTTGT 369 211 2141 Antisense
AGATCCGCAGCGTTCAAGGGGTATG 104 67 2218 Antisense
TGGTACCATTTTTCTCGCGGTTATC 458 545 2252 Antisense
CTCATCGTCGGGCTCAAATTCCAGA 279 229 2277 Antisense
GTCGACCGCAGAACGGCAATCGTCA 69 473 2385 Antisense
CGACCCGTCAAGAACGAGCATCTCG 455 249 2418 Antisense
GAGCATCTCGTATCACGGTTCTGAA 457 387 2433 Antisense
TTTCGGTTTTCATTGCACCGTCGCA 270 671 2459 Antisense
ATGTCGTGGTAAACCAATCCTCCCG 152 47 2489 Antisense
CTCTCGTCGACTCGAATTCCGATCA 291 231 2528 Antisense
Sequence Source Affymetrix Proprietary Database