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[176251_at]

Description Y82E9BL.D /REP_DB=WormBase Gene ID /WP=CE23420 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 87      4grp_total > 09      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 4.6
(-1.6)
A 		11.2
(3)
A 		7.9
(0.7)
A 		2.5
(-3)
A 		9
B 6.2
(3)
A 		2.5
(-3)
A 		3.2
(-1.9)
A 		3.6
(-1.2)
A 		4
C 3.5
(-3)
A 		4.8
(-2.8)
A 		8.9
(-2.1)
A 		40.5
(3)
A 		37
avr 4.8
(-3) 		6.2
(-2.2) 		6.7
(-1.9) 		15.5
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 7.2
(-0.2)
A 		2.8
(-2.8)
A 		4.8
(-1.6)
A 		2.4
(-3)
A 		12.8
(3)
A 		5.4
(-1.3)
A 		10
B 3
(-2.7)
A 		2
(-3)
A 		3.6
(-2.5)
A 		10.5
(-0.5)
A 		8.7
(-1)
A 		22.6
(3)
A 		21
C 4.1
(-0.6)
A 		8.2
(3)
A 		1.8
(-2.6)
A 		2.6
(-1.9)
A 		1.4
(-3)
A 		7.5
(2.4)
A 		7
avr 4.8
(-2) 		4.3
(-2.4) 		3.4
(-3) 		5.2
(-1.7) 		7.6
(-0) 		11.8
(3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 1.7
(0.9)
A 		2.4
(3)
A 		2.1
(2.1)
A 		0.4
(-3)
A 		2
B 2
(3)
A 		0.6
(-3)
A 		0.7
(-2.6)
A 		0.8
(-2.1)
A 		1
C 1.2
(-3)
A 		1.4
(-2.8)
A 		3.1
(-1)
A 		6.8
(3)
A 		6
avr 1.6
(-2.5) 		1.5
(-3) 		2
(-0.5) 		2.7
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.3
(-1.7)
A 		1
(-2.3)
A 		1
(-2.3)
A 		0.6
(-3)
A 		3.9
(3)
A 		1.3
(-1.7)
A 		3
B 1.1
(-2.7)
A 		0.7
(-3)
A 		0.9
(-2.8)
A 		2.8
(-1.4)
A 		3.5
(-0.8)
A 		8.4
(3)
A 		8
C 1.5
(0)
A 		2.5
(3)
A 		0.5
(-3)
A 		0.5
(-3)
A 		0.5
(-3)
A 		2.5
(3)
A 		2
avr 1.3
(-2.1) 		1.4
(-1.9) 		0.8
(-3) 		1.3
(-2.1) 		2.6
(0.3) 		4.1
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176251_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19034
Sequence Type Exemplar sequence
Representative Public ID Y82E9BL.D NCBI
Target Description Y82E9BL.D /REP_DB=WormBase Gene ID /WP=CE23420 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064988(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064988 NCBI Caenorhabditis elegans Y82E9BL.5 (Y82E9BL.5) mRNA, complete cds. 10/11 None
GENEFINDER00000041785 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1345341:1350826:-1 10/11 None
SNAP00000041773 ENSEMBL cdna:SNAP chromosome:CEL140:III:1346305:1349306:-1 10/11 None
Y82E9BL.5 ENSEMBL cdna:known chromosome:CEL140:III:1346305:1347118:-1 gene:Y82E9BL.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1346304-1349306(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y82E9BL.5
Entrez Gene 190754 Entrez gene
SwissProt Q95XB3 EMBL-EBI
WormBase CE27547 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK66032 Hypothetical protein Y82E9BL.5 [Caenorhabditis elegans] ref|NP_497389.1| Y82E9BL.5 [Caenorhabditis elegans] 1.0E-131
blast AAK66032 Hypothetical protein Y82E9BL.5 [Caenorhabditis elegans] ref|NP_497389.1| Y82E9BL.5 [Caenorhabditis elegans] 1.0E-130
blast AAK66031 Hypothetical protein Y82E9BL.4 [Caenorhabditis elegans] ref|NP_497390.1| Y82E9BL.4 [Caenorhabditis elegans] 1.0E-102
blast AAG37970 Hypothetical protein Y82E9BL.3 [Caenorhabditis elegans] ref|NP_497391.1| Y82E9BL.3 [Caenorhabditis elegans] 5.0E-90
blast AAK66033 Hypothetical protein Y82E9BL.6 [Caenorhabditis elegans] ref|NP_497388.1| Y82E9BL.6 [Caenorhabditis elegans] 7.0E-87
blast AAK66033 Hypothetical protein Y82E9BL.6 [Caenorhabditis elegans] ref|NP_497388.1| Y82E9BL.6 [Caenorhabditis elegans] 2.0E-86
blast AAG37970 Hypothetical protein Y82E9BL.3 [Caenorhabditis elegans] ref|NP_497391.1| Y82E9BL.3 [Caenorhabditis elegans] 2.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.5E-7
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.4E-13
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.3E-7
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.9E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497389.1 6 7-29,39-57,70-92,102-124,146-168,183-205
Y82E9BL.5 6 7-29,39-57,70-92,102-124,146-168,183-205
GENEFINDER00000041785 11 15-32,52-74,84-103,124-146,156-178,452-474,484-501,514-536,546-568,590-612,627-649
SNAP00000041773 6 188-210,220-237,250-272,282-304,326-348,363-385
Sequence
Target Sequence 
>C. ELEGANS:176251_AT
attctcgtttcaattgcgttatcttgtaaactagttgataagagaaaaaaccaacagtct
atcgaaaacttctgctggcatcggatccttattgcaggcgcagtggtctgtttgaatgtt
ttatttccggtggctatgattaccatgtttcttggaggatggatgaatacccttgcctct
atattgatctatctcctactgctttgcccacctatcagtcttgcaaatgttctgttagaa
tctgaatcaccaatcaagtacacttttccaaaaaccacaaagctaaccaaccttcaaaag
gtttccatcatcctattcttcaccattatggaagtatactatatctacttgtgcacttgg
acacatcattctcaaaacgagtataatattatgaacaaattatggccagaaacagtcatt
gtggcctgctatgcacacaactcggaaaaaatgaatttatcacgtttgagacctgatgat
ggattagaacttattaagatcagaacttggaaccctgcgact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCTCGTTTCAATTGCGTTATCTT 501 9 964 Antisense
GTCTATCGAAAACTTCTGCTGGCAT 287 469 1020 Antisense
GCATCGGATCCTTATTGCAGGCGCA 675 309 1041 Antisense
TGCAGGCGCAGTGGTCTGTTTGAAT 129 579 1056 Antisense
GTGGCTATGATTACCATGTTTCTTG 34 493 1093 Antisense
AATACCCTTGCCTCTATATTGATCT 389 171 1129 Antisense
ATATTGATCTATCTCCTACTGCTTT 413 19 1144 Antisense
TGCCCACCTATCAGTCTTGCAAATG 229 585 1168 Antisense
GTGCACTTGGACACATCATTCTCAA 245 481 1314 Antisense
ACAGTCATTGTGGCCTGCTATGCAC 665 107 1375 Antisense
GATCAGAACTTGGAACCCTGCGACT 253 421 1461 Antisense
Sequence Source Affymetrix Proprietary Database