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[176244_at]

Description Y75B7AL.A /REP_DB=WormBase Gene ID /WP=CE26301 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 76      4grp_total > 12      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 113.7
(0.3)
A 		95.4
(-1.1)
A 		70.6
(-3)
A 		148.7
(3)
A 		78
B 100.4
(-0.4)
A 		112
(0.6)
A 		69.5
(-3)
A 		141.1
(3)
A 		72
C 126.8
(-0.8)
A 		81.8
(-3)
A 		103.6
(-2)
A 		206.6
(3)
A 		125
avr 113.6
(-0.7) 		96.4
(-1.9) 		81.2
(-3) 		165.5
(3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 68.8
(2.5)
A 		27.8
(-1.1)
A 		43.8
(0.3)
A 		6.5
(-3)
A 		74.3
(3)
A 		72.3
(2.8)
A 		68
B 60.8
(-0.2)
A 		16.9
(-2.6)
A 		9
(-3)
A 		53.5
(-0.6)
A 		35.5
(-1.6)
A 		118.9
(3)
P 		110
C 17.8
(-3)
A 		48.7
(-1.1)
A 		68.1
(0.2)
A 		113.5
(3)
A 		27.2
(-2.4)
A 		92.1
(1.7)
P 		96
avr 49.1
(-1.3) 		31.1
(-3) 		40.3
(-2.1) 		57.8
(-0.5) 		45.7
(-1.6) 		94.4
(3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 41.4
(3)
A 		20.7
(-2.4)
A 		18.5
(-3)
A 		21.8
(-2.1)
A 		23
B 32
(3)
A 		27.7
(1.4)
A 		16.1
(-3)
A 		30.1
(2.3)
A 		16
C 42
(3)
A 		23.5
(-3)
A 		36.1
(1.1)
A 		34.8
(0.7)
A 		19
avr 38.5
(3) 		24
(-2.8) 		23.6
(-3) 		28.9
(-0.9) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 12.7
(0.2)
A 		9.9
(-0.6)
A 		9.5
(-0.7)
A 		1.6
(-3)
A 		22.4
(3)
A 		17.6
(1.6)
A 		21
B 21.2
(-0.3)
A 		5.6
(-2.5)
A 		2.2
(-3)
A 		14.2
(-1.3)
A 		14.4
(-1.3)
A 		44.3
(3)
P 		42
C 6.6
(-3)
A 		14.9
(-0.9)
A 		17.3
(-0.3)
A 		22.8
(1)
A 		8.8
(-2.5)
A 		30.8
(3)
P 		24
avr 13.5
(-1.9) 		10.1
(-2.9) 		9.7
(-3) 		12.9
(-2.1) 		15.2
(-1.4) 		30.9
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176244_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19505
Sequence Type Exemplar sequence
Representative Public ID Y75B7AL.A NCBI
Target Description Y75B7AL.A /REP_DB=WormBase Gene ID /WP=CE26301 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071006(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071006 NCBI Caenorhabditis elegans Y75B7AL.4 (Y75B7AL.4) mRNA, complete cds. 11/11 None
SNAP00000018279 ENSEMBL cdna:SNAP chromosome:CEL140:V:1369621:1383068:-1 9/11 None
GENEFINDER00000018283 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1374627:1383924:-1 9/11 None
Y75B7AL.4 ENSEMBL cdna:known chromosome:CEL140:V:1373418:1383068:-1 gene:Y75B7AL.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000018280 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1373417-1383234(-) 96.89 99.94
Public Domain and Genome References
Gene Title
Ensembl Y75B7AL.4
Entrez Gene 178629 Entrez gene
SwissProt Q965S4 EMBL-EBI
WormBase CE38126 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68606 Hypothetical protein Y75B7AL.4 [Caenorhabditis elegans] ref|NP_503407.3| Y75B7AL.4 [Caenorhabditis elegans] 0.0
blast NP_504503 K09H11.3 [Caenorhabditis elegans] gb|AAB52263.1| Hypothetical protein K09H11.3 [Caenorhabditis elegans] 0.0
blast CAE62351 Hypothetical protein CBG06428 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000198 EMBL-EBI RhoGAP domain 6.3E-35
Pfam IPR000198 EMBL-EBI RhoGAP domain 6.3E-35
Sequence
Target Sequence 
>C. ELEGANS:176244_AT
cgacaatggattcgagcttcctgccactgcttccactgctgctgctgctgctggggtacc
agatgagacacagttcaagcttccgcaattgccgatgccgccgaagaaaaaagtgtctcc
gctgcttgcccggcaagccactcactcatcaaagtcgacgacgctttcggtgccgaatcg
agcgaatcttcgagaaacgaagagcatcgatgaaactccgacagcattgagatcggcacc
accagcagctcaaggatcttcgtcgaatcagctgtcgccaagatcatccactgattactt
cgagtttggaaaatacacaaatgcgttgagatcggcgactgatgagtttgaaatcgaaca
ttcgcaacgcgtcgaaacatcgatgagcggtcgattgtctgcaatgtcacaatcatcact
ccgaagcagcaatgatacacaccaatcatcgtcggatcgtttttcggatgtctcggatct
tcggaatgccgcgttctctgaatccacctcggactcggcgctcagcactccaactgccac
tccaacaacttcacgggtctcgacgaacgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACAATGGATTCGAGCTTCCTGCC 177 253 2883 Antisense
CAATTGCCGATGCCGCCGAAGAAAA 282 179 2968 Antisense
AGAAAAAAGTGTCTCCGCTGCTTGC 239 73 2987 Antisense
GGTGCCGAATCGAGCGAATCTTCGA 65 503 3051 Antisense
GCATCGATGAAACTCCGACAGCATT 620 309 3086 Antisense
CGACAGCATTGAGATCGGCACCACC 13 253 3101 Antisense
CTTCGTCGAATCAGCTGTCGCCAAG 200 219 3140 Antisense
GTCGCCAAGATCATCCACTGATTAC 534 475 3156 Antisense
CAAATGCGTTGAGATCGGCGACTGA 174 193 3200 Antisense
GATACACACCAATCATCGTCGGATC 264 423 3316 Antisense
AACTTCACGGGTCTCGACGAACGAT 501 101 3429 Antisense
Sequence Source Affymetrix Proprietary Database