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[176243_at]

Description Y105E8B.SS /REP_DB=WormBase Gene ID /WP=CE23207 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 49      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 85      6grp_total > 49      4grp_total > 61      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 449
1 2 3 4 range
A 749.6
(0.8)
P 		713.9
(0.1)
P 		866.6
(3)
P 		553.4
(-3)
P 		313
B 728
(3)
P 		603.7
(-3)
P 		663.8
(-0.1)
P 		612.2
(-2.6)
P 		124
C 812.9
(-0.2)
P 		681.2
(-2.5)
P 		1002.2
(3)
P 		650.3
(-3)
P 		352
avr 763.5
(1) 		666.3
(-1.5) 		844.2
(3) 		605.3
(-3) 		239

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 538
1 2 3 4 5 6 range
A 933.1
(3)
P 		789.9
(0.6)
P 		575.4
(-3)
P 		761.1
(0.1)
P 		754.4
(0)
P 		906.5
(2.6)
P 		358
B 839.7
(1.5)
P 		712.1
(0)
P 		439.1
(-3)
P 		740
(0.4)
P 		736.3
(0.3)
P 		976.9
(3)
P 		538
C 971.7
(3)
P 		673.4
(-2.1)
P 		621
(-3)
P 		736.6
(-1)
P 		803.7
(0.1)
P 		951.3
(2.7)
P 		351
avr 914.8
(2.5) 		725.1
(-0.3) 		545.2
(-3) 		745.9
(0) 		764.8
(0.3) 		944.9
(3) 		400
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 268
1 2 3 4 range
A 272.9
(3)
P 		155
(-0.7)
P 		226.5
(1.5)
P 		81
(-3)
P 		192
B 232.1
(3)
P 		149.1
(-1.9)
P 		153.8
(-1.6)
P 		130.5
(-3)
P 		102
C 269.1
(1)
P 		195.8
(-0.8)
P 		349.3
(3)
P 		109.7
(-3)
P 		240
avr 258
(3) 		166.6
(-0.6) 		243.2
(2.4) 		107.1
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 258
1 2 3 4 5 6 range
A 172
(-1.2)
P 		280.9
(3)
P 		125.3
(-3)
P 		193
(-0.4)
P 		227.6
(0.9)
P 		220.7
(0.7)
P 		156
B 292.2
(1.3)
P 		237
(0)
P 		106.6
(-3)
P 		196
(-0.9)
P 		299.1
(1.5)
P 		364.3
(3)
P 		258
C 360.2
(3)
P 		205.5
(-1.4)
P 		158.1
(-2.7)
P 		148.1
(-3)
P 		260.1
(0.2)
P 		318.5
(1.8)
P 		212
avr 274.8
(2.1) 		241.1
(0.9) 		130
(-3) 		179
(-1.3) 		262.3
(1.6) 		301.2
(3) 		171
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176243_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18812
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.SS NCBI
Target Description Y105E8B.SS /REP_DB=WormBase Gene ID /WP=CE23207 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026533(11), NM_001026534(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026533 NCBI Caenorhabditis elegans Endoplasmic Reticulum Oxidase family member (ero-1) (ero-1) mRNA, complete cds. 11/11 None
NM_001026534 NCBI Caenorhabditis elegans Endoplasmic Reticulum Oxidase family member (ero-1) (ero-1) mRNA, complete cds. 11/11 None
GENEFINDER00000011745 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14672569:14682726:1 11/11 None
SNAP00000011739 ENSEMBL cdna:SNAP chromosome:CEL140:I:14676432:14682726:1 11/11 None
Y105E8B.8a ENSEMBL cdna:known chromosome:CEL140:I:14672567:14682908:1 gene:Y105E8B.8 11/11 None
Y105E8B.8b ENSEMBL cdna:known chromosome:CEL140:I:14672567:14682726:1 gene:Y105E8B.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14679329-14682657(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y105E8B.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC70110 Hypothetical protein Y105E8B.8a [Caenorhabditis elegans] ref|NP_001021704.1| Endoplasmic Reticulum Oxidase family member (ero-1) [Caenorhabditis elegans] sp|Q7YTU4|ERO1_CAEEL Endoplasmic oxidoreductin 1 precursor 0.0
blast CAD92388 Hypothetical protein Y105E8B.8b [Caenorhabditis elegans] ref|NP_001021705.1| Endoplasmic Reticulum Oxidase family member (ero-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 1.0E-126
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 5.6E-113
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 1.7E-17
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 5.6E-113
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 1.0E-70
Sequence
Target Sequence 
>C. ELEGANS:176243_AT
ggaccacaacgtcttcgaaatgtctatttcatctatttactcgaacttcgtgcactttta
aaagctgctccataccttcaaaatgagctattctacactggaaatgacgtggaagacgct
gaaactcgaaaagccgtcgaagatcttttagaagaaattcgtgcgtatccaaatcatttt
gacgaatcggaaatgttcactggtgttgaatcacatgcacgggctctccgagaggaattc
cgatcgcattttgtgaatatctcgagaattatggattgtgttgagtgcgataaatgtcgg
ctttggggaaaagttcagacacacggaatgggaacggcgctcaaaattcttttttcggat
ttaccacattcacattataaacaggactcgtcgaagttccagttgactagaaacgaagtg
gtcgcccttttgcaaagtttcggtcgttactcgtcaagtat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACCACAACGTCTTCGAAATGTCT 564 521 202 Antisense
ATTTACTCGAACTTCGTGCACTTTT 673 15 236 Antisense
TAAAAGCTGCTCCATACCTTCAAAA 9 641 260 Antisense
GAAATTCGTGCGTATCCAAATCATT 479 363 355 Antisense
TGTTGAATCACATGCACGGGCTCTC 274 563 405 Antisense
GGCTCTCCGAGAGGAATTCCGATCG 209 539 423 Antisense
ACACACGGAATGGGAACGGCGCTCA 147 111 520 Antisense
TTTTCGGATTTACCACATTCACATT 536 677 553 Antisense
GGACTCGTCGAAGTTCCAGTTGACT 554 521 585 Antisense
TCGCCCTTTTGCAAAGTTTCGGTCG 566 597 623 Antisense
GTTTCGGTCGTTACTCGTCAAGTAT 577 447 638 Antisense
Sequence Source Affymetrix Proprietary Database