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[176240_s_at]

Description Y71H2AM.A /REP_DB=WormBase Gene ID /WP=CE22929 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 03      4grp_highest_8_8 > 18      4grp_high_8_8 > 60      6grp_highest_nonorm > 23      6grp_total > 35      4grp_total > 60      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1604
1 2 3 4 range
A 2944.7
(0.6)
P 		2641.6
(-0.5)
P 		3589.9
(3)
P 		1985.8
(-3)
P 		1604
B 2948.5
(1.4)
P 		2873.1
(1)
P 		3238.1
(3)
P 		2166.7
(-3)
P 		1071
C 2477.4
(-1.3)
P 		2985.3
(1.1)
P 		3386.6
(3)
P 		2131
(-3)
P 		1256
avr 2790.2
(0.2) 		2833.3
(0.4) 		3404.9
(3) 		2094.5
(-3) 		1310

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1404
1 2 3 4 5 6 range
A 2518.6
(3)
P 		2490.8
(2.8)
P 		1871.1
(-1.4)
P 		1879.1
(-1.3)
P 		1634.7
(-3)
P 		1962
(-0.8)
P 		884
B 2598.5
(2)
P 		2721.2
(2.6)
P 		2803.1
(3)
P 		1887.4
(-1.6)
P 		1598.9
(-3)
P 		1968
(-1.2)
P 		1204
C 2871
(3)
P 		2576.7
(1.7)
P 		2539.4
(1.6)
P 		1725.3
(-1.9)
P 		1467.1
(-3)
P 		1870.4
(-1.3)
P 		1404
avr 2662.7
(3) 		2596.2
(2.6) 		2404.5
(1.6) 		1830.6
(-1.6) 		1566.9
(-3) 		1933.5
(-1) 		1096
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 890
1 2 3 4 range
A 1072.2
(3)
P 		573.4
(-0.8)
P 		938.5
(2)
P 		290.7
(-3)
P 		782
B 940.3
(3)
P 		709.6
(0.1)
P 		750.3
(0.6)
P 		461.7
(-3)
P 		479
C 820.1
(0.4)
P 		858.3
(0.6)
P 		1180.3
(3)
P 		359.3
(-3)
P 		821
avr 944.2
(2.9) 		713.8
(0.5) 		956.4
(3) 		370.6
(-3) 		586

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 717
1 2 3 4 5 6 range
A 464.3
(-2.3)
P 		885.9
(3)
P 		407.5
(-3)
P 		476.6
(-2.1)
P 		493.3
(-1.9)
P 		477.7
(-2.1)
P 		478
B 904.1
(3)
P 		905.8
(3)
P 		680.7
(-0.3)
P 		499.9
(-3)
P 		649.6
(-0.8)
P 		733.9
(0.5)
P 		406
C 1064.2
(3)
P 		786.2
(0.7)
P 		646.3
(-0.5)
P 		347
(-3)
P 		474.7
(-1.9)
P 		626.1
(-0.7)
P 		717
avr 810.9
(2.3) 		859.3
(3) 		578.2
(-1) 		441.2
(-3) 		539.2
(-1.6) 		612.6
(-0.5) 		418
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176240_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18967
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.A NCBI
Target Description Y71H2AM.A /REP_DB=WormBase Gene ID /WP=CE22929 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065222(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065222 NCBI Caenorhabditis elegans Y71H2AM.23 (Y71H2AM.23) mRNA, complete cds. 11/11 A 
SNAP00000014456 ENSEMBL cdna:SNAP chromosome:CEL140:III:2805672:2814775:-1 11/11 None
GENEFINDER00000014474 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2812312:2815285:-1 11/11 None
Y71H2AM.23.2 ENSEMBL cdna:known chromosome:CEL140:III:2811679:2814961:-1 gene:Y71H2AM.23 11/11 None
Y71H2AM.23.1 ENSEMBL cdna:known chromosome:CEL140:III:2812044:2814959:-1 gene:Y71H2AM.23 11/11 None
Y71H2AM.23.3 ENSEMBL cdna:known chromosome:CEL140:III:2812133:2814959:-1 gene:Y71H2AM.23 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2812311-2815264(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.23
Entrez Gene 175398 Entrez gene
SwissProt Q19072 EMBL-EBI
WormBase CE27322 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18713_AT elongation factor Tu, putative / EF-Tu, putative at
ATH1-121501:255455_AT elongation factor Tu, putative / EF-Tu, putative at
CANINE:1583725_AT similar to Tu translation elongation factor, mitochondrial cfa
CANINE_2:CFA.10170.1.A1_AT similar to Tu translation elongation factor, mitochondrial cfa
CANINE_2:CFA.10170.1.A1_S_AT similar to Tu translation elongation factor, mitochondrial cfa
CANINE_2:CFAAFFX.26342.1.S1_AT similar to Tu translation elongation factor, mitochondrial cfa
DROSOPHILA_2:1623588_AT Elongation factor Tu mitochondrial dm
DROSGENOME1:142510_AT Elongation factor Tu mitochondrial dm
HG-U133_PLUS_2:201113_AT Tu translation elongation factor, mitochondrial hs
HG-U133A_2:201113_AT Tu translation elongation factor, mitochondrial hs
HG-U133A:201113_AT Tu translation elongation factor, mitochondrial hs
HG-FOCUS:201113_AT Tu translation elongation factor, mitochondrial hs
HUGENEFL:S75463_AT Tu translation elongation factor, mitochondrial hs
U133_X3P:G4507732_3P_AT Tu translation elongation factor, mitochondrial hs
U133_X3P:HS.171379.0.A1_3P_AT Tu translation elongation factor, mitochondrial hs
HG-U133_PLUS_2:238190_AT Tu translation elongation factor, mitochondrial hs
HG-U133B:238190_AT Tu translation elongation factor, mitochondrial hs
HG-U95AV2:39867_AT Tu translation elongation factor, mitochondrial hs
HG-U95D:92041_AT Tu translation elongation factor, mitochondrial hs
MU11KSUBB:MSA.27354.0_R_AT Tu translation elongation factor, mitochondrial mm
MU11KSUBB:MSA.27354.0_F_AT Tu translation elongation factor, mitochondrial mm
MU11KSUBB:MSA.27354.0_I_AT Tu translation elongation factor, mitochondrial mm
MG-U74AV2:96626_AT Tu translation elongation factor, mitochondrial mm
MG-U74AV2:161120_R_AT Tu translation elongation factor, mitochondrial mm
MOE430A:1449672_S_AT Tu translation elongation factor, mitochondrial mm
MOUSE430A_2:1449672_S_AT Tu translation elongation factor, mitochondrial mm
MOUSE430_2:1449672_S_AT Tu translation elongation factor, mitochondrial mm
MOE430B:1447780_X_AT Tu translation elongation factor, mitochondrial mm
MOUSE430_2:1447780_X_AT Tu translation elongation factor, mitochondrial mm
MOE430A:1449673_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MOUSE430A_2:1449673_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MOUSE430_2:1449673_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MU11KSUBB:MSA.3871.0_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MU19KSUBA:TC22797_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MU19KSUBC:TC39794_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
RG-U34A:RC_AA866234_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAE230A:1371962_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAT230_2:1371962_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAE230B:1395647_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAT230_2:1395647_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAE230B:1395719_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAT230_2:1395719_AT Tu translation elongation factor, mitochondrial (predicted) rn
RG-U34C:RC_AI175980_AT Tu translation elongation factor, mitochondrial (predicted) rn
YEAST_2:1769573_AT Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6414 translational elongation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3746 translation elongation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29979 Hypothetical protein Y71H2AM.23 [Caenorhabditis elegans] ref|NP_497623.1| Y71H2AM.23 [Caenorhabditis elegans] dbj|BAA07491.1| elongation factor Tu homologue precursor [Caenorhabditis elegans] 0.0
blast BAA07492.1 elongation factor Tu homologue precursor [Caenorhabditis elegans] 0.0
blast CAE57516 Hypothetical protein CBG00491 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 4.9E-86
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 4.7E-22
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 7.1E-32
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 4.9E-86
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 4.7E-22
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 7.1E-32
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 4.7E-22
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 4.9E-86
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 7.1E-32
Sequence
Target Sequence 
>C. ELEGANS:176240_S_AT
gaatccttccgtaaaactgtcgatcaagccgagcctggagatcaattgggtgtgcttctt
cgtggtttaggaccaaaagatgttcgtagaggatgtgttttacttccacaaggacacaaa
cataaggtcactgataaggttaaagctcaactttatgtgctcaaagagagcgaaggaggc
gccaagacaccgattgccaactatttcagtgagcacgtcttctcattgacatgggacagt
ggagcaagtgtcagaattattggaaaagactttgtgatgcctggagaatctgcggaagtc
gagctctcactgaattcccaaatgttcatcgagccccaacaaagattcacaattcgtaaa
ggagccaaaacaattggaaccggtgtgttcaccgacgttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCCTTCCGTAAAACTGTCGATC 364 315 1030 Antisense
AAAACTGTCGATCAAGCCGAGCCTG 64 129 1042 Antisense
GCCGAGCCTGGAGATCAATTGGGTG 245 285 1057 Antisense
TGTGCTTCTTCGTGGTTTAGGACCA 14 539 1080 Antisense
GCGAAGGAGGCGCCAAGACACCGAT 519 287 1199 Antisense
GACACCGATTGCCAACTATTTCAGT 601 367 1215 Antisense
GAGCACGTCTTCTCATTGACATGGG 628 389 1240 Antisense
AAAGACTTTGTGATGCCTGGAGAAT 375 123 1294 Antisense
GAGCTCTCACTGAATTCCCAAATGT 46 389 1330 Antisense
GTTCATCGAGCCCCAACAAAGATTC 293 445 1353 Antisense
GAACCGGTGTGTTCACCGACGTTCT 532 305 1406 Antisense
Sequence Source Affymetrix Proprietary Database