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[176237_at]

Description Y105E8B.RR /REP_DB=WormBase Gene ID /WP=CE24101 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster 6grp_total > 90      4grp_total > 40      6grp_cgc > 96      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 121.5
(3)
A 		48.9
(-2)
A 		119.3
(2.8)
A 		34.7
(-3)
A 		87
B 29.6
(-3)
A 		48
(-2.1)
A 		157.4
(3)
A 		95.4
(0.1)
A 		128
C 85.2
(-1.6)
A 		73
(-3)
A 		124.6
(3)
A 		123.4
(2.9)
A 		52
avr 78.8
(-1.3) 		56.6
(-3) 		133.8
(3) 		84.5
(-0.8) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 33.6
(-2.9)
A 		125
(0.7)
A 		31.4
(-3)
A 		183.2
(3)
P 		102.6
(-0.2)
A 		89.3
(-0.7)
P 		152
B 101.2
(0.8)
A 		63.3
(-1.2)
A 		28.6
(-3)
A 		142.3
(3)
P 		76.4
(-0.5)
A 		88.6
(0.2)
P 		114
C 111.8
(-2.7)
M 		118.6
(-2.3)
M 		107.5
(-3)
A 		198.7
(3)
P 		129.9
(-1.5)
A 		116
(-2.4)
P 		91
avr 82.2
(-1.7) 		102.3
(-0.7) 		55.8
(-3) 		174.7
(3) 		103
(-0.6) 		98
(-0.9) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 44.2
(3)
A 		10.6
(-2.2)
A 		31.2
(1)
A 		5.1
(-3)
A 		39
B 9.4
(-3)
A 		11.9
(-2.4)
A 		36.5
(3)
A 		20.3
(-0.6)
A 		27
C 28.2
(-1)
A 		21
(-2.9)
A 		43.4
(3)
A 		20.8
(-3)
A 		23
avr 27.3
(0.4) 		14.5
(-3) 		37
(3) 		15.4
(-2.8) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 6.2
(-3)
A 		44.5
(2.7)
A 		6.8
(-2.9)
A 		46.5
(3)
P 		31
(0.7)
A 		21.7
(-0.7)
P 		40
B 35.2
(2.5)
A 		21.1
(-0.2)
A 		6.9
(-3)
A 		37.7
(3)
P 		31
(1.7)
A 		33
(2.1)
P 		31
C 41.5
(2.8)
M 		36.2
(0.6)
M 		27.4
(-3)
A 		40
(2.2)
P 		42
(3)
A 		38.8
(1.7)
P 		15
avr 27.6
(0) 		33.9
(1.4) 		13.7
(-3) 		41.4
(3) 		34.7
(1.5) 		31.2
(0.8) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176237_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18804
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.RR NCBI
Target Description Y105E8B.RR /REP_DB=WormBase Gene ID /WP=CE24101 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026534(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026534 NCBI Caenorhabditis elegans Endoplasmic Reticulum Oxidase family member (ero-1) (ero-1) mRNA, complete cds. 11/11 None
GENEFINDER00000011745 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14672569:14682726:1 11/11 None
Y105E8B.8b ENSEMBL cdna:known chromosome:CEL140:I:14672567:14682726:1 gene:Y105E8B.8 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026533 7/11 Cross Hyb Matching Probes None
SNAP00000011737 8/11 Cross Hyb Matching Probes None
SNAP00000011739 2/11 Cross Hyb Matching Probes None
Y105E8B.8a 7/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173325 2 1 NM_001026534 176237_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14672499-14679010(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y105E8B.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD92388 Hypothetical protein Y105E8B.8b [Caenorhabditis elegans] ref|NP_001021705.1| Endoplasmic Reticulum Oxidase family member (ero-1) [Caenorhabditis elegans] 0.0
blast CAC70110 Hypothetical protein Y105E8B.8a [Caenorhabditis elegans] ref|NP_001021704.1| Endoplasmic Reticulum Oxidase family member (ero-1) [Caenorhabditis elegans] sp|Q7YTU4|ERO1_CAEEL Endoplasmic oxidoreductin 1 precursor 0.0
blast CAE72611 Hypothetical protein CBG19803 [Caenorhabditis briggsae] 1.0E-131
blast CAE72611 Hypothetical protein CBG19803 [Caenorhabditis briggsae] 1.0E-130
Protein Domains
Database ID Description E-Value
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 5.6E-113
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 1.7E-17
Pfam IPR007266 EMBL-EBI Endoplasmic reticulum oxidoreductin 1 5.6E-113
Sequence
Target Sequence 
>C. ELEGANS:176237_AT
gcccattctgggcagacgatcgacaatgtggcacaaatcaatgcggaatcgcattctgtg
atgatgaggttcccgcgggacttcgacgacgaaatgcagtgaatatggtacgcagaagct
ctcatgagagtagtcatgaaatatcagaaaatcataacaacaaccgccaacaacgctgga
atgagacaacgaaaattggggtaattgttcaggaggctgctgctgtgaaagaggaagaag
atgatgatgctgaaaagtgtgcagatgctggaaataatattgatccaatggatagaacac
ttcatgatgatgagaaacgacaattggatgcgatggatcatcatgatgatggactggagg
acaaattctgtgaaattgaagatgacgagtcagatggtatgcactatgtggatttatcga
agaatccggaacgctacacgggatacgctggaaaatcgccgcaaagagtgtggaagagca
tttacgaggagaattgtttcaaaccggatcccaaattcgacaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCCATTCTGGGCAGACGATCGACA 284 281 248 Antisense
ATGCGGAATCGCATTCTGTGATGAT 640 41 288 Antisense
GATGATGAGGTTCCCGCGGGACTTC 136 411 307 Antisense
CCGCGGGACTTCGACGACGAAATGC 344 257 320 Antisense
TATGGTACGCAGAAGCTCTCATGAG 239 655 351 Antisense
GAAAATCATAACAACAACCGCCAAC 492 353 394 Antisense
ACAACCGCCAACAACGCTGGAATGA 479 113 407 Antisense
AATTGTTCAGGAGGCTGCTGCTGTG 440 179 450 Antisense
TCCGGAACGCTACACGGGATACGCT 573 555 672 Antisense
GGGATACGCTGGAAAATCGCCGCAA 522 495 687 Antisense
CAAACCGGATCCCAAATTCGACAAG 371 191 747 Antisense
Sequence Source Affymetrix Proprietary Database