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[176233_at]

Description K08D12.5 /REP_DB=WormBase Gene ID /WP=CE21043 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 50      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 45.4
(-0.9)
A 		87.7
(3)
A 		78.5
(2.2)
A 		21.8
(-3)
A 		66
B 58.1
(-1.7)
A 		74.8
(2.3)
A 		77.8
(3)
A 		52.8
(-3)
A 		25
C 69.6
(-3)
A 		76.6
(3)
A 		70.5
(-2.2)
A 		71.5
(-1.4)
A 		7
avr 57.7
(-1.3) 		79.7
(3) 		75.6
(2.2) 		48.7
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 79.3
(1.9)
A 		88.8
(3)
A 		56
(-0.9)
A 		38.5
(-3)
A 		50.9
(-1.5)
A 		46.6
(-2)
A 		50
B 55.6
(0.5)
A 		87.4
(3)
A 		49.9
(0.1)
A 		31.2
(-1.4)
A 		76.9
(2.2)
A 		10
(-3)
A 		77
C 72
(3)
A 		66.3
(2.1)
A 		41.8
(-1.8)
A 		34.4
(-3)
A 		35.7
(-2.8)
A 		43.7
(-1.5)
A 		38
avr 69
(1.5) 		80.8
(3) 		49.2
(-1) 		34.7
(-2.8) 		54.5
(-0.3) 		33.4
(-3) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 16.5
(1.6)
A 		19
(2.5)
A 		20.5
(3)
A 		3.2
(-3)
A 		17
B 18.5
(3)
A 		18.5
(3)
A 		18
(2.6)
A 		11.2
(-3)
A 		7
C 23
(2.2)
A 		22
(1.8)
A 		24.6
(3)
A 		12.1
(-3)
A 		13
avr 19.3
(2.2) 		19.8
(2.4) 		21
(3) 		8.8
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 14.6
(-1.7)
A 		31.6
(3)
A 		12.2
(-2.3)
A 		9.8
(-3)
A 		15.4
(-1.5)
A 		11.3
(-2.6)
A 		22
B 19.3
(0.4)
A 		29.1
(2.5)
A 		12.1
(-1.2)
A 		8.3
(-2)
A 		31.2
(3)
A 		3.7
(-3)
A 		28
C 26.7
(3)
A 		20.2
(1)
A 		10.6
(-1.9)
A 		6.9
(-3)
A 		11.5
(-1.6)
A 		14.6
(-0.7)
A 		20
avr 20.2
(0.8) 		27
(3) 		11.6
(-1.9) 		8.3
(-3) 		19.4
(0.6) 		9.9
(-2.5) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176233_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10925
Sequence Type Exemplar sequence
Representative Public ID K08D12.5 NCBI
Target Description K08D12.5 /REP_DB=WormBase Gene ID /WP=CE21043 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067722(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067722 NCBI Caenorhabditis elegans K08D12.5 (K08D12.5) mRNA, complete cds. 11/11 None
SNAP00000039168 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1697596:1703272:1 9/11 None
GENEFINDER00000039181 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1699897:1702741:1 9/11 None
K08D12.5 ENSEMBL cdna:known chromosome:CEL140:IV:1699897:1702818:1 gene:K08D12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1699902-1702747(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08D12.5
Entrez Gene 176977 Entrez gene
SwissProt Q966J0 EMBL-EBI
WormBase CE21043 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6486 protein amino acid glycosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8378 galactosyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK84544 Hypothetical protein K08D12.5 [Caenorhabditis elegans] ref|NP_500123.1| K08D12.5 [Caenorhabditis elegans] 1.0E-177
blast AAK84544 Hypothetical protein K08D12.5 [Caenorhabditis elegans] ref|NP_500123.1| K08D12.5 [Caenorhabditis elegans] 1.0E-163
blast CAE67978 Hypothetical protein CBG13584 [Caenorhabditis briggsae] 1.0E-137
blast CAE67978 Hypothetical protein CBG13584 [Caenorhabditis briggsae] 1.0E-136
blast AAK84544 Hypothetical protein K08D12.5 [Caenorhabditis elegans] ref|NP_500123.1| K08D12.5 [Caenorhabditis elegans] 1.0E-132
blast CAE67978 Hypothetical protein CBG13584 [Caenorhabditis briggsae] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR002659 EMBL-EBI Glycosyl transferase, family 31 1.4E-7
Pfam IPR002659 EMBL-EBI Glycosyl transferase, family 31 1.4E-7
Pfam IPR002659 EMBL-EBI Glycosyl transferase, family 31 1.4E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500123.1 1 7-24
K08D12.5 1 7-24
GENEFINDER00000039181 1 7-24
Sequence
Target Sequence 
>C. ELEGANS:176233_AT
ataactttgccaatgcggccgacattccacggggcaacttttcaatagttaaccagtact
acccggacaccaaatgccttgttaaagctgacgtggacaatgttctaattcttcgaaatt
atgagcgactttgcgaagaagcagttgcccctctaattctgggaaaatgcgatgtatcta
gaacggtactacggaataccacaaaatgggccgtgccagaatttgtgtattccgagccag
tttacccgacttattgctccacgggaacttatgtgttggctgggaaaactgtgccgcaga
gtcttatcaaggaagctatgagtagcccatttgcaaattccttgaacttcagaaaactct
cggaagatgtgatattcacgggaattctcgccgaaaaagccggaataaaacgccgtcata
tcaatgggctctcgtttttcgagattcccgaattcttttgtcgaaatggcttcaaaacga
cgtacagtacccatttgttgagtgataagaatcctgtcaagaatttctataaactcatga
aaatcgagggagttctatgcaggtggtggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAACTTTGCCAATGCGGCCGACAT 603 23 329 Antisense
GACATTCCACGGGGCAACTTTTCAA 358 371 349 Antisense
CAGTTGCCCCTCTAATTCTGGGAAA 111 207 470 Antisense
TGGGCCGTGCCAGAATTTGTGTATT 309 555 535 Antisense
AGCCAGTTTACCCGACTTATTGCTC 423 77 563 Antisense
GAAAACTGTGCCGCAGAGTCTTATC 320 355 612 Antisense
GAGTAGCCCATTTGCAAATTCCTTG 443 397 648 Antisense
GATATTCACGGGAATTCTCGCCGAA 189 427 699 Antisense
AACGCCGTCATATCAATGGGCTCTC 583 145 737 Antisense
GGGCTCTCGTTTTTCGAGATTCCCG 532 491 754 Antisense
GAGGGAGTTCTATGCAGGTGGTGGG 64 403 874 Antisense
Sequence Source Affymetrix Proprietary Database