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[176231_at]

Description Y75B7AL.D /REP_DB=WormBase Gene ID /WP=CE26304 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 02      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 70      6grp_total > 24      4grp_total > 38      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 274
1 2 3 4 range
A 356.8
(3)
P 		273.4
(-0.2)
P 		199.4
(-3)
P 		296.6
(0.7)
P 		157
B 423.2
(3)
P 		240.7
(-1)
P 		149.7
(-3)
P 		192.7
(-2.1)
P 		274
C 418.8
(3)
P 		186.1
(-2.7)
P 		172.1
(-3)
P 		247.5
(-1.2)
P 		247
avr 399.6
(3) 		233.4
(-1.4) 		173.7
(-3) 		245.6
(-1.1) 		226

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 472
1 2 3 4 5 6 range
A 430.2
(3)
P 		366.2
(1.7)
P 		294.3
(0.3)
P 		229.9
(-1)
P 		128.4
(-3)
P 		142.8
(-2.7)
P 		302
B 565.5
(3)
P 		374.3
(0.6)
P 		379.5
(0.6)
P 		215
(-1.5)
P 		168.1
(-2.1)
P 		93.6
(-3)
P 		472
C 480.1
(3)
P 		295.4
(-0.1)
P 		305.6
(0)
P 		242.2
(-1)
P 		176.2
(-2.1)
P 		125.7
(-3)
P 		354
avr 491.9
(3) 		345.3
(0.6) 		326.5
(0.3) 		229
(-1.2) 		157.6
(-2.4) 		120.7
(-3) 		371
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 129.9
(3)
P 		59.4
(-1.9)
P 		52.1
(-2.4)
P 		43.4
(-3)
P 		87
B 135
(3)
P 		59.4
(-1.5)
P 		34.7
(-3)
P 		41.1
(-2.6)
P 		100
C 138.6
(3)
P 		53.5
(-2.3)
P 		60
(-1.9)
P 		41.7
(-3)
P 		97
avr 134.5
(3) 		57.4
(-2) 		48.9
(-2.6) 		42.1
(-3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 79.3
(-0.2)
P 		130.3
(3)
P 		64.1
(-1.2)
P 		58.3
(-1.5)
P 		38.7
(-2.8)
P 		34.8
(-3)
P 		96
B 196.8
(3)
P 		124.6
(0.3)
P 		92.2
(-0.9)
P 		56.9
(-2.2)
P 		68.3
(-1.8)
P 		34.9
(-3)
P 		162
C 178
(3)
P 		90.1
(-0.9)
P 		77.8
(-1.4)
P 		48.7
(-2.7)
P 		57
(-2.3)
P 		42.1
(-3)
P 		136
avr 151.4
(3) 		115
(1.1) 		78
(-0.9) 		54.6
(-2.1) 		54.7
(-2.1) 		37.3
(-3) 		114
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176231_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19482
Sequence Type Exemplar sequence
Representative Public ID Y75B7AL.D NCBI
Target Description Y75B7AL.D /REP_DB=WormBase Gene ID /WP=CE26304 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071004(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071004 NCBI Caenorhabditis elegans Y75B7AL.1 (Y75B7AL.1) mRNA, complete cds. 11/11 A 
SNAP00000018276 ENSEMBL cdna:SNAP chromosome:CEL140:V:1351986:1356197:1 11/11 None
GENEFINDER00000018284 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1351974:1356197:1 11/11 None
Y75B7AL.1 ENSEMBL cdna:known chromosome:CEL140:V:1351955:1356406:1 gene:Y75B7AL.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1351985-1356197(+) 95.74 100.0
Public Domain and Genome References
Gene Title
Ensembl Y75B7AL.1
Entrez Gene 178627 Entrez gene
SwissProt Q965S6 EMBL-EBI
WormBase CE31132 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68603 Hypothetical protein Y75B7AL.1 [Caenorhabditis elegans] ref|NP_503405.2| Y75B7AL.1 [Caenorhabditis elegans] 0.0
blast CAE62348 Hypothetical protein CBG06424 [Caenorhabditis briggsae] 0.0
blast AAB88366 Hypothetical protein M04G7.3a [Caenorhabditis elegans] ref|NP_001023318.1| M04G7.3a [Caenorhabditis elegans] 1.0E-22
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503405.2 7 39-61,74-96,118-140,186-203,240-262,292-314,324-346
Y75B7AL.1 7 39-61,74-96,118-140,186-203,240-262,292-314,324-346
SNAP00000018276 7 39-61,74-96,118-140,186-203,240-262,292-314,324-346
GENEFINDER00000018284 7 43-65,78-100,122-144,190-207,244-266,296-318,328-350
Sequence
Target Sequence 
>C. ELEGANS:176231_AT
cgacgaattgtctgatctaccttaaagcgaccgaatggctgaattcccggtttgtagaac
ggaaaactatcaagcgaaagaagactatatgtgattctggacaacttctcggagacaggt
tgtccattttgaagtcatcttgggaaaaagcgaacgagatgacaaatggagaaattggag
tgagagtcgcgtggaacatggttcggaaacatccgaatttgtgtaaaaacgatgaacccg
aaaaagtctcccttctaaacggttcatgtaaacgtagtatcgatcatgccaagtttcaag
agatcggaggtcgaatcacctccttcatatcagagctgcttgagctcatgcaaaataatc
aaccagaaagctatattgttatgagaatccgtcgtgtcggtgcggtgcattatgataagg
gaattgtgttcacatcgtccgtttggaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACGAATTGTCTGATCTACCTTAA 274 251 1112 Antisense
AATGGCTGAATTCCCGGTTTGTAGA 133 159 1145 Antisense
GATTCTGGACAACTTCTCGGAGACA 188 433 1204 Antisense
AGGTTGTCCATTTTGAAGTCATCTT 521 57 1228 Antisense
GAGAGTCGCGTGGAACATGGTTCGG 565 395 1293 Antisense
AGTCTCCCTTCTAAACGGTTCATGT 694 59 1356 Antisense
GTAGTATCGATCATGCCAAGTTTCA 427 455 1385 Antisense
GATCGGAGGTCGAATCACCTCCTTC 61 417 1413 Antisense
TCACCTCCTTCATATCAGAGCTGCT 18 627 1427 Antisense
ATGAGAATCCGTCGTGTCGGTGCGG 315 43 1492 Antisense
TGTGTTCACATCGTCCGTTTGGAAA 697 555 1536 Antisense
Sequence Source Affymetrix Proprietary Database