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[176229_at]

Description F42H10.4 /REP_DB=WormBase Gene ID /WP=CE00166 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 62      6grp_total > 81      4grp_total > 51      6grp_highest_sub > 04_05      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 141.6
(-2.8)
P 		285
(3)
P 		154.8
(-2.2)
P 		135.4
(-3)
P 		150
B 171.5
(-0.5)
P 		232.4
(2.2)
P 		251.2
(3)
P 		115.8
(-3)
P 		135
C 195.6
(1.8)
P 		203.6
(3)
P 		193.1
(1.5)
P 		162.4
(-3)
P 		41
avr 169.6
(-1.1) 		240.3
(3) 		199.7
(0.6) 		137.9
(-3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 923
1 2 3 4 5 6 range
A 245.5
(-2)
P 		404.1
(-0)
P 		421
(0.2)
P 		645
(3)
P 		555.6
(1.9)
P 		164.3
(-3)
P 		481
B 302.1
(-1.3)
P 		341.9
(-0.7)
P 		341.6
(-0.7)
P 		612.2
(3)
P 		592.3
(2.7)
P 		176.7
(-3)
P 		436
C 248.5
(-2.6)
P 		339.7
(-2)
P 		325
(-2.1)
P 		1086.9
(3)
P 		619.4
(-0.1)
P 		184.7
(-3)
P 		902
avr 265.4
(-2.1) 		361.9
(-1.2) 		362.5
(-1.1) 		781.4
(3) 		589.1
(1.1) 		175.2
(-3) 		606
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 51.6
(1.5)
P 		61.9
(3)
P 		40.5
(-0)
P 		19.8
(-3)
P 		42
B 54.7
(2.4)
P 		57.4
(2.9)
P 		58.2
(3)
P 		24.7
(-3)
P 		34
C 64.8
(2.6)
P 		58.5
(1.7)
P 		67.3
(3)
P 		27.4
(-3)
P 		40
avr 57
(2.6) 		59.3
(3) 		55.3
(2.3) 		24
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 45.3
(-2.8)
P 		143.7
(1.9)
P 		91.7
(-0.6)
P 		163.6
(2.8)
P 		167.7
(3)
P 		40
(-3)
P 		128
B 105.1
(-1.7)
P 		113.8
(-1.4)
P 		82.9
(-2.4)
P 		162.1
(0.3)
P 		240.6
(3)
P 		65.9
(-3)
P 		175
C 92.1
(-1.8)
P 		103.7
(-1.4)
P 		82.7
(-2.2)
P 		218.6
(3)
P 		200.4
(2.3)
P 		61.8
(-3)
P 		157
avr 80.8
(-2) 		120.4
(-0.4) 		85.8
(-1.8) 		181.4
(2.1) 		202.9
(3) 		55.9
(-3) 		147
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176229_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6889
Sequence Type Exemplar sequence
Representative Public ID F42H10.4 NCBI
Target Description F42H10.4 /REP_DB=WormBase Gene ID /WP=CE00166 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066473
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066473 NCBI Caenorhabditis elegans F42H10.3 (F42H10.3) mRNA, complete cds. None
F42H10.3 ENSEMBL cdna:known chromosome:CEL140:III:8484066:8488030:1 gene:F42H10.3 A 
SNAP00000027853 ENSEMBL cdna:SNAP chromosome:CEL140:III:8484066:8485437:1 None
GENEFINDER00000027861 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8484066:8486369:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8484060-8485347(+) 98.5 100.0
Public Domain and Genome References
Gene Title
Ensembl F42H10.3
Entrez Gene 185685 Entrez gene
SwissProt P34416 EMBL-EBI
WormBase CE37108 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSGENOME1:148909_AT dm
DROSOPHILA_2:1637874_AT dm
Protein Similarities
Method ID Description E-Value
blast AAT92076 Hypothetical protein F42H10.3 [Caenorhabditis elegans] ref|NP_498874.2| F42H10.3 [Caenorhabditis elegans] sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3 1.0E-163
blast CAE71248 Hypothetical protein CBG18124 [Caenorhabditis briggsae] 7.0E-90
blast CAE71248 Hypothetical protein CBG18124 [Caenorhabditis briggsae] 3.0E-88
blast AAT92076 Hypothetical protein F42H10.3 [Caenorhabditis elegans] ref|NP_498874.2| F42H10.3 [Caenorhabditis elegans] sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3 2.0E-85
blast AAT92076 Hypothetical protein F42H10.3 [Caenorhabditis elegans] ref|NP_498874.2| F42H10.3 [Caenorhabditis elegans] sp|P34416|LASP1_CAEEL LIM and SH3 domain protein F42H10.3 4.0E-85
blast CAE71248 Hypothetical protein CBG18124 [Caenorhabditis briggsae] 4.0E-75
blast CAE71247 Hypothetical protein CBG18123 [Caenorhabditis briggsae] 3.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR000900 EMBL-EBI Nebulin 5.2E-11
Pfam IPR000900 EMBL-EBI Nebulin 1.0E-4
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 3.4E-13
Pfam IPR001452 EMBL-EBI SH3 3.3E-22
Sequence
Target Sequence 
>C. ELEGANS:176229_AT
aatgcttcaaatgcactgtttgcggaatgacattgaatatgaaaaactataagggatacg
acaagagaccgtattgtgatccacattatccaaaaaccgtggcttcagtgatggcggata
ctccagaaatgagaagaatcgcagagaatactaaaaatcagagtaacatcaaataccacg
ccgaatacgagaaaatgaagggaacaaagatcgaaattgctgatgatccagagatggaaa
gactgaagaaaaatactcaggttcaatcaaatgtttcctatcacggagttctcgaccaaa
aagctcgtcaagaagaagtccgtccaaaagaggaaatctcaccaaacccaactccaagta
agttgttcttccattttgttgtccccatggacttgagaattgtatcgtccaagttatgtc
aaaagtcttgcacgggaaaaacttttaattcttttttcaattttcctcacttttctctta
ttgtttttgtatcgctttttactgccagtttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCTTCAAATGCACTGTTTGCGG 18 167 101 Antisense
AAGAGACCGTATTGTGATCCACATT 289 153 163 Antisense
GTGGCTTCAGTGATGGCGGATACTC 617 491 199 Antisense
AACATCAAATACCACGCCGAATACG 535 133 265 Antisense
ATGTTTCCTATCACGGAGTTCTCGA 162 47 371 Antisense
GAGTTCTCGACCAAAAAGCTCGTCA 547 395 386 Antisense
AGCTCGTCAAGAAGAAGTCCGTCCA 95 81 402 Antisense
AGAGGAAATCTCACCAAACCCAACT 399 65 429 Antisense
TCTTCCATTTTGTTGTCCCCATGGA 146 617 467 Antisense
TCCTCACTTTTCTCTTATTGTTTTT 555 591 564 Antisense
GTATCGCTTTTTACTGCCAGTTTAT 335 455 589 Antisense
Sequence Source Affymetrix Proprietary Database