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[176227_s_at]

Description Y71H2AM.I /REP_DB=WormBase Gene ID /WP=CE22951 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 15      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 60      6grp_total > 25      4grp_total > 51      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 617.1
(0.9)
P 		787.3
(3)
P 		639.4
(1.2)
P 		294.6
(-3)
P 		493
B 549.1
(0.4)
P 		722.5
(3)
P 		619.4
(1.5)
P 		322.8
(-3)
P 		400
C 558.5
(-0.1)
P 		697.6
(3)
P 		558.6
(-0.1)
P 		426.2
(-3)
P 		271
avr 574.9
(0.5) 		735.8
(3) 		605.8
(1) 		347.9
(-3) 		388

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 473
1 2 3 4 5 6 range
A 526.4
(1.4)
P 		635.6
(3)
P 		241.2
(-2.8)
P 		268.5
(-2.4)
P 		225.6
(-3)
P 		230.7
(-2.9)
P 		410
B 564.5
(3)
P 		561.7
(3)
P 		368.3
(0.1)
P 		275.2
(-1.3)
P 		180
(-2.7)
P 		162.8
(-3)
P 		402
C 608
(3)
P 		568.7
(2.5)
P 		421.1
(0.5)
P 		168.2
(-3)
P 		227.5
(-2.2)
P 		170.5
(-3)
P 		440
avr 566.3
(2.7) 		588.7
(3) 		343.5
(-0.7) 		237.3
(-2.3) 		211
(-2.7) 		188
(-3) 		401
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 224.7
(3)
P 		170.9
(1.2)
P 		167.1
(1.1)
P 		43.1
(-3)
P 		182
B 175.1
(2.8)
P 		178.5
(3)
P 		143.5
(1.1)
P 		68.8
(-3)
P 		110
C 184.9
(2.3)
P 		200.6
(3)
P 		194.7
(2.7)
P 		71.9
(-3)
P 		129
avr 194.9
(3) 		183.3
(2.5) 		168.4
(1.8) 		61.3
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 192
1 2 3 4 5 6 range
A 97
(-1.5)
P 		226
(3)
P 		52.5
(-3)
P 		68.1
(-2.5)
P 		68.1
(-2.5)
P 		56.2
(-2.9)
P 		174
B 196.4
(3)
P 		187
(2.6)
P 		89.4
(-1.7)
P 		72.9
(-2.5)
P 		73.1
(-2.5)
P 		60.7
(-3)
P 		136
C 225.4
(3)
P 		173.5
(1.4)
P 		107.2
(-0.7)
P 		33.8
(-3)
P 		73.6
(-1.8)
P 		57.1
(-2.3)
P 		192
avr 172.9
(2) 		195.5
(3) 		83
(-1.9) 		58.3
(-3) 		71.6
(-2.4) 		58
(-3) 		138
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176227_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18952
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.I NCBI
Target Description Y71H2AM.I /REP_DB=WormBase Gene ID /WP=CE22951 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065210(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065210 NCBI Caenorhabditis elegans Y71H2AM.15 (Y71H2AM.15) mRNA, complete cds. 11/11 A 
SNAP00000014443 ENSEMBL cdna:SNAP chromosome:CEL140:III:2747227:2756350:-1 11/11 None
GENEFINDER00000014459 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2747227:2756350:-1 11/11 None
Y71H2AM.15 ENSEMBL cdna:known chromosome:CEL140:III:2747120:2756350:-1 gene:Y71H2AM.15 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2747226-2752669(-) 97.28 100.0
Public Domain and Genome References
Ensembl Y71H2AM.15
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29981 Hypothetical protein Y71H2AM.15a [Caenorhabditis elegans] ref|NP_497611.1| Y71H2AM.15 [Caenorhabditis elegans] 0.0
blast CAE64978 Hypothetical protein CBG09813 [Caenorhabditis briggsae] 0.0
blast ABA00158 Hypothetical protein Y71H2AM.15b [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:176227_S_AT
ggtgcaagatccatcgattcaacgtaggaagaagcatgtggaacgggcggattcagctgg
agctccaggatctccaagattacctccaaaaccttctgtagcacagacaattgctatgca
ctcagctccaccatcaccgttgacagctccgatcccacctccaccaccgccaccaccgcc
gatgcttccaccacaacaatctcagctcgcccagtccctccaactagcggctaccagttc
taatgtggtaaaggttgctccaatgtatgagtacaaggaatccccaaagtcgcctgtaag
tgatcttgacaatgttctcgaagaagttggagatccgggcagcatcccacgtcctccaag
ccgatactctgtccaagacgttcgacaaaagttctccaaaccgccagagctgccagatca
gct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTGCAAGATCCATCGATTCAACGT 77 501 2001 Antisense
TGTGGAACGGGCGGATTCAGCTGGA 122 555 2037 Antisense
GAGCTCCAGGATCTCCAAGATTACC 652 385 2060 Antisense
GATTACCTCCAAAACCTTCTGTAGC 116 431 2078 Antisense
GACAATTGCTATGCACTCAGCTCCA 27 365 2106 Antisense
ACTAGCGGCTACCAGTTCTAATGTG 373 101 2223 Antisense
ACAAGGAATCCCCAAAGTCGCCTGT 411 107 2273 Antisense
GAAGTTGGAGATCCGGGCAGCATCC 586 337 2323 Antisense
GCCGATACTCTGTCCAAGACGTTCG 310 285 2360 Antisense
CGACAAAAGTTCTCCAAACCGCCAG 533 71 2383 Antisense
AACCGCCAGAGCTGCCAGATCAGCT 544 145 2399 Antisense
Sequence Source Affymetrix Proprietary Database