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[176225_at]

Description Y71H2AM.T /REP_DB=WormBase Gene ID /WP=CE22940 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 37      6grp_total > 42      4grp_total > 59      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3232
1 2 3 4 range
A 4811
(-0.1)
P 		5514.6
(1.7)
P 		5995.8
(3)
P 		3738
(-3)
P 		2258
B 4816.2
(-0.2)
P 		4848.6
(-0.1)
P 		6544.2
(3)
P 		3312.6
(-3)
P 		3232
C 4388
(0.1)
P 		5351.8
(3)
P 		4259.7
(-0.3)
P 		3385.5
(-3)
P 		1966
avr 4671.7
(0.4) 		5238.3
(2) 		5599.9
(3) 		3478.7
(-3) 		2121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2684
1 2 3 4 5 6 range
A 5464.9
(0.8)
P 		5058
(-0.1)
P 		6393.8
(3)
P 		6139.9
(2.4)
P 		3835
(-3)
P 		4142.3
(-2.3)
P 		2559
B 4484.2
(0.1)
P 		4748.8
(1.2)
P 		5188.2
(3)
P 		4684.6
(1)
P 		4347.7
(-0.4)
P 		3710.3
(-3)
P 		1478
C 4943.7
(0.1)
P 		4273.1
(-1.7)
P 		6006.2
(3)
P 		4290.5
(-1.7)
P 		4931.8
(0.1)
P 		3805.3
(-3)
P 		2201
avr 4964.3
(0.3) 		4693.3
(-0.5) 		5862.7
(3) 		5038.3
(0.5) 		4371.5
(-1.5) 		3886
(-3) 		1977
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1205
1 2 3 4 range
A 1751.7
(3)
P 		1197.1
(0.2)
P 		1567.4
(2.1)
P 		547.2
(-3)
P 		1205
B 1535.8
(3)
P 		1197.5
(0.6)
P 		1516.4
(2.9)
P 		705.9
(-3)
P 		830
C 1452.5
(2.5)
P 		1538.7
(3)
P 		1484.6
(2.7)
P 		570.8
(-3)
P 		968
avr 1580
(3) 		1311.1
(1.3) 		1522.8
(2.6) 		608
(-3) 		972

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 970
1 2 3 4 5 6 range
A 1007.4
(-3)
P 		1798.9
(3)
P 		1392.4
(-0.1)
P 		1557.2
(1.2)
P 		1157.3
(-1.9)
P 		1008.5
(-3)
P 		792
B 1560.3
(0.6)
P 		1580.7
(0.9)
P 		1259.8
(-2.8)
P 		1240.8
(-3)
P 		1766.4
(3)
P 		1383.5
(-1.4)
P 		526
C 1832.4
(3)
P 		1303.9
(-0.3)
P 		1528.7
(1.1)
P 		862.8
(-3)
P 		1595.9
(1.5)
P 		1273.8
(-0.5)
P 		970
avr 1466.7
(1.3) 		1561.2
(3) 		1393.6
(0.1) 		1220.3
(-3) 		1506.5
(2) 		1221.9
(-3) 		341
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18953
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.T NCBI
Target Description Y71H2AM.T /REP_DB=WormBase Gene ID /WP=CE22940 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065218(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065218 NCBI Caenorhabditis elegans Y71H2AM.4 (Y71H2AM.4) mRNA, complete cds. 11/11 None
SNAP00000014450 ENSEMBL cdna:SNAP chromosome:CEL140:III:2786852:2787953:1 11/11 None
GENEFINDER00000014472 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2786852:2787953:1 11/11 None
Y71H2AM.4.2 ENSEMBL cdna:known chromosome:CEL140:III:2786842:2788018:1 gene:Y71H2AM.4 11/11 None
Y71H2AM.4.1 ENSEMBL cdna:known chromosome:CEL140:III:2786844:2788018:1 gene:Y71H2AM.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2786851-2787953(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.4
Entrez Gene 175397 Entrez gene
SwissProt Q9BL33 EMBL-EBI
WormBase CE22940 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29975 Hypothetical protein Y71H2AM.4 [Caenorhabditis elegans] ref|NP_497619.1| Y71H2AM.4 [Caenorhabditis elegans] 1.0E-67
blast CAE64985 Hypothetical protein CBG09820 [Caenorhabditis briggsae] 4.0E-60
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497619.1 2 32-54,64-86
Y71H2AM.4.2 2 32-54,64-86
Y71H2AM.4.1 2 32-54,64-86
SNAP00000014450 2 32-54,64-86
GENEFINDER00000014472 2 32-54,64-86
Sequence
Target Sequence 
>C. ELEGANS:176225_AT
atggctgactcaactcgcgtcagcgagaaggaaatcgagcgccgcgagacctttattcgc
gcaggacacagagctcgtcacctggccgatccattcacatggagctacccattgaagggt
gccagtgcaatgctcggaatctcgctggcttcggtttatttgcaaaatcgatggaacaag
aagccgtactactacgcaattgttccacggcttttgctcatcggagccgccaccgccgcc
ggatacgctgccggagcacttcgagagagacactatcagacgagagacgcagtcatcgag
cactacatgaagcttcatccagaggatttcgaccattttaatgatcgcagtggacgttca
ttctctcaagttcttcttccatggta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCTGACTCAACTCGCGTCAGCG 592 53 13 Antisense
AGCGCCGCGAGACCTTTATTCGCGC 249 83 50 Antisense
TATTCGCGCAGGACACAGAGCTCGT 282 661 66 Antisense
CCTGGCCGATCCATTCACATGGAGC 103 267 93 Antisense
TGAAGGGTGCCAGTGCAATGCTCGG 481 575 125 Antisense
TCTCGCTGGCTTCGGTTTATTTGCA 349 611 152 Antisense
ACTACTACGCAATTGTTCCACGGCT 227 101 200 Antisense
CGCTGCCGGAGCACTTCGAGAGAGA 430 255 258 Antisense
GAGAGACGCAGTCATCGAGCACTAC 382 395 294 Antisense
TGATCGCAGTGGACGTTCATTCTCT 443 565 354 Antisense
TCTCTCAAGTTCTTCTTCCATGGTA 664 611 374 Antisense
Sequence Source Affymetrix Proprietary Database