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[176224_at]

Description Y69A2AR.A /REP_DB=WormBase Gene ID /WP=CE26557 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 25      4grp_total > 53      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 134.2
(3)
P 		61.1
(-0.4)
P 		59.5
(-0.5)
P 		4.6
(-3)
A 		130
B 91.6
(3)
P 		90.6
(2.9)
M 		45.1
(-0.2)
A 		3.2
(-3)
A 		88
C 84.1
(2.2)
P 		95.7
(3)
P 		63.4
(0.8)
M 		6.1
(-3)
A 		90
avr 103.3
(3) 		82.5
(1.7) 		56
(0.1) 		4.6
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 109.9
(3)
P 		81.4
(1.4)
P 		30
(-1.6)
A 		36
(-1.2)
A 		23.6
(-1.9)
A 		5.1
(-3)
A 		105
B 65
(3)
A 		53.4
(1.1)
A 		28.6
(-3)
A 		48.3
(0.2)
A 		30.2
(-2.7)
A 		34.9
(-2)
P 		36
C 68.1
(0.4)
P 		95.8
(2.5)
P 		102.4
(3)
P 		22.6
(-3)
A 		42
(-1.5)
A 		44.4
(-1.4)
A 		80
avr 81
(3) 		76.9
(2.5) 		53.7
(-0.1) 		35.6
(-2.1) 		31.9
(-2.6) 		28.1
(-3) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 48.9
(3)
P 		13.3
(-1.4)
P 		15.6
(-1.1)
P 		0.7
(-3)
A 		48
B 29.2
(3)
P 		22.4
(1.6)
M 		10.5
(-0.9)
A 		0.7
(-3)
A 		29
C 27.8
(3)
P 		27.5
(2.9)
P 		22.1
(1.7)
M 		1
(-3)
A 		27
avr 35.3
(3) 		21.1
(0.5) 		16.1
(-0.3) 		0.8
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 20.3
(1.1)
P 		29
(3)
P 		6.5
(-1.9)
A 		9.1
(-1.3)
A 		7.1
(-1.7)
A 		1.3
(-3)
A 		28
B 22.6
(3)
A 		17.8
(1.2)
A 		6.9
(-3)
A 		12.8
(-0.7)
A 		12.3
(-0.9)
A 		13
(-0.7)
P 		16
C 25.3
(2)
P 		29.2
(3)
P 		26.1
(2.2)
P 		4.6
(-3)
A 		13.6
(-0.8)
A 		14.9
(-0.5)
A 		25
avr 22.7
(2.1) 		25.3
(3) 		13.2
(-1.4) 		8.8
(-3) 		11
(-2.2) 		9.7
(-2.7) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176224_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19373
Sequence Type Exemplar sequence
Representative Public ID Y69A2AR.A NCBI
Target Description Y69A2AR.A /REP_DB=WormBase Gene ID /WP=CE26557 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067839(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067839 NCBI Caenorhabditis elegans Y69A2AR.31 (Y69A2AR.31) mRNA, complete cds. 10/11 A 
GENEFINDER00000018840 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2650489:2663963:-1 10/11 None
Y69A2AR.31 ENSEMBL cdna:known chromosome:CEL140:IV:2650381:2664569:-1 gene:Y69A2AR.31 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000018829 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2659413-2664575(-) 91.65 100.0
Public Domain and Genome References
Gene Title
Ensembl Y69A2AR.31
Entrez Gene 177049 Entrez gene
SwissProt Q95XG5 EMBL-EBI
WormBase CE27519 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68577 Hypothetical protein Y69A2AR.31 [Caenorhabditis elegans] ref|NP_500240.1| Y69A2AR.31 [Caenorhabditis elegans] 0.0
blast CAE68242 Hypothetical protein CBG13916 [Caenorhabditis briggsae] 0.0
blast XP_793677 PREDICTED: similar to glial cell line derived neurotrophic factor family receptor alpha 2, partial [Strongylocentrotus purpuratus] 1.0E-10
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500240.1 1 12-34
Y69A2AR.31 1 12-34
GENEFINDER00000018840 2 1037-1059,1107-1129
Sequence
Target Sequence 
>C. ELEGANS:176224_AT
aaaaacgtgcgacgcagcgctctaccaagtttgcctaaaacacgtgtcttgctctcaact
ttggtcaatgtttcgcaagaattgtgacgtggatctggataatcagtgtcgaatggctga
tcgagaagtgtgctggcagagttttgaggggctcacgtggagtgggctcggcgattgtca
gtgtgcttcttcgaattcgtcggattgtcattggattcgattgcatactaattataataa
atgtatatacgaaatttcgaaatccggacaatttcctgtcttgatgactttggctcagaa
aaaccgtgaagctgaggagaataagcagcgagcaatgagtgagcagtatcgaagaagata
tgaaaatcgcgaacaatctgtttccctgattttccagaatatattctccgtgaagttcca
actaccacaacaaccacaacaaccactacagtacccccaacaacaacgaggaccacaacc
acaactaccccaaggcctacagtacctacagtaccccggacaactaccgtacccagttgg
tggagctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAACGTGCGACGCAGCGCTCTAC 598 129 621 Antisense
GCGCTCTACCAAGTTTGCCTAAAAC 387 287 637 Antisense
TAAAACACGTGTCTTGCTCTCAACT 549 639 656 Antisense
TTGAGGGGCTCACGTGGAGTGGGCT 552 697 764 Antisense
GATTGTCAGTGTGCTTCTTCGAATT 27 435 793 Antisense
GAATTCGTCGGATTGTCATTGGATT 244 325 813 Antisense
AAATCCGGACAATTTCCTGTCTTGA 33 117 880 Antisense
CCTGTCTTGATGACTTTGGCTCAGA 36 267 895 Antisense
GAACAATCTGTTTCCCTGATTTTCC 465 353 991 Antisense
TATTCTCCGTGAAGTTCCAACTACC 253 659 1022 Antisense
TACCGTACCCAGTTGGTGGAGCTCC 352 647 1145 Antisense
Sequence Source Affymetrix Proprietary Database