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[176223_at]

Description Y82E9BL.B /REP_DB=WormBase Gene ID /WP=CE23418 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 74      4grp_total > 35      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 45.6
(-0.7)
A 		80.7
(1.9)
A 		13.3
(-3)
A 		95.8
(3)
A 		83
B 10.5
(-1.9)
A 		22.7
(-0.1)
A 		43.3
(3)
A 		3.5
(-3)
A 		40
C 8.9
(-2.7)
A 		121.6
(3)
A 		2.6
(-3)
A 		101
(2)
A 		119
avr 21.7
(-2.8) 		75
(3) 		19.7
(-3) 		66.8
(2.1) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 28
(-3)
A 		40.3
(-1.4)
A 		39.7
(-1.5)
A 		74.7
(3)
A 		34.8
(-2.1)
A 		50.8
(-0.1)
A 		47
B 29.6
(-0.2)
A 		19.1
(-1.4)
A 		4.5
(-3)
A 		51.2
(2.2)
A 		58.9
(3)
A 		49.1
(1.9)
A 		54
C 21.9
(-1.5)
A 		59
(3)
A 		51.2
(2.1)
A 		45.7
(1.4)
A 		9.7
(-3)
A 		40.2
(0.7)
A 		49
avr 26.5
(-3) 		39.5
(-0.5) 		31.8
(-2) 		57.2
(3) 		34.5
(-1.4) 		46.7
(0.9) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 16.6
(2.6)
A 		17.5
(3)
A 		3.5
(-3)
A 		14
(1.5)
A 		14
B 3.3
(-1.3)
A 		5.6
(0.2)
A 		10
(3)
A 		0.7
(-3)
A 		9
C 2.9
(-2.6)
A 		35
(3)
A 		0.9
(-3)
A 		17
(-0.2)
A 		34
avr 7.6
(-1.8) 		19.4
(3) 		4.8
(-3) 		10.6
(-0.6) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 5.2
(-3)
A 		14.3
(1)
A 		8.6
(-1.5)
A 		18.9
(3)
A 		10.5
(-0.7)
A 		12.4
(0.2)
A 		14
B 10.3
(-0.6)
A 		6.4
(-1.6)
A 		1.1
(-3)
A 		13.6
(0.3)
A 		23.9
(3)
A 		18.3
(1.5)
A 		23
C 8.1
(-1)
A 		18
(3)
A 		13
(1)
A 		9.2
(-0.5)
A 		3.1
(-3)
A 		13.5
(1.2)
A 		15
avr 7.9
(-2.7) 		12.9
(1.5) 		7.6
(-3) 		13.9
(2.3) 		12.5
(1.1) 		14.7
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176223_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19045
Sequence Type Exemplar sequence
Representative Public ID Y82E9BL.B NCBI
Target Description Y82E9BL.B /REP_DB=WormBase Gene ID /WP=CE23418 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064991(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064991 NCBI Caenorhabditis elegans Y82E9BL.2 (Y82E9BL.2) mRNA, complete cds. 11/11 None
SNAP00000041775 ENSEMBL cdna:SNAP chromosome:CEL140:III:1351206:1353628:-1 11/11 None
Y82E9BL.2 ENSEMBL cdna:known chromosome:CEL140:III:1351206:1353808:-1 gene:Y82E9BL.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1351205-1353038(-) 93.22 93.22
Public Domain and Genome References
Gene Title
Ensembl Y82E9BL.2
Entrez Gene 190751 Entrez gene
SwissProt Q9GR29 EMBL-EBI
WormBase CE23418 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG37969 Hypothetical protein Y82E9BL.2 [Caenorhabditis elegans] ref|NP_497392.1| Y82E9BL.2 [Caenorhabditis elegans] 1.0E-130
blast AAG37969 Hypothetical protein Y82E9BL.2 [Caenorhabditis elegans] ref|NP_497392.1| Y82E9BL.2 [Caenorhabditis elegans] 1.0E-119
blast AAK66032 Hypothetical protein Y82E9BL.5 [Caenorhabditis elegans] ref|NP_497389.1| Y82E9BL.5 [Caenorhabditis elegans] 1.0E-48
blast AAK66032 Hypothetical protein Y82E9BL.5 [Caenorhabditis elegans] ref|NP_497389.1| Y82E9BL.5 [Caenorhabditis elegans] 2.0E-48
blast AAG37970 Hypothetical protein Y82E9BL.3 [Caenorhabditis elegans] ref|NP_497391.1| Y82E9BL.3 [Caenorhabditis elegans] 2.0E-47
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497392.1 2 132-154,169-191
Y82E9BL.2 2 132-154,169-191
SNAP00000041775 2 165-187,202-224
Sequence
Target Sequence 
>C. ELEGANS:176223_AT
gattttatcttgaccatccaaccatcgcaaacttttctaccacttatgcacttcctacac
tctctgctgatggatgccagtacatgcgtagttgcgcccaagctggagagcatttgactt
attacattccattggataaacctgatgataggccgcaccttataagcacccttgcctcct
tatttatttatttcctactgcaatgccttccatatggttttgcaagtattctttcagagt
cagaatcacctatcaagtacacttttccaaaaaccacaaaactaaccaaccttcaaaaag
tatcaatcttcacattcttcaccattatggaagtctactatatctacttgtgcacttgga
ctcatccatctcagaacgagtataacattgtcaaattcttaccaggaactatatacttat
gcccaataatcaatataatggttatacctcccgttgttgtagcgtgctatgcttataact
cggatacagtaaaattatttcaatcgcaacctggtagtgaactacagtttattggactca
aaacatggaatcctgcaactggagtctggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTTATCTTGACCATCCAACCAT 439 429 107 Antisense
CCTACACTCTCTGCTGATGGATGCC 89 269 160 Antisense
GATGGATGCCAGTACATGCGTAGTT 632 407 175 Antisense
TGCGTAGTTGCGCCCAAGCTGGAGA 37 585 191 Antisense
TGATAGGCCGCACCTTATAAGCACC 619 563 252 Antisense
TTCCTACTGCAATGCCTTCCATATG 522 699 298 Antisense
CTTGTGCACTTGGACTCATCCATCT 5 217 453 Antisense
TAATGGTTATACCTCCCGTTGTTGT 505 635 542 Antisense
GCGTGCTATGCTTATAACTCGGATA 628 291 568 Antisense
ATTATTTCAATCGCAACCTGGTAGT 526 1 600 Antisense
GGAATCCTGCAACTGGAGTCTGGGA 228 533 653 Antisense
Sequence Source Affymetrix Proprietary Database