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[176222_at]

Description Y82E9BL.H /REP_DB=WormBase Gene ID /WP=CE23393 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 80      6grp_total > 32      4grp_total > 46      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 428.6
(3)
P 		222
(-2.1)
P 		238.9
(-1.7)
P 		187.9
(-3)
P 		241
B 455.9
(3)
P 		214.8
(-1.8)
P 		219.4
(-1.7)
P 		154.4
(-3)
P 		302
C 433
(3)
P 		285.1
(-1)
P 		208.4
(-3)
P 		215.8
(-2.8)
P 		225
avr 439.2
(3) 		240.6
(-1.7) 		222.2
(-2.1) 		186
(-3) 		253

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 249
1 2 3 4 5 6 range
A 271.8
(1.7)
P 		314.1
(3)
P 		307.7
(2.8)
P 		205.2
(-0.4)
P 		151.2
(-2.1)
P 		120.6
(-3)
P 		194
B 275.2
(1.3)
P 		234.6
(0.4)
P 		344.6
(3)
P 		198.1
(-0.5)
P 		143.4
(-1.8)
P 		95.5
(-3)
P 		249
C 254.9
(1)
P 		273.5
(1.6)
P 		315.4
(3)
P 		215.1
(-0.4)
P 		160.3
(-2.2)
P 		136.6
(-3)
P 		179
avr 267.3
(1.4) 		274.1
(1.6) 		322.6
(3) 		206.1
(-0.4) 		151.6
(-2) 		117.6
(-3) 		205
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 156
(3)
P 		48.2
(-2)
P 		62.4
(-1.4)
P 		27.5
(-3)
P 		129
B 145.4
(3)
P 		53.1
(-1.9)
P 		50.8
(-2)
P 		32.9
(-3)
P 		113
C 143.3
(3)
P 		82
(-0.4)
P 		72.6
(-1)
P 		36.4
(-3)
P 		107
avr 148.2
(3) 		61.1
(-1.5) 		61.9
(-1.5) 		32.3
(-3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 50.1
(-1.5)
P 		111.7
(3)
P 		67
(-0.3)
P 		52.1
(-1.3)
P 		45.6
(-1.8)
P 		29.4
(-3)
P 		82
B 95.7
(3)
P 		78.1
(1.2)
P 		83.7
(1.8)
P 		52.5
(-1.3)
P 		58.2
(-0.7)
P 		35.6
(-3)
P 		60
C 94.5
(3)
P 		83.5
(1.7)
P 		80.3
(1.3)
P 		43.3
(-3)
P 		51.9
(-2)
P 		45.7
(-2.7)
P 		51
avr 80.1
(1.8) 		91.1
(3) 		77
(1.4) 		49.3
(-1.6) 		51.9
(-1.3) 		36.9
(-3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176222_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19054
Sequence Type Exemplar sequence
Representative Public ID Y82E9BL.H NCBI
Target Description Y82E9BL.H /REP_DB=WormBase Gene ID /WP=CE23393 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064983(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064983 NCBI Caenorhabditis elegans Y82E9BL.10 (Y82E9BL.10) mRNA, complete cds. 11/11 None
SNAP00000041768 ENSEMBL cdna:SNAP chromosome:CEL140:III:1322138:1323621:-1 11/11 None
GENEFINDER00000041780 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1322138:1323621:-1 11/11 None
Y82E9BL.10 ENSEMBL cdna:known chromosome:CEL140:III:1322138:1323621:-1 gene:Y82E9BL.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1322137-1323621(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y82E9BL.10
Entrez Gene 175294 Entrez gene
SwissProt Q9GR36 EMBL-EBI
WormBase CE23393 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG37975 Hypothetical protein Y82E9BL.10 [Caenorhabditis elegans] ref|NP_497384.1| Y82E9BL.10 [Caenorhabditis elegans] 1.0E-164
blast AAG37976 Hypothetical protein Y82E9BL.11 [Caenorhabditis elegans] ref|NP_497383.1| Y82E9BL.11 [Caenorhabditis elegans] 1.0E-134
blast AAG37975 Hypothetical protein Y82E9BL.10 [Caenorhabditis elegans] ref|NP_497384.1| Y82E9BL.10 [Caenorhabditis elegans] 1.0E-129
blast AAG37976 Hypothetical protein Y82E9BL.11 [Caenorhabditis elegans] ref|NP_497383.1| Y82E9BL.11 [Caenorhabditis elegans] 1.0E-103
blast AAC04395 Hypothetical protein C17B7.11 [Caenorhabditis elegans] ref|NP_503929.1| C17B7.11 [Caenorhabditis elegans] 4.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 5.3E-53
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 5.3E-53
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 9.5E-13
Sequence
Target Sequence 
>C. ELEGANS:176222_AT
aatgatgctctttctatacttccttattttaatgcggacatgctggatatcatccactta
tcgatttctgacaatattagtcaatttgaaagaataacatatctggatcaatggaaaagc
gcaagatccttccatcttagctatgttaactttgacgatgattacattgagcatttgttc
cgcttcgaagagttttccatttcattgccgtacctattaacagatactgtggttgatatc
atagatgatctcttaacaaggcccacattccgagagtgttgtattgagtttgaaggtgat
gaagttaaagccgatcaagtcctaagactatttgatccggaccggccagcctatagtagt
ga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGATGCTCTTTCTATACTTCCTT 254 165 463 Antisense
ATGCGGACATGCTGGATATCATCCA 497 41 494 Antisense
TATCATCCACTTATCGATTTCTGAC 592 659 510 Antisense
GATCCTTCCATCTTAGCTATGTTAA 99 417 587 Antisense
TACATTGAGCATTTGTTCCGCTTCG 25 645 625 Antisense
TCCGCTTCGAAGAGTTTTCCATTTC 78 555 641 Antisense
CCATTTCATTGCCGTACCTATTAAC 554 269 659 Antisense
GCCCACATTCCGAGAGTGTTGTATT 409 287 723 Antisense
GTTAAAGCCGATCAAGTCCTAAGAC 328 433 766 Antisense
CTAAGACTATTTGATCCGGACCGGC 4 225 784 Antisense
CGGACCGGCCAGCCTATAGTAGTGA 689 241 800 Antisense
Sequence Source Affymetrix Proprietary Database