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[176220_at]

Description Y71H2AM.C /REP_DB=WormBase Gene ID /WP=CE22941 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 02      4grp_total > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 34.6
(-0.7)
A 		7.5
(-3)
A 		38.9
(-0.4)
A 		79.3
(3)
P 		72
B 17.8
(-3)
A 		24.6
(-1.8)
A 		50.6
(3)
M 		35.9
(0.3)
A 		33
C 57.1
(3)
A 		21.6
(-3)
A 		36.8
(-0.4)
A 		28
(-1.9)
A 		36
avr 36.5
(0.7) 		17.9
(-3) 		42.1
(1.9) 		47.7
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 58.8
(0.7)
P 		65.4
(1.3)
P 		10.7
(-3)
A 		20.3
(-2.3)
A 		87.8
(3)
P 		37.2
(-0.9)
A 		77
B 61.4
(3)
P 		58.3
(2.3)
P 		36.4
(-3)
A 		50.2
(0.3)
P 		57
(1.9)
P 		38.9
(-2.4)
A 		25
C 46.9
(0.1)
P 		64
(3)
P 		32.2
(-2.5)
A 		54.3
(1.3)
A 		34.3
(-2.1)
A 		29
(-3)
A 		35
avr 55.7
(1.9) 		62.6
(3) 		26.4
(-3) 		41.6
(-0.5) 		59.7
(2.5) 		35
(-1.6) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 12.6
(3)
A 		1.6
(-3)
A 		10.2
(1.7)
A 		11.6
(2.5)
P 		11
B 5.7
(-3)
A 		6.1
(-2.6)
A 		11.7
(3)
M 		7.7
(-1)
A 		6
C 18.9
(3)
A 		6.2
(-2.4)
A 		12.8
(0.4)
A 		4.7
(-3)
A 		14
avr 12.4
(3) 		4.6
(-3) 		11.6
(2.4) 		8
(-0.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 10.8
(-0.9)
P 		23.3
(2.2)
P 		2.3
(-3)
A 		5.1
(-2.3)
A 		26.5
(3)
P 		9
(-1.3)
A 		24
B 21.4
(2.3)
P 		19.4
(1.4)
P 		8.8
(-3)
A 		13.3
(-1.1)
P 		23.2
(3)
P 		14.5
(-0.6)
A 		14
C 17.4
(1.9)
P 		19.5
(3)
P 		8.2
(-3)
A 		10.9
(-1.6)
A 		11.1
(-1.5)
A 		9.7
(-2.2)
A 		11
avr 16.5
(1.2) 		20.7
(3) 		6.4
(-3) 		9.8
(-1.6) 		20.3
(2.8) 		11.1
(-1) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176220_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18923
Sequence Type Exemplar sequence
Representative Public ID Y71H2AM.C NCBI
Target Description Y71H2AM.C /REP_DB=WormBase Gene ID /WP=CE22941 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065219(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065219 NCBI Caenorhabditis elegans Y71H2AM.21 (Y71H2AM.21) mRNA, complete cds. 11/11 None
SNAP00000014451 ENSEMBL cdna:SNAP chromosome:CEL140:III:2789979:2795451:-1 10/11 None
GENEFINDER00000014485 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2793333:2800858:-1 10/11 None
Y71H2AM.21 ENSEMBL cdna:known chromosome:CEL140:III:2792778:2795429:-1 gene:Y71H2AM.21 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2792777-2795429(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.21
Entrez Gene 190613 Entrez gene
WormBase CE39071 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_497620 Y71H2AM.21 [Caenorhabditis elegans] 0.0
blast ABA00159 Hypothetical protein Y71H2AM.21 [Caenorhabditis elegans] 1.0E-175
blast CAE68368 Hypothetical protein CBG14123 [Caenorhabditis briggsae] 1.0E-164
blast AAC32864 putative potassium channel subunit n2P26 [Caenorhabditis elegans] 1.0E-163
blast AAK39200 Twik family of potassium channels protein 26 [Caenorhabditis elegans] ref|NP_508522.2| TWiK family of potassium channels family member (twk-26) [Caenorhabditis elegans] pir||T16629 hypothetical protein M02F4.5 - Caenorhabditis elegans 1.0E-163
blast NP_497620 Y71H2AM.21 [Caenorhabditis elegans] 1.0E-132
blast ABA00159 Hypothetical protein Y71H2AM.21 [Caenorhabditis elegans] 1.0E-132
blast CAE68368 Hypothetical protein CBG14123 [Caenorhabditis briggsae] 1.0E-61
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497620.1 2 109-131,141-163
Y71H2AM.21 2 109-131,141-163
SNAP00000014451 2 112-134,144-166
GENEFINDER00000014485 6 47-69,166-188,198-220,270-292,307-326,333-355
Sequence
Target Sequence 
>C. ELEGANS:176220_AT
gtgaacacttcaatctactctacgctttaccagagcttctataatttgatatatagtctc
gaggatcagctcgatcggattcataactcacggagaagaggtgcaggatatcgagttttt
caggatatggagtcaacatttcaaatgctcgtatgcacatttcctccatcaccggcacat
tctcgtattcgatttgcatccttaatacgagactcgttcagggaagccaagaaggaaagt
gcgcaaccaaaagtccgcacgagaaccgaatcagacatccccgacggattccaacaatgg
atggcgaaacgatcacgacgattacattctgcagacgatgaaatgaaggaaaaacgacga
gctccaactttaggagcactcggaggttttgatatgagcttgttcaagccacgaaaccga
tcaaccacttcaccattctgtcatactgacgaagaagtcgatactgatgaaagcgttcca
ttcatcctctgggaccactttccagtttgggctcggcgttgtggcgagccaattaaaatc
acgtggtgttcgccattca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAACACTTCAATCTACTCTACGC 49 483 475 Antisense
TACTCTACGCTTTACCAGAGCTTCT 138 647 490 Antisense
GCTCGATCGGATTCATAACTCACGG 598 299 543 Antisense
AAATGCTCGTATGCACATTTCCTCC 75 119 617 Antisense
GCAACCAAAAGTCCGCACGAGAACC 654 317 717 Antisense
ACGAGAACCGAATCAGACATCCCCG 614 93 733 Antisense
ATCCCCGACGGATTCCAACAATGGA 269 39 751 Antisense
GATCACGACGATTACATTCTGCAGA 263 421 785 Antisense
TCAAGCCACGAAACCGATCAACCAC 135 631 878 Antisense
CACTTCACCATTCTGTCATACTGAC 464 197 900 Antisense
AAAATCACGTGGTGTTCGCCATTCA 68 129 1009 Antisense
Sequence Source Affymetrix Proprietary Database