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[176217_at]

Description Y105E8B.UU /REP_DB=WormBase Gene ID /WP=CE23208 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 22      4grp_high_8_8 > 07      6grp_highest_nonorm > 74      6grp_total > 63      4grp_total > 0      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1012
1 2 3 4 range
A 559.5
(-2.2)
P 		441.9
(-3)
P 		590.2
(-2)
P 		1359.8
(3)
P 		918
B 583.2
(-2.2)
P 		454
(-3)
P 		580.3
(-2.2)
P 		1444.7
(3)
P 		991
C 564.3
(-2)
P 		432.9
(-3)
P 		742.7
(-0.6)
P 		1219.3
(3)
P 		786
avr 569
(-2.2) 		442.9
(-3) 		637.7
(-1.7) 		1341.3
(3) 		898

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 709.2
(0.2)
P 		596.9
(-2.9)
P 		808.9
(3)
P 		593.3
(-3)
P 		627.5
(-2)
P 		652.9
(-1.3)
P 		216
B 652.9
(-1.6)
P 		668.3
(-0.9)
P 		758.5
(3)
P 		670
(-0.9)
P 		646.7
(-1.9)
P 		620.7
(-3)
P 		138
C 520
(-3)
P 		671.1
(-0.3)
P 		682.5
(-0.1)
P 		851.8
(3)
P 		564.5
(-2.2)
P 		697.2
(0.2)
P 		332
avr 627.4
(-2.4) 		645.4
(-1.6) 		750
(3) 		705
(1) 		612.9
(-3) 		656.9
(-1.1) 		137
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 203.7
(3)
P 		95.9
(-3)
P 		154.3
(0.3)
P 		199.1
(2.7)
P 		108
B 186
(-0.7)
P 		112.1
(-3)
P 		134.5
(-2.3)
P 		307.9
(3)
P 		196
C 186.8
(-0.2)
P 		124.5
(-3)
P 		258.8
(3)
P 		205.6
(0.6)
P 		134
avr 192.2
(0.9) 		110.8
(-3) 		182.5
(0.4) 		237.5
(3) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 130.7
(-3)
P 		212.3
(3)
P 		176.2
(0.3)
P 		150.5
(-1.5)
P 		189.3
(1.3)
P 		159
(-0.9)
P 		82
B 227.2
(0.5)
P 		222.5
(0.2)
P 		184.2
(-2.5)
P 		177.5
(-3)
P 		262.7
(3)
P 		231.4
(0.8)
P 		85
C 192.8
(-0.9)
P 		204.8
(0.2)
P 		173.7
(-2.8)
P 		171.3
(-3)
P 		182.7
(-1.9)
P 		233.4
(3)
P 		62
avr 183.6
(-0.8) 		213.2
(3) 		178
(-1.5) 		166.4
(-3) 		211.6
(2.8) 		207.9
(2.3) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176217_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18827
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.UU NCBI
Target Description Y105E8B.UU /REP_DB=WormBase Gene ID /WP=CE23208 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061148(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061148 NCBI Caenorhabditis elegans Y54E5A.1 (Y54E5A.1) mRNA, complete cds. 11/11 A 
Y54E5A.1 ENSEMBL cdna:known chromosome:CEL140:I:14696791:14699832:-1 gene:Y54E5A.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14696846-14697944(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E5A.1
Entrez Gene 173327 Entrez gene
SwissProt Q9XWK1 EMBL-EBI
WormBase CE28791 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27381.1.S1_AT similar to sphingolipid C4-hydroxylase/delta 4-desaturase cfa
CHICKEN:GGA.10747.1.S1_S_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) gga
CHICKEN:GGAAFFX.23495.1.S1_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) gga
U133_X3P:HS.159643.0.A1_3P_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
HG-U133_PLUS_2:236496_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
HG-U133B:236496_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
HG-U95E:83944_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
MOE430A:1424549_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MOUSE430A_2:1424549_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MOUSE430_2:1424549_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MU11KSUBA:AA683962_S_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MG-U74AV2:101384_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MU19KSUBB:TC31871_AT Degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase (Degs2), mRNA mm
RG-U34A:RC_AI639085_AT similar to RIKEN cDNA 2210008A03 gene (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC70116 Hypothetical protein Y54E5A.1 [Caenorhabditis elegans] emb|CAA21659.2| Hypothetical protein Y54E5A.1 [Caenorhabditis elegans] ref|NP_493549.1| Y54E5A.1 [Caenorhabditis elegans] 0.0
blast CAE64141 Hypothetical protein CBG08757 [Caenorhabditis briggsae] 0.0
blast EAL34272 GA21524-PA [Drosophila pseudoobscura] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 4.6E-50
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493549.1 3 66-88,160-177,198-220
Y54E5A.1 3 66-88,160-177,198-220
Sequence
Target Sequence 
>C. ELEGANS:176217_AT
gaattcgtcgtaaatccggcaatgggtccgtatgctcgactgaaacgaaagccacgtgtc
gcacaggaattctatggaaattatcagttatttgaatatgttgaagggttcctccaccat
attggtgtctaccgccttcaaaagtttgcagttaacgtgtttgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCGTCGTAAATCCGGCAATGG 472 321 73 Antisense
TCCGGCAATGGGTCCGTATGCTCGA 107 595 87 Antisense
TCCGTATGCTCGACTGAAACGAAAG 60 595 99 Antisense
GAAACGAAAGCCACGTGTCGCACAG 502 357 114 Antisense
AAGCCACGTGTCGCACAGGAATTCT 241 149 121 Antisense
GTGTCGCACAGGAATTCTATGGAAA 652 485 128 Antisense
ATATGTTGAAGGGTTCCTCCACCAT 511 21 168 Antisense
AAGGGTTCCTCCACCATATTGGTGT 16 159 176 Antisense
CCACCATATTGGTGTCTACCGCCTT 308 271 186 Antisense
GTCTACCGCCTTCAAAAGTTTGCAG 477 465 199 Antisense
AAAGTTTGCAGTTAACGTGTTTGAT 242 123 213 Antisense
Sequence Source Affymetrix Proprietary Database