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[176216_at]

Description Y48G1BM.A /REP_DB=WormBase Gene ID /WP=CE26112 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 61      4grp_total > 17      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 28.8
(-2)
A 		12.5
(-3)
A 		45.3
(-1)
A 		112.1
(3)
A 		100
B 15.7
(-3)
A 		68.3
(3)
A 		21.4
(-2.3)
A 		56.1
(1.6)
A 		53
C 6.7
(-3)
A 		35.4
(-1.2)
A 		31.8
(-1.4)
A 		99.9
(3)
A 		93
avr 17.1
(-3) 		38.7
(-1.2) 		32.8
(-1.7) 		89.4
(3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 77.6
(2.9)
A 		5.9
(-2.9)
A 		33.8
(-0.6)
A 		25.9
(-1.3)
A 		78.8
(3)
A 		4.5
(-3)
A 		74
B 26.3
(-0.7)
A 		35.1
(0.1)
A 		63.7
(3)
A 		59.8
(2.6)
A 		3.7
(-3)
A 		14
(-2)
A 		60
C 10.9
(-3)
A 		49.7
(1.8)
A 		32.4
(-0.3)
A 		59.2
(3)
A 		13.2
(-2.7)
A 		20.8
(-1.8)
A 		48
avr 38.3
(1.3) 		30.2
(-0.1) 		43.3
(2.1) 		48.3
(3) 		31.9
(0.2) 		13.1
(-3) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 10.5
(0.4)
A 		2.7
(-3)
A 		11.9
(1)
A 		16.4
(3)
A 		14
B 5
(-3)
A 		16.9
(3)
A 		4.9
(-3)
A 		12
(0.6)
A 		12
C 2.2
(-3)
A 		10.2
(0.3)
A 		11.1
(0.7)
A 		16.8
(3)
A 		15
avr 5.9
(-3) 		9.9
(-0.4) 		9.3
(-0.8) 		15.1
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 14.3
(0.5)
A 		2.1
(-2.7)
A 		7.4
(-1.3)
A 		6.6
(-1.5)
A 		23.8
(3)
A 		1.1
(-3)
A 		23
B 9.2
(0.2)
A 		11.7
(1.3)
A 		15.5
(2.9)
A 		15.8
(3)
A 		1.5
(-3)
A 		5.2
(-1.4)
A 		14
C 4
(-3)
A 		15.2
(3)
A 		8.2
(-0.8)
A 		11.9
(1.2)
A 		4.3
(-2.8)
A 		7
(-1.4)
A 		11
avr 9.2
(1.1) 		9.7
(1.5) 		10.4
(2.1) 		11.4
(3) 		9.9
(1.7) 		4.4
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176216_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18860
Sequence Type Exemplar sequence
Representative Public ID Y48G1BM.A NCBI
Target Description Y48G1BM.A /REP_DB=WormBase Gene ID /WP=CE26112 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058306
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058306 NCBI Caenorhabditis elegans Y48G1BM.9 (Y48G1BM.9) mRNA, complete cds. A 
SNAP00000014672 ENSEMBL cdna:SNAP chromosome:CEL140:I:248624:256642:-1 None
Y48G1BM.9 ENSEMBL cdna:known chromosome:CEL140:I:254709:256606:-1 gene:Y48G1BM.9 A 
GENEFINDER00000014676 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:254709:256642:-1 None
SNAP00000028432 ENSEMBL cdna:SNAP chromosome:CEL140:II:13045028:13047150:-1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5482948-5485044(+) 58.16 58.16
chrI:253275-256606(-) 100.0 100.0
chrII:13044996-13047174(-) 62.49 62.49
Public Domain and Genome References
Gene Title
Ensembl Y48G1BM.9
Entrez Gene 190042 Entrez gene
SwissProt Q965U5 EMBL-EBI
WormBase CE33229 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68501 Hypothetical protein Y48G1BM.9 [Caenorhabditis elegans] ref|NP_490707.2| Y48G1BM.9 [Caenorhabditis elegans] 1.0E-110
blast AAK68501 Hypothetical protein Y48G1BM.9 [Caenorhabditis elegans] ref|NP_490707.2| Y48G1BM.9 [Caenorhabditis elegans] 9.0E-98
blast AAK68501 Hypothetical protein Y48G1BM.9 [Caenorhabditis elegans] ref|NP_490707.2| Y48G1BM.9 [Caenorhabditis elegans] 5.0E-96
blast AAK68500 Hypothetical protein Y48G1BM.8 [Caenorhabditis elegans] ref|NP_490705.1| Y48G1BM.8 [Caenorhabditis elegans] 7.0E-90
blast CAE74309 Hypothetical protein CBG22017 [Caenorhabditis briggsae] 1.0E-87
blast CAE74309 Hypothetical protein CBG22017 [Caenorhabditis briggsae] 8.0E-86
blast CAE74309 Hypothetical protein CBG22017 [Caenorhabditis briggsae] 2.0E-83
blast NP_490697 Y48G1BL.5 [Caenorhabditis elegans] gb|AAF60688.1| Hypothetical protein Y48G1BL.5 [Caenorhabditis elegans] 6.0E-44
Protein Domains
Database ID Description E-Value
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 0.75
Pfam IPR000082 EMBL-EBI SEA domain 1.3E-5
Pfam IPR000082 EMBL-EBI SEA domain 1.3E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490707.2 1 207-229
Y48G1BM.9 1 207-229
SNAP00000014672 1 205-227
GENEFINDER00000014676 1 205-227
SNAP00000028432 1 40-62
Sequence
Target Sequence 
>C. ELEGANS:176216_AT
gagaaacctctggccaaaaatcggtaattaaacaatcaaatcgaaaaacatttaaaaaaa
aacaacaaaaaatgatacagacttttaaatctcgttctctaaagatcgagctcggtgaag
aactggcgtccgtcgccaatgtcctttcgatcgcaatacgaatttccgtacctcaggcac
aagtcattgaagacgatgaggtgctcgcagacgccttgatgaatgtagacgtatggctga
ccgagccgacaaatgagatccccgatcctcgtctccgacatctgtttcacgtgcatcgga
ccgatgtagtcgaactttttggcccaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAAACCTCTGGCCAAAAATCGGT 386 391 965 Antisense
ACAGACTTTTAAATCTCGTTCTCTA 149 109 1041 Antisense
CGAGCTCGGTGAAGAACTGGCGTCC 308 251 1071 Antisense
CCAATGTCCTTTCGATCGCAATACG 162 277 1100 Antisense
GAATTTCCGTACCTCAGGCACAAGT 522 325 1124 Antisense
AAGACGATGAGGTGCTCGCAGACGC 570 153 1154 Antisense
GCTCGCAGACGCCTTGATGAATGTA 550 299 1167 Antisense
AATGTAGACGTATGGCTGACCGAGC 479 157 1186 Antisense
GACCGAGCCGACAAATGAGATCCCC 180 383 1203 Antisense
TCCGACATCTGTTTCACGTGCATCG 39 595 1238 Antisense
TGTAGTCGAACTTTTTGGCCCAACT 645 557 1269 Antisense
Sequence Source Affymetrix Proprietary Database