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[176205_at]

Description C29E4.10 /REP_DB=WormBase Gene ID /WP=CE26467 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 84      6grp_total > 75      4grp_total > 13      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 137.3
(1.3)
P 		54.5
(-3)
A 		101.7
(-0.5)
A 		169.2
(3)
P 		115
B 126.8
(0.4)
P 		95.7
(-1.5)
P 		69.2
(-3)
A 		171.9
(3)
P 		103
C 136.3
(0.8)
P 		97.2
(-1.1)
A 		59.5
(-3)
A 		179.5
(3)
P 		120
avr 133.5
(0.5) 		82.5
(-2.6) 		76.8
(-3) 		173.5
(3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 110.1
(-1.8)
P 		91
(-3)
P 		162.3
(1.5)
P 		150.3
(0.7)
P 		136.2
(-0.2)
P 		186.5
(3)
P 		96
B 113.5
(-1.3)
P 		102
(-3)
P 		102.5
(-2.9)
P 		128.8
(1)
P 		142.6
(3)
P 		137.1
(2.2)
P 		41
C 83.6
(-3)
P 		101.6
(-1.6)
M 		158.4
(3)
P 		135.2
(1.1)
P 		111.3
(-0.8)
P 		153.2
(2.6)
P 		75
avr 102.4
(-2.6) 		98.2
(-3) 		141.1
(1.2) 		138.1
(0.9) 		130
(0.1) 		158.9
(3) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 50
(3)
P 		11.8
(-3)
A 		26.6
(-0.7)
A 		24.8
(-1)
P 		38
B 40.4
(3)
P 		23.6
(-1.1)
P 		16
(-3)
A 		36.6
(2.1)
P 		24
C 45.1
(3)
P 		27.9
(-1.2)
A 		20.7
(-3)
A 		30.3
(-0.6)
P 		24
avr 45.2
(3) 		21.1
(-3) 		21.1
(-3) 		30.6
(-0.6) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 20.3
(-3)
P 		32.4
(-0.1)
P 		35.3
(0.6)
P 		38.1
(1.3)
P 		41.1
(2)
P 		45.4
(3)
P 		25
B 39.5
(-0.3)
P 		33.9
(-1.4)
P 		24.9
(-3)
P 		34.1
(-1.3)
P 		57.9
(3)
P 		51.1
(1.8)
P 		33
C 31
(-2.1)
P 		31
(-2.1)
M 		40.3
(0.3)
P 		27.2
(-3)
P 		36
(-0.8)
P 		51.3
(3)
P 		24
avr 30.3
(-3) 		32.4
(-2.3) 		33.5
(-2) 		33.1
(-2.1) 		45
(1.6) 		49.3
(3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176205_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8099
Sequence Type Exemplar sequence
Representative Public ID C29E4.10 NCBI
Target Description C29E4.10 /REP_DB=WormBase Gene ID /WP=CE26467 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066325(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066325 NCBI Caenorhabditis elegans C29E4.10 (C29E4.10) mRNA, complete cds. 11/11 None
GENEFINDER00000010843 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7937781:7940780:1 11/11 None
C29E4.10 ENSEMBL cdna:known chromosome:CEL140:III:7937781:7940671:1 gene:C29E4.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000010835 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7937778-7940669(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C29E4.10
Entrez Gene 183002 Entrez gene
SwissProt Q95QT2 EMBL-EBI
WormBase CE26467 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84495 Hypothetical protein C29E4.10 [Caenorhabditis elegans] ref|NP_498726.1| C29E4.10 [Caenorhabditis elegans] sp|Q95QT2|GALC_CAEEL Putative galactocerebrosidase precursor (GALCERase) (Galactosylceramidase) (Galactosylceramide beta-galactosidase) (Galactocerebroside beta-galactosidase) 0.0
blast NP_000144 galactosylceramidase precursor [Homo sapiens] dbj|BAA24902.1| galactocerebrosidase [Homo sapiens] sp|P54803|GALC_HUMAN Galactocerebrosidase precursor (GALCERase) (Galactosylceramidase) (Galactosylceramide beta-galactosidase) (Galactocerebroside beta-galactosidase) dbj|BAA04971.1| galactocerebrosidase [Homo sapiens] gb|AAA16645.1| galctocerebrosidase 2.0E-71
blast AAH36518 Galactosylceramidase, precursor [Homo sapiens] pir||I54205 galactosylceramidase (EC 3.2.1.46) precursor - human gb|AAA80975.1| galactocerebrosidase 6.0E-71
blast NP_001005921 galactosylceramidase like [Danio rerio] gb|AAH83517.1| Galactosylceramidase like [Danio rerio] 4.0E-32
blast XP_789829 PREDICTED: similar to galactosylceramidase like [Strongylocentrotus purpuratus] 5.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR001286 EMBL-EBI Glycoside hydrolase, family 59 1.7E-40
Pfam IPR001286 EMBL-EBI Glycoside hydrolase, family 59 1.4E-76
Sequence
Target Sequence 
>C. ELEGANS:176205_AT
tccacggccataccatgtcaagccaagattaaatatgtcattaaactttggaccttcaaa
gttcttttcaaaatctgtcaattctacaattaccattcttcttcctgttaattcatttgg
aatactcactacacttccagtttcaactccgaaaagagttactataaaaacaccattggt
tcctttgaattatcacgacgactttgaaaattatgaattcgacgaggagccaaagttttg
gatgccacaaaaaggatcatgggtggtacgaaatggacgggcggtacagaaagtgacacg
cccatcaattagttggtgcacctcacacattcgaactccatatgcagttatggctgacat
agaagtaataaactgtcgtggaatatttgttgagattgaattttcaaataaaaaagtaac
tatattttccgatttcgttgaccgttcaataattgctgaattcaaagcacgaagaaaagt
agaatttggaaagttttataaattctcaattcatttactggactcgcatctcttcataaa
attcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCACGGCCATACCATGTCAAGCCA 598 587 897 Antisense
TACAATTACCATTCTTCTTCCTGTT 601 641 981 Antisense
TTCATTTGGAATACTCACTACACTT 559 689 1008 Antisense
TACACTTCCAGTTTCAACTCCGAAA 329 643 1026 Antisense
GGACGGGCGGTACAGAAAGTGACAC 532 479 1171 Antisense
GTGACACGCCCATCAATTAGTTGGT 570 481 1189 Antisense
ATTAGTTGGTGCACCTCACACATTC 128 13 1204 Antisense
CACACATTCGAACTCCATATGCAGT 124 195 1220 Antisense
TTTCCGATTTCGTTGACCGTTCAAT 395 669 1322 Antisense
AATTCATTTACTGGACTCGCATCTC 180 177 1404 Antisense
GACTCGCATCTCTTCATAAAATTCG 352 375 1417 Antisense
Sequence Source Affymetrix Proprietary Database