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[176202_at]

Description Y69A2AR.B /REP_DB=WormBase Gene ID /WP=CE26558 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_high1_10_10 > 15      6grp_total > 15      4grp_total > 54      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 556
1 2 3 4 range
A 615.5
(3)
P 		298.2
(-0.9)
P 		218.3
(-1.9)
P 		125.5
(-3)
A 		490
B 559.3
(3)
P 		293.7
(-0.7)
P 		213.5
(-1.9)
P 		133.6
(-3)
A 		426
C 429.2
(3)
P 		311.4
(1.1)
P 		236.3
(-0.1)
P 		59.8
(-3)
A 		369
avr 534.7
(3) 		301.1
(-0.3) 		222.7
(-1.4) 		106.3
(-3) 		428

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 335.2
(0.1)
P 		516.4
(3)
P 		144.1
(-3)
P 		182.7
(-2.4)
P 		156
(-2.8)
M 		166.5
(-2.6)
A 		372
B 341.7
(1.4)
P 		438.5
(3)
P 		143.2
(-1.9)
P 		150.9
(-1.8)
P 		122.7
(-2.2)
M 		76.8
(-3)
M 		362
C 581.5
(3)
P 		341.4
(0.1)
P 		330
(-0.1)
P 		148.4
(-2.3)
A 		256.2
(-1)
P 		92.3
(-3)
P 		489
avr 419.5
(2.8) 		432.1
(3) 		205.8
(-1.2) 		160.7
(-2.1) 		178.3
(-1.8) 		111.9
(-3) 		320
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 224.1
(3)
P 		64.7
(-1.6)
P 		57.1
(-1.9)
P 		18.4
(-3)
A 		206
B 178.4
(3)
P 		72.5
(-1.2)
P 		49.5
(-2.2)
P 		28.5
(-3)
A 		150
C 142.1
(3)
P 		89.5
(0.6)
P 		82.4
(0.3)
P 		10.1
(-3)
A 		132
avr 181.5
(3) 		75.6
(-0.9) 		63
(-1.4) 		19
(-3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 187
1 2 3 4 5 6 range
A 61.8
(-1.8)
P 		183.7
(3)
P 		31.4
(-3)
P 		46.3
(-2.4)
P 		47.1
(-2.4)
M 		40.5
(-2.6)
A 		152
B 118.9
(1.6)
P 		146
(3)
P 		34.8
(-2.7)
P 		40
(-2.4)
P 		49.9
(-1.9)
M 		28.6
(-3)
M 		117
C 215.5
(3)
P 		104.2
(-0.6)
P 		84
(-1.2)
P 		29.8
(-3)
A 		82.9
(-1.3)
P 		30.9
(-3)
P 		186
avr 132.1
(2.3) 		144.6
(3) 		50.1
(-2.1) 		38.7
(-2.7) 		60
(-1.6) 		33.3
(-3) 		111
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176202_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19270
Sequence Type Exemplar sequence
Representative Public ID Y69A2AR.B NCBI
Target Description Y69A2AR.B /REP_DB=WormBase Gene ID /WP=CE26558 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067839(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067839 NCBI Caenorhabditis elegans Y69A2AR.31 (Y69A2AR.31) mRNA, complete cds. 10/11 A 
SNAP00000018824 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2635222:2655555:-1 10/11 None
GENEFINDER00000018840 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2650489:2663963:-1 11/11 None
Y69A2AR.31 ENSEMBL cdna:known chromosome:CEL140:IV:2650381:2664569:-1 gene:Y69A2AR.31 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2651709-2659348(-) 96.44 100.0
Public Domain and Genome References
Gene Title
Ensembl Y69A2AR.31
Entrez Gene 177049 Entrez gene
SwissProt Q95XG5 EMBL-EBI
WormBase CE27519 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68577 Hypothetical protein Y69A2AR.31 [Caenorhabditis elegans] ref|NP_500240.1| Y69A2AR.31 [Caenorhabditis elegans] 0.0
blast CAE68242 Hypothetical protein CBG13916 [Caenorhabditis briggsae] 0.0
blast AAK68576 Mad (yeast mitosis arrest deficient) related protein 2, isoform a [Caenorhabditis elegans] ref|NP_001023563.1| MAD (yeast Mitosis Arrest DeFicient) related family member (mdf-2) [Caenorhabditis elegans] gb|AAF63495.1| MDF-2 [Caenorhabditis elegans] 1.0E-97
blast XP_793677 PREDICTED: similar to glial cell line derived neurotrophic factor family receptor alpha 2, partial [Strongylocentrotus purpuratus] 1.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR003511 EMBL-EBI DNA-binding HORMA 3.9E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500240.1 1 12-34
Y69A2AR.31 1 12-34
GENEFINDER00000018840 2 1037-1059,1107-1129
Sequence
Target Sequence 
>C. ELEGANS:176202_AT
gagtctgaggatcatctcgcgtgtgaaccagtcccatgtctagaccatggtacttgtgat
gctccgctgactactattgaattcggcgagcgtgtattcctcaaggaccgtggagcctgc
tactgtgaaactggaaattttgtgtgcgaatttccagaagattcaccagagacctacccg
gggctctacatgtcaatcgggtactcacagaaagatgtggagctcattcgaaaagccgtg
ccacgcgaaattctcgataaagccggcttttcgtcgtccgatgcttccaccgatatcgca
tcacgaatgcaaatcgcttttgaacgtctgttgccgaaaaacttgcagtgccgaattgtg
acgatgaatgagctcagcgagcccggcaacgctttgtaccgtatcgagtggtacggtaga
aatgagatgttcaatcatacacgtaccaggtggcattctgatggtgctgagaaggtaggt
caaaaattttcgcccgctccggccagtctggactttttgcgtaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCTGAGGATCATCTCGCGTGTG 46 401 1717 Antisense
GTGTGAACCAGTCCCATGTCTAGAC 710 485 1737 Antisense
ATGCTCCGCTGACTACTATTGAATT 330 43 1775 Antisense
AATTCGGCGAGCGTGTATTCCTCAA 555 177 1796 Antisense
TATTCCTCAAGGACCGTGGAGCCTG 624 659 1811 Antisense
GTGGAGCCTGCTACTGTGAAACTGG 241 491 1826 Antisense
GGGCTCTACATGTCAATCGGGTACT 696 491 1897 Antisense
GATGCTTCCACCGATATCGCATCAC 44 413 1996 Antisense
AATCGCTTTTGAACGTCTGTTGCCG 44 169 2028 Antisense
GGCAACGCTTTGTACCGTATCGAGT 494 533 2101 Antisense
GCCAGTCTGGACTTTTTGCGTAACT 590 279 2218 Antisense
Sequence Source Affymetrix Proprietary Database