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[176200_at]

Description Y69A2AR.Y /REP_DB=WormBase Gene ID /WP=CE22803 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 73      4grp_total > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 26.4
(-1.7)
A 		15.2
(-3)
A 		26.9
(-1.6)
A 		66.8
(3)
A 		52
B 39.9
(0.4)
A 		9.9
(-3)
A 		21.3
(-1.7)
A 		62.8
(3)
A 		53
C 39.4
(-2.7)
A 		45.7
(-1.8)
A 		37.1
(-3)
A 		79
(3)
A 		42
avr 35.2
(-1.5) 		23.6
(-3) 		28.4
(-2.4) 		69.5
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 46.4
(0)
A 		42.2
(-0.3)
A 		45
(-0.1)
A 		7.3
(-3)
A 		84.3
(3)
A 		12.3
(-2.6)
A 		77
B 6.5
(-3)
A 		25.8
(-1.2)
A 		69.5
(3)
A 		59.7
(2.1)
A 		39.2
(0.1)
A 		42.5
(0.4)
A 		63
C 4.8
(-3)
A 		31.2
(-0.3)
A 		26.7
(-0.8)
A 		63.2
(3)
A 		22.5
(-1.2)
A 		28.6
(-0.6)
A 		58
avr 19.2
(-3) 		33.1
(-0.2) 		47.1
(2.7) 		43.4
(1.9) 		48.7
(3) 		27.8
(-1.3) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 9.6
(2.8)
A 		3.3
(-3)
A 		7
(0.4)
A 		9.8
(3)
A 		7
B 12.7
(2.6)
A 		2.4
(-3)
A 		4.9
(-1.6)
A 		13.4
(3)
A 		11
C 13
(-1.5)
A 		13.1
(-0)
A 		12.9
(-3)
A 		13.3
(3)
A 		0
avr 11.8
(2.6) 		6.3
(-3) 		8.3
(-1) 		12.2
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 8.6
(-1.3)
A 		15
(0.3)
A 		9.8
(-1)
A 		1.9
(-3)
A 		25.4
(3)
A 		3
(-2.7)
A 		24
B 2.3
(-3)
A 		8.6
(-0.4)
A 		16.9
(3)
A 		15.8
(2.5)
A 		15.9
(2.6)
A 		15.9
(2.6)
A 		15
C 1.8
(-3)
A 		9.5
(1.2)
A 		6.8
(-0.2)
A 		12.7
(3)
A 		7.3
(0)
A 		9.6
(1.3)
A 		11
avr 4.2
(-3) 		11
(0.4) 		11.2
(0.5) 		10.1
(-0.1) 		16.2
(3) 		9.5
(-0.4) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176200_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19266
Sequence Type Exemplar sequence
Representative Public ID Y69A2AR.Y NCBI
Target Description Y69A2AR.Y /REP_DB=WormBase Gene ID /WP=CE22803 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067827(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067827 NCBI Caenorhabditis elegans Y69A2AR.9 (Y69A2AR.9) mRNA, complete cds. 11/11 None
SNAP00000018816 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2585099:2601395:1 11/11 None
GENEFINDER00000018837 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2584774:2596813:1 11/11 None
Y69A2AR.9 ENSEMBL cdna:known chromosome:CEL140:IV:2594655:2596271:1 gene:Y69A2AR.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2594660-2596277(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y69A2AR.9
Entrez Gene 190535 Entrez gene
SwissProt Q95XH5 EMBL-EBI
WormBase CE22803 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04287 Hypothetical protein F35E2.6 [Caenorhabditis elegans] ref|NP_492984.1| F35E2.6 [Caenorhabditis elegans] 0.0
blast NP_500227 Y69A2AR.10 [Caenorhabditis elegans] 0.0
blast CAB04749 Hypothetical protein T22H2.2 [Caenorhabditis elegans] ref|NP_492983.1| T22H2.2 [Caenorhabditis elegans] 0.0
blast CAB04285 Hypothetical protein F35E2.7 [Caenorhabditis elegans] ref|NP_492985.1| F35E2.7 [Caenorhabditis elegans] 1.0E-176
blast NP_500227 Y69A2AR.10 [Caenorhabditis elegans] 1.0E-169
blast NP_505161 F38E1.2 [Caenorhabditis elegans] 1.0E-152
blast AAK68583 Hypothetical protein Y69A2AR.9 [Caenorhabditis elegans] ref|NP_500228.1| Y69A2AR.9 [Caenorhabditis elegans] 1.0E-120
blast AAF59585 Hypothetical protein Y54G2A.13 [Caenorhabditis elegans] ref|NP_500256.2| Y54G2A.13 [Caenorhabditis elegans] 1.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 7.7E-79
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 3.7E-4
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 1.5E-16
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 1.2E-28
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 1.9E-58
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 5.9E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500228.1 1 235-257
Y69A2AR.9 1 235-257
SNAP00000018816 5 49-71,143-162,223-240,247-269,1452-1474
GENEFINDER00000018837 9 15-32,39-58,95-117,166-185,215-237,246-263,278-293,360-382,1728-1750
Sequence
Target Sequence 
>C. ELEGANS:176200_AT
tcatctcctgactgtttaaccacgattgctccatctgaagacaactccaccactactgca
tcctcgactgaagccaccacattctcgacaactacttccgacgagaagacctcaacgaca
gagaagacaaatccaggaccagcaccaggacccggaccaggaccctttgcctcctcgcca
tctccaaaggatgtcacagcggaagcaccgactcctcctaagactacgaagactacacaa
acgccaggtcgaacactgccgccggaagaaaggcaagaagaaactgatcctcttgcgaat
ggcccatcgaacatcgaatctgacgaggtgtacagcaatatgacttgttcgatgaacgtc
gacatttgtgatgagaattactgtaagaaacttgctgctggaaggaagaaggctgacagt
ggtgatggtggtgttcagttgtatctctggattggtctagcttttctcctggcaataaat
gttggactttttgctaaaaactacaagaagtacttcaagaagaagcgagctgctccggtg
act

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATCTCCTGACTGTTTAACCACGA 433 619 289 Antisense
TTAACCACGATTGCTCCATCTGAAG 362 677 304 Antisense
TCCTCGCCATCTCCAAAGGATGTCA 331 591 460 Antisense
ATGTCACAGCGGAAGCACCGACTCC 655 47 479 Antisense
CGACTCCTCCTAAGACTACGAAGAC 612 249 497 Antisense
CACAAACGCCAGGTCGAACACTGCC 521 193 524 Antisense
GAAACTGATCCTCTTGCGAATGGCC 341 357 568 Antisense
GCGAATGGCCCATCGAACATCGAAT 274 289 583 Antisense
GTATCTCTGGATTGGTCTAGCTTTT 289 455 729 Antisense
TCTAGCTTTTCTCCTGGCAATAAAT 177 615 744 Antisense
GAAGAAGCGAGCTGCTCCGGTGACT 159 341 807 Antisense
Sequence Source Affymetrix Proprietary Database