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[176177_at]

Description Y38F2AR.A /REP_DB=WormBase Gene ID /WP=CE21626 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 18      4grp_total > 42      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 80
(-1.5)
A 		135.5
(3)
A 		93.3
(-0.4)
A 		61.6
(-3)
A 		74
B 49.7
(-3)
A 		118.8
(3)
A 		77.9
(-0.6)
A 		63.1
(-1.8)
A 		69
C 69.1
(-3)
A 		93.4
(0.5)
A 		94.4
(0.7)
A 		110.6
(3)
A 		42
avr 66.3
(-3) 		115.9
(3) 		88.5
(-0.3) 		78.4
(-1.5) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 101.4
(2)
A 		116.6
(3)
A 		69.7
(-0.2)
A 		88.9
(1.1)
A 		29.7
(-3)
A 		97.2
(1.7)
A 		87
B 97.1
(3)
A 		88.3
(2.3)
A 		25.8
(-3)
A 		94
(2.7)
A 		72.9
(1)
A 		67.2
(0.5)
A 		71
C 150
(3)
A 		27.6
(-3)
A 		100.4
(0.6)
A 		40.9
(-2.3)
A 		104.9
(0.8)
A 		40.1
(-2.4)
A 		122
avr 116.2
(3) 		77.5
(-1.6) 		65.3
(-3) 		74.6
(-1.9) 		69.2
(-2.5) 		68.2
(-2.7) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 29.1
(2.9)
A 		29.4
(3)
A 		24.4
(1.5)
A 		9
(-3)
A 		20
B 15.9
(-2.1)
A 		29.4
(3)
A 		18.1
(-1.2)
A 		13.4
(-3)
A 		16
C 22.9
(-1.2)
A 		26.8
(0.4)
A 		32.9
(3)
A 		18.6
(-3)
A 		14
avr 22.6
(0.6) 		28.5
(3) 		25.1
(1.6) 		13.7
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 18.7
(-1.2)
A 		41.5
(3)
A 		15.2
(-1.9)
A 		22.5
(-0.5)
A 		9
(-3)
A 		23.7
(-0.3)
A 		33
B 33.8
(3)
A 		29.4
(2)
A 		6.3
(-3)
A 		24.9
(1.1)
A 		29.6
(2.1)
A 		25.1
(1.1)
A 		28
C 55.6
(3)
A 		8.4
(-3)
A 		25.5
(-0.8)
A 		8.2
(-3)
A 		34
(0.3)
A 		13.4
(-2.3)
A 		47
avr 36
(3) 		26.4
(0.2) 		15.7
(-3) 		18.5
(-2.2) 		24.2
(-0.5) 		20.7
(-1.5) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176177_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19254
Sequence Type Exemplar sequence
Representative Public ID Y38F2AR.A NCBI
Target Description Y38F2AR.A /REP_DB=WormBase Gene ID /WP=CE21626 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067801(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067801 NCBI Caenorhabditis elegans Y38F2AR.12 (Y38F2AR.12) mRNA, complete cds. 11/11 None
SNAP00000033670 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2407339:2411000:-1 11/11 None
GENEFINDER00000033676 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2399492:2412408:-1 11/11 None
Y38F2AR.12 ENSEMBL cdna:known chromosome:CEL140:IV:2408028:2412408:-1 gene:Y38F2AR.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2408033-2412414(-) 99.14 100.0
Public Domain and Genome References
Gene Title
Ensembl Y38F2AR.12
Entrez Gene 189679 Entrez gene
SwissProt Q95XR7 EMBL-EBI
WormBase CE21626 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_500202 Y38F2AR.12 [Caenorhabditis elegans] gb|AAK68442.1| Hypothetical protein Y38F2AR.12 [Caenorhabditis elegans] 0.0
blast CAE70292 Hypothetical protein CBG16813 [Caenorhabditis briggsae] 0.0
blast NP_694762 5-oxoprolinase (ATP-hydrolysing) [Mus musculus] gb|AAH34522.1| 5-oxoprolinase (ATP-hydrolysing) [Mus musculus] sp|Q8K010|OPLA_MOUSE 5-oxoprolinase (5-oxo-L-prolinase) (Pyroglutamase) (5-OPase) 0.0
blast AAH85330 Oplah protein [Rattus norvegicus] sp|P97608|OPLA_RAT 5-oxoprolinase (5-oxo-L-prolinase) (Pyroglutamase) (5-OPase) 0.0
blast CAE70292 Hypothetical protein CBG16813 [Caenorhabditis briggsae] 1.0E-177
blast CAE70292 Hypothetical protein CBG16813 [Caenorhabditis briggsae] 1.0E-166
blast CAF97977 unnamed protein product [Tetraodon nigroviridis] 1.0E-138
Protein Domains
Database ID Description E-Value
Pfam IPR008040 EMBL-EBI Hydantoinaseoxoprolinase, N-terminal 2.5E-92
Pfam IPR002821 EMBL-EBI Hydantoinase/oxoprolinase 3.2E-61
Pfam IPR002821 EMBL-EBI Hydantoinase/oxoprolinase 7.7E-31
Pfam IPR002821 EMBL-EBI Hydantoinase/oxoprolinase 1.0E-126
Pfam IPR002821 EMBL-EBI Hydantoinase/oxoprolinase 1.0E-126
Pfam IPR003692 EMBL-EBI Hydantoinase B/oxoprolinase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000033676 1 1271-1288
Sequence
Target Sequence 
>C. ELEGANS:176177_AT
tgcgagcacggcgtacaaggcatccgataagaagcctgttatcggattcgacatgggcgg
cacctcaaccgacgtgtgtagatactctggatacttggagcatgtcatggagactaccac
agctggcatcacaattcaggcgccacaactcgacattcgtacggtggccgctggtggagg
ttctaggcttttcttccgcgatggcctattgattgtgggccccgagtcggcgagtgctca
tccaggaccggttgccctcggcttcctcgctgtggccaatgaggaaatgtgccgcccaat
ccgtactctaacccaatcccgtggcttcaacccatctgagcatgtgctcgcatgtttcgg
tggagccggtggacaacacgcgtgtgccgtcgcgaaagttctggggatcagccaagttcg
gattcataagtacgcctcgttgctttcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCGAGCACGGCGTACAAGGCATCC 671 581 903 Antisense
AGACTACCACAGCTGGCATCACAAT 308 71 1013 Antisense
GCCACAACTCGACATTCGTACGGTG 482 277 1044 Antisense
GGCCGCTGGTGGAGGTTCTAGGCTT 177 537 1068 Antisense
TTCTTCCGCGATGGCCTATTGATTG 482 689 1093 Antisense
CAATGAGGAAATGTGCCGCCCAATC 645 177 1179 Antisense
CTTCAACCCATCTGAGCATGTGCTC 279 221 1227 Antisense
GTGCTCGCATGTTTCGGTGGAGCCG 696 477 1246 Antisense
TGCCGTCGCGAAAGTTCTGGGGATC 680 585 1287 Antisense
GGGGATCAGCCAAGTTCGGATTCAT 63 499 1305 Antisense
CATAAGTACGCCTCGTTGCTTTCAG 632 211 1327 Antisense
Sequence Source Affymetrix Proprietary Database