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[176174_at]

Description Y71H2AR.A /REP_DB=WormBase Gene ID /WP=CE22930 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 98      4grp_total > 15      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 16.9
(3)
A 		7.5
(-3)
A 		15.5
(2.1)
A 		8.9
(-2.1)
A 		9
B 39.4
(3)
A 		5.7
(-3)
A 		6.2
(-2.9)
A 		36.4
(2.5)
A 		34
C 47.9
(1.2)
P 		4.8
(-3)
A 		20.6
(-1.5)
A 		66.6
(3)
A 		62
avr 34.7
(2.5) 		6
(-3) 		14.1
(-1.4) 		37.3
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 77.2
(3)
A 		16.4
(-3)
A 		22
(-2.4)
A 		53.3
(0.6)
A 		75.8
(2.9)
A 		75.4
(2.8)
M 		61
B 19.3
(-2.9)
A 		23.5
(-2.5)
A 		19.2
(-2.9)
A 		17.5
(-3)
A 		96.7
(3)
A 		81.4
(1.8)
A 		79
C 6.5
(-3)
A 		28.3
(-1.2)
A 		29.9
(-1)
A 		29.7
(-1.1)
A 		47.2
(0.4)
A 		78.3
(3)
P 		72
avr 34.3
(-1.8) 		22.7
(-3) 		23.7
(-2.9) 		33.5
(-1.8) 		73.2
(2.4) 		78.4
(3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 6.1
(3)
A 		1.6
(-2.6)
A 		4.1
(0.5)
A 		1.3
(-3)
A 		5
B 12.6
(3)
A 		1.4
(-3)
A 		1.4
(-3)
A 		7.8
(0.4)
A 		11
C 15.9
(3)
P 		1.4
(-3)
A 		7.2
(-0.6)
A 		11.2
(1.1)
A 		15
avr 11.5
(3) 		1.5
(-3) 		4.2
(-1.4) 		6.8
(0.2) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 14.2
(0.1)
A 		5.8
(-2.7)
A 		4.8
(-3)
A 		13.5
(-0.1)
A 		22.9
(3)
A 		18.4
(1.5)
M 		18
B 6.7
(-2.6)
A 		7.8
(-2.4)
A 		4.7
(-3)
A 		4.6
(-3)
A 		39.3
(3)
A 		30.4
(1.5)
A 		35
C 2.4
(-3)
A 		8.6
(-1.4)
A 		7.6
(-1.7)
A 		6
(-2.1)
A 		15.3
(0.3)
A 		26.2
(3)
P 		24
avr 7.8
(-2.4) 		7.4
(-2.5) 		5.7
(-3) 		8
(-2.3) 		25.8
(3) 		25
(2.8) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176174_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18970
Sequence Type Exemplar sequence
Representative Public ID Y71H2AR.A NCBI
Target Description Y71H2AR.A /REP_DB=WormBase Gene ID /WP=CE22930 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065226(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065226 NCBI Caenorhabditis elegans Y71H2AR.2 (Y71H2AR.2) mRNA, complete cds. 11/11 None
Y71H2AR.2 ENSEMBL cdna:known chromosome:CEL140:III:2838753:2840917:-1 gene:Y71H2AR.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014454 4/11 Cross Hyb Matching Probes None
GENEFINDER00000014461 3/11 Cross Hyb Matching Probes None
GENEFINDER00000032450 8/11 Cross Hyb Matching Probes None
SNAP00000032447 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2838752-2840917(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AR.2
Entrez Gene 190615 Entrez gene
SwissProt Q9BL26 EMBL-EBI
WormBase CE22930 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4182 carboxypeptidase A activity inferred from electronic annotation QuickGO AmiGO
8234 cysteine-type peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29985 Hypothetical protein Y71H2AR.2 [Caenorhabditis elegans] ref|NP_497627.1| Y71H2AR.2 [Caenorhabditis elegans] 0.0
blast AAK29976 Hypothetical protein Y71H2AM.3 [Caenorhabditis elegans] ref|NP_497622.1| Y71H2AM.3 [Caenorhabditis elegans] 1.0E-104
blast AAB71030 Hypothetical protein K02E7.10 [Caenorhabditis elegans] ref|NP_493904.1| K02E7.10 [Caenorhabditis elegans] 3.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 1.2E-49
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497627.1 1 38-60
Y71H2AR.2 1 38-60
Sequence
Target Sequence 
>C. ELEGANS:176174_AT
gaatgctattggatacctcgccactcatggcatagaaacagaagcagactatccatatgt
tgacaagacaaatgaaaagtgtacctttgactcaactaaatcaaaaattcatttaaaaaa
aggagttgtggcagagggtaatgaagtactggggaaagtttatgtcacaaattatggacc
cgcgtttttcacgatgcgcgcccctccatccctctacgattataaaatcggaatctacaa
cccatccattgaagaatgtacaagtacacatgaaatccgatcaatggtaatcgttgggta
cggtatcgagggagagcaaaaatattggattgtgaagggctcattcgggacatcgtgggg
agagcagggatatatgaagttggcaagagatgttaatgcatgtgcaatggcaactacaat
tgcagttcttaccgaaatttttcttcgggtacttgtttcaaatttttgctgcataagttt
gaccgtacttttcaagatgtaccataaccaatcatttaaatgtttttccggagaacaccc
aatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGCTATTGGATACCTCGCCACT 451 331 468 Antisense
ATACCTCGCCACTCATGGCATAGAA 479 23 480 Antisense
GAAAAGTGTACCTTTGACTCAACTA 632 353 541 Antisense
ATTATGGACCCGCGTTTTTCACGAT 663 1 638 Antisense
GGTAATCGTTGGGTACGGTATCGAG 228 493 753 Antisense
TGTGAAGGGCTCATTCGGGACATCG 427 555 798 Antisense
CGGGACATCGTGGGGAGAGCAGGGA 9 243 813 Antisense
GCAACTACAATTGCAGTTCTTACCG 58 321 877 Antisense
GGGTACTTGTTTCAAATTTTTGCTG 24 489 914 Antisense
TTTTGCTGCATAAGTTTGACCGTAC 215 677 931 Antisense
TGTTTTTCCGGAGAACACCCAATAT 182 561 988 Antisense
Sequence Source Affymetrix Proprietary Database