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[176153_at]

Description Y41D4B.I /REP_DB=WormBase Gene ID /WP=CE26092 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 48      4grp_high_8_8 > 43      6grp_highest_nonorm > 90      6grp_total > 39      4grp_total > 52      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 193.2
(2.4)
P 		199.1
(3)
P 		180.7
(1)
P 		142.6
(-3)
P 		57
B 180.7
(3)
P 		156.7
(1.1)
P 		165.4
(1.8)
P 		104.8
(-3)
P 		76
C 192.9
(2.6)
P 		202.3
(3)
P 		146.9
(0.5)
P 		70
(-3)
A 		132
avr 188.9
(3) 		186
(2.8) 		164.3
(1.2) 		105.8
(-3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 150.8
(-0.2)
P 		174.1
(0.7)
P 		75.6
(-3)
P 		129.5
(-1)
P 		101.4
(-2)
P 		237.4
(3)
P 		162
B 144.5
(2.1)
P 		137.2
(1.5)
P 		80
(-2.5)
P 		72.8
(-3)
P 		124
(0.6)
P 		157.8
(3)
P 		85
C 291.2
(3)
P 		79.8
(-3)
P 		147.7
(-1.1)
P 		91.9
(-2.7)
P 		188.4
(0.1)
P 		125.3
(-1.7)
P 		211
avr 195.5
(3) 		130.4
(-1) 		101.1
(-2.8) 		98.1
(-3) 		137.9
(-0.5) 		173.5
(1.6) 		97
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 70.3
(3)
P 		43.2
(-0.3)
P 		47.2
(0.2)
P 		20.9
(-3)
P 		49
B 57.6
(3)
P 		38.7
(-0.2)
P 		38.3
(-0.3)
P 		22.3
(-3)
P 		35
C 63.8
(3)
P 		58.2
(2.4)
P 		51.2
(1.5)
P 		11.8
(-3)
A 		52
avr 63.9
(3) 		46.7
(0.7) 		45.6
(0.6) 		18.3
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 27.8
(-1.5)
P 		61.9
(3)
P 		16.5
(-3)
P 		32.8
(-0.8)
P 		30.6
(-1.1)
P 		57.8
(2.5)
P 		45
B 50.3
(1.7)
P 		45.7
(1)
P 		19.4
(-3)
P 		19.3
(-3)
P 		50.4
(1.7)
P 		58.8
(3)
P 		40
C 107.9
(3)
P 		24.3
(-2.6)
P 		37.6
(-1.7)
P 		18.5
(-3)
P 		61
(-0.1)
P 		41.9
(-1.4)
P 		89
avr 62
(3) 		44
(0.2) 		24.5
(-2.8) 		23.5
(-3) 		47.3
(0.7) 		52.8
(1.6) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176153_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19281
Sequence Type Exemplar sequence
Representative Public ID Y41D4B.I NCBI
Target Description Y41D4B.I /REP_DB=WormBase Gene ID /WP=CE26092 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067701(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067701 NCBI Caenorhabditis elegans Nuclear Pore complex Protein family member (npp-8) (npp-8) mRNA, complete cds. 9/11 A 
SNAP00000005510 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1568312:1585872:-1 11/11 None
GENEFINDER00000005551 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1569032:1572456:-1 9/11 None
Y41D4B.19b ENSEMBL cdna:known chromosome:CEL140:IV:1567919:1595822:-1 gene:Y41D4B.19 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000005540 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176966 2 1 NM_067701 174671_at (11), 176088_at (11), 176153_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1572099-1576109(-) 99.22 100.0
Public Domain and Genome References
Ensembl Y41D4B.19
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68470 Nuclear pore complex protein protein 8, isoform b [Caenorhabditis elegans] ref|NP_500102.3| Nuclear Pore complex Protein family member (npp-8) [Caenorhabditis elegans] 0.0
blast AAK68469 Nuclear pore complex protein protein 8, isoform a [Caenorhabditis elegans] ref|NP_500104.1| Nuclear Pore complex Protein family member (npp-8) [Caenorhabditis elegans] 1.0E-176
blast CAE67963 Hypothetical protein CBG13567 [Caenorhabditis briggsae] 1.0E-154
blast NP_956450 nucleoporin 155 [Danio rerio] gb|AAH45339.1| Nucleoporin 155 [Danio rerio] 6.0E-67
blast AAH47162 Nup155-prov protein [Xenopus laevis] 2.0E-65
blast AAK68470 Nuclear pore complex protein protein 8, isoform b [Caenorhabditis elegans] ref|NP_500102.3| Nuclear Pore complex Protein family member (npp-8) [Caenorhabditis elegans] 4.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR004870 EMBL-EBI Non-repetitive/WGA-negative nucleoporin 2.9E-38
Pfam IPR004870 EMBL-EBI Non-repetitive/WGA-negative nucleoporin 3.5E-8
Pfam IPR004870 EMBL-EBI Non-repetitive/WGA-negative nucleoporin 6.1E-10
Pfam IPR004870 EMBL-EBI Non-repetitive/WGA-negative nucleoporin 4.9E-39
Pfam IPR004870 EMBL-EBI Non-repetitive/WGA-negative nucleoporin 6.1E-10
Sequence
Target Sequence 
>C. ELEGANS:176153_AT
gtttatcgagttcttcctggtacaggagatcaacgctggccgcgggcaaaaatatttcga
tctgttgtggaggttctatgagaagagcggaaattatgataaagccgcaaggcttttgag
caaattggctgaaaatgataaagaagctttagataatacattatttctgcttcaaaaaat
ctggaaaatgggtttgacccaacgatgcgcctacctctcccacgcaattctatgtgctca
atcttgtaaggattcaactgtaactacaaatattgatgagctccgtgatcggctcgacgt
ggcaaatgttcaaatgagaattaaagatgctctcggctgctcggcgtcggcgtcggctcg
aaatcaggaattcgtcagaaaactcgatgggccgattctttcactgcaagagttgcttct
tcaatacgttgtaccatttaagctgcacaaaatcaagctctcactgctccattgtgctgg
aat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTATCGAGTTCTTCCTGGTACAG 81 449 360 Antisense
GGTACAGGAGATCAACGCTGGCCGC 87 505 378 Antisense
ATGGGTTTGACCCAACGATGCGCCT 58 51 547 Antisense
TCTCCCACGCAATTCTATGTGCTCA 415 611 575 Antisense
TGATGAGCTCCGTGATCGGCTCGAC 115 567 633 Antisense
TCGGCTCGACGTGGCAAATGTTCAA 474 607 648 Antisense
GAATTAAAGATGCTCTCGGCTGCTC 334 325 677 Antisense
TCGGCGTCGGCTCGAAATCAGGAAT 431 603 706 Antisense
AAACTCGATGGGCCGATTCTTTCAC 123 127 739 Antisense
GTTGCTTCTTCAATACGTTGTACCA 267 439 771 Antisense
TCTCACTGCTCCATTGTGCTGGAAT 372 613 818 Antisense
Sequence Source Affymetrix Proprietary Database