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[176147_s_at]

Description F10E9.8 /REP_DB=WormBase Gene ID /WP=CE00557 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 68      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 81      6grp_total > 68      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 29      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 247
1 2 3 4 range
A 279.2
(0.2)
P 		179.6
(-2.3)
P 		149
(-3)
P 		395.6
(3)
P 		247
B 346.6
(3)
P 		164.5
(-3)
P 		196.6
(-1.9)
P 		256.6
(0)
P 		182
C 342.3
(3)
P 		219.5
(-1.3)
P 		172.7
(-3)
P 		255.1
(-0.1)
P 		170
avr 322.7
(3) 		187.9
(-2.4) 		172.8
(-3) 		302.4
(2.2) 		150

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 215
1 2 3 4 5 6 range
A 277
(-1.4)
P 		314
(-0.2)
P 		223.3
(-3)
P 		258.1
(-1.9)
P 		237
(-2.6)
P 		419.3
(3)
P 		196
B 254.7
(-0.9)
P 		315.6
(1.6)
P 		218.8
(-2.4)
P 		204.6
(-3)
P 		234.4
(-1.8)
P 		349
(3)
P 		144
C 277.7
(-3)
P 		326.8
(-0.4)
P 		291.7
(-2.3)
P 		316.8
(-1)
P 		345.5
(0.5)
P 		393.2
(3)
P 		116
avr 269.8
(-1.9) 		318.8
(0.1) 		244.6
(-3) 		259.8
(-2.4) 		272.3
(-1.8) 		387.2
(3) 		143
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 101.6
(3)
P 		39
(-3)
P 		38.9
(-3)
P 		57.9
(-1.2)
P 		63
B 110.5
(3)
P 		40.6
(-3)
P 		45.6
(-2.6)
P 		54.7
(-1.8)
P 		70
C 113.3
(3)
P 		63.1
(-1.3)
P 		60.2
(-1.5)
P 		43
(-3)
P 		70
avr 108.5
(3) 		47.6
(-3) 		48.2
(-2.9) 		51.9
(-2.6) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 51.1
(-2.8)
P 		111.7
(3)
P 		48.6
(-3)
P 		65.5
(-1.4)
P 		71.5
(-0.8)
P 		102.1
(2.1)
P 		63
B 88.6
(-0.2)
P 		105
(1)
P 		53.1
(-3)
P 		54.2
(-2.9)
P 		95.2
(0.3)
P 		130.2
(3)
P 		77
C 102.9
(0.5)
P 		99.7
(0.2)
P 		74.2
(-2.1)
P 		63.7
(-3)
P 		111.8
(1.3)
P 		131.6
(3)
P 		68
avr 80.9
(-0.9) 		105.5
(1.5) 		58.6
(-3) 		61.1
(-2.8) 		92.8
(0.3) 		121.3
(3) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176147_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7742
Sequence Type Exemplar sequence
Representative Public ID F10E9.8 NCBI
Target Description F10E9.8 /REP_DB=WormBase Gene ID /WP=CE00557 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066427(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066427 NCBI Caenorhabditis elegans Spindle ASsembly abnormal family member (sas-4) (sas-4) mRNA, complete cds. 11/11 None
SNAP00000040910 ENSEMBL cdna:SNAP chromosome:CEL140:III:8314491:8318496:-1 11/11 None
GENEFINDER00000040917 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8314491:8316033:-1 11/11 None
F10E9.8 ENSEMBL cdna:known chromosome:CEL140:III:8314302:8318496:-1 gene:F10E9.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8314486-8321583(-) 98.76 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sas-4
Ensembl F10E9.8
Entrez Gene 176172 Entrez gene
SwissProt P34402 EMBL-EBI
WormBase CE29756 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK93847 Spindle assembly abnormal protein 4 [Caenorhabditis elegans] ref|NP_498828.2| Spindle ASsembly abnormal family member (sas-4) [Caenorhabditis elegans] sp|P34402|SAS4_CAEEL Spindle assembly abnormal protein 4 emb|CAD62434.1| SAS-4 protein [Caenorhabditis elegans] 0.0
blast S44800 F10E9.8 protein - Caenorhabditis elegans 0.0
blast Q60JJ0 Spindle assembly abnormal protein 1.0E-156
blast AAK93847 Spindle assembly abnormal protein 4 [Caenorhabditis elegans] ref|NP_498828.2| Spindle ASsembly abnormal family member (sas-4) [Caenorhabditis elegans] sp|P34402|SAS4_CAEEL Spindle assembly abnormal protein 4 emb|CAD62434.1| SAS-4 protein [Caenorhabditis elegans] 1.0E-133
Protein Domains
Database ID Description E-Value
scop a.2.7.Methicillin resistance protein FemA probable tRNA-binding arm All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Methicillin resistance protein FemA probable tRNA-binding arm 2.29999995231628
Sequence
Target Sequence 
>C. ELEGANS:176147_S_AT
atagctaacggtcttcttttcgaatattccaatggagatcttcgatgggttaatcggcag
aacgctgttaatatctacatatccgcagttgataaaacagtcagaattgatctccccaca
tacaatatttcaattattcatacatttcaaaggcaagttgaagtacttcgtcctggaaat
aacataacattgataagtattaaacgacgagaagttcgaactgatttgatttatcaaaac
ggaatgtataaaactgaaatcttcaatagggacggaagatatgttacgaaggattttagc
aatcaagaagtttcgagaaaatacaatcccggtacacacacatatcgcgacaatcaatgt
cgctacgttctcgtcactgattacaacgattttgagctcgttgagccagaattccgtctt
cgttggtatcagggagatccgactggtctcaacaatcagtatattctcaagatcattgga
cgacctgaatgcagcgagaaaacattgagacttgaagtgaatctttccacgtgtgaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAGCTAACGGTCTTCTTTTCGAAT 178 5 3028 Antisense
GATGGGTTAATCGGCAGAACGCTGT 207 407 3071 Antisense
AACAGTCAGAATTGATCTCCCCACA 385 135 3123 Antisense
GATCTCCCCACATACAATATTTCAA 46 419 3136 Antisense
CAATCCCGGTACACACACATATCGC 136 181 3351 Antisense
ACAATCAATGTCGCTACGTTCTCGT 195 115 3377 Antisense
GCTACGTTCTCGTCACTGATTACAA 459 303 3389 Antisense
GAGCTCGTTGAGCCAGAATTCCGTC 508 385 3421 Antisense
GCCAGAATTCCGTCTTCGTTGGTAT 67 277 3432 Antisense
GGGAGATCCGACTGGTCTCAACAAT 211 495 3459 Antisense
GAAGTGAATCTTTCCACGTGTGAAG 655 335 3541 Antisense
Sequence Source Affymetrix Proprietary Database