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[176145_at]

Description K08D12.3 /REP_DB=WormBase Gene ID /WP=CE21045 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 91      4grp_highest_8_8 > 01      4grp_high_8_8 > 54      6grp_highest_nonorm > 47      6grp_total > 92      4grp_total > 58      6grp_highest_sub > 05      6grp_high1_sub > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1432
1 2 3 4 range
A 5890
(-2.4)
P 		6794.5
(3)
P 		6664
(2.2)
P 		5794.2
(-3)
P 		1000
B 6145.9
(-0.8)
P 		6276.8
(-0.3)
P 		7056.2
(3)
P 		5624.5
(-3)
P 		1432
C 6009.1
(-1.9)
P 		6637.2
(3)
P 		5872.5
(-3)
P 		6098.9
(-1.2)
P 		765
avr 6015
(-1.6) 		6569.5
(3) 		6530.9
(2.7) 		5839.2
(-3) 		730

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1872
1 2 3 4 5 6 range
A 3098
(-1.6)
P 		3214.7
(-0.4)
P 		2984.1
(-2.8)
P 		3439.9
(2)
P 		3540.1
(3)
P 		2961.8
(-3)
P 		578
B 3145.7
(-1.6)
P 		3159.7
(-1.6)
P 		3641
(0.2)
P 		3367.3
(-0.8)
P 		4373.1
(3)
P 		2784.2
(-3)
P 		1589
C 3535
(1.4)
P 		2999.9
(-0.9)
P 		3059.3
(-0.6)
P 		3370.9
(0.7)
P 		3906.2
(3)
P 		2500.7
(-3)
P 		1406
avr 3259.6
(-0.4) 		3124.8
(-1.1) 		3228.1
(-0.6) 		3392.7
(0.2) 		3939.8
(3) 		2748.9
(-3) 		1191
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1296
1 2 3 4 range
A 2144.5
(3)
P 		1474.9
(-0.1)
P 		1742.1
(1.1)
P 		848.2
(-3)
P 		1296
B 1959.9
(3)
P 		1550.2
(-0.2)
P 		1635.1
(0.4)
P 		1198.6
(-3)
P 		761
C 1989.2
(2.7)
P 		1908.2
(2.2)
P 		2046.7
(3)
P 		1028.4
(-3)
P 		1018
avr 2031.2
(3) 		1644.4
(0.7) 		1808
(1.7) 		1025.1
(-3) 		1006

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1206
1 2 3 4 5 6 range
A 571.1
(-3)
P 		1143.3
(3)
P 		649.9
(-2.2)
P 		872.4
(0.2)
P 		1068.2
(2.2)
P 		721.1
(-1.4)
P 		572
B 1094.5
(-1.6)
P 		1051.7
(-1.9)
P 		884.1
(-3)
P 		891.9
(-2.9)
P 		1776.7
(3)
P 		1038.2
(-2)
P 		893
C 1310.3
(3)
P 		915.3
(-0.7)
P 		778.6
(-2)
P 		677.9
(-3)
P 		1264
(2.6)
P 		837.1
(-1.5)
P 		632
avr 992
(-0.8) 		1036.8
(-0.3) 		770.9
(-3) 		814.1
(-2.6) 		1369.6
(3) 		865.5
(-2.1) 		599
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176145_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10549
Sequence Type Exemplar sequence
Representative Public ID K08D12.3 NCBI
Target Description K08D12.3 /REP_DB=WormBase Gene ID /WP=CE21045 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171273(11), NM_171274(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171273 NCBI Caenorhabditis elegans K08D12.3a (K08D12.3) mRNA, complete cds. 11/11 A 
NM_171274 NCBI Caenorhabditis elegans K08D12.3b (K08D12.3) mRNA, complete cds. 11/11 None
SNAP00000039171 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1710265:1710919:1 11/11 None
GENEFINDER00000039180 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1710265:1710919:1 11/11 None
K08D12.3a.1 ENSEMBL cdna:known chromosome:CEL140:IV:1710256:1711126:1 gene:K08D12.3 11/11 None
K08D12.3a.2 ENSEMBL cdna:known chromosome:CEL140:IV:1710263:1711126:1 gene:K08D12.3 11/11 None
K08D12.3b ENSEMBL cdna:known chromosome:CEL140:IV:1710265:1710800:1 gene:K08D12.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1710270-1710925(+) 100.0 100.0
Public Domain and Genome References
Ensembl K08D12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84542 Hypothetical protein K08D12.3a [Caenorhabditis elegans] ref|NP_741323.1| K08D12.3a [Caenorhabditis elegans] 2.0E-52
blast CAE67981 Hypothetical protein CBG13587 [Caenorhabditis briggsae] 1.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR001878 EMBL-EBI Zn-finger, CCHC type 2.0E-8
Pfam IPR001878 EMBL-EBI Zn-finger, CCHC type 4.8E-9
Pfam IPR001878 EMBL-EBI Zn-finger, CCHC type 5.5E-8
Pfam IPR001878 EMBL-EBI Zn-finger, CCHC type 1.4E-8
Pfam IPR001878 EMBL-EBI Zn-finger, CCHC type 8.7E-8
Sequence
Target Sequence 
>C. ELEGANS:176145_AT
atgtcggacagaaattgctacaagtgccaacagccgggacatatctcccgcaactgcccg
aatggcgagtccgatggtggacgtagaggaggtggaggaggatccacctgctacaactgc
caagagactggacacttctctcgcgattgccccaagggaggatccggagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCGGACAGAAATTGCTACAAGT 266 49 13 Antisense
TGCTACAAGTGCCAACAGCCGGGAC 103 581 28 Antisense
AACAGCCGGGACATATCTCCCGCAA 297 135 41 Antisense
CGCAACTGCCCGAATGGCGAGTCCG 415 251 61 Antisense
CCCGAATGGCGAGTCCGATGGTGGA 299 261 69 Antisense
GATGGTGGACGTAGAGGAGGTGGAG 665 407 85 Antisense
GTGGAGGAGGATCCACCTGCTACAA 95 491 104 Antisense
CACCTGCTACAACTGCCAAGAGACT 481 201 117 Antisense
CAAGAGACTGGACACTTCTCTCGCG 422 185 133 Antisense
TCTCGCGATTGCCCCAAGGGAGGAT 496 607 151 Antisense
ATTGCCCCAAGGGAGGATCCGGAGG 466 3 158 Antisense
Sequence Source Affymetrix Proprietary Database