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[176134_at]

Description Y119D3B.J /REP_DB=WormBase Gene ID /WP=CE26486 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 84      4grp_total > 10      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 3.7
(-3)
A 		15.3
(-1.8)
A 		10.8
(-2.3)
A 		61.4
(3)
A 		58
B 20.4
(3)
A 		8
(-2.9)
A 		7.7
(-3)
A 		19.2
(2.4)
A 		13
C 7.4
(-2.6)
A 		10.5
(-2.2)
A 		3.2
(-3)
A 		59.9
(3)
A 		57
avr 10.5
(-2.5) 		11.3
(-2.4) 		7.2
(-3) 		46.8
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 4.8
(-3)
A 		11
(0.6)
A 		6.5
(-2)
A 		15
(3)
A 		12.8
(1.7)
A 		12.3
(1.4)
A 		10
B 5
(-2.4)
A 		8
(-1.1)
A 		8.2
(-1.1)
A 		17.6
(3)
A 		4.1
(-2.8)
A 		3.7
(-3)
A 		14
C 3
(-3)
A 		3.6
(-2.8)
A 		7.1
(-1.4)
A 		7.7
(-1.2)
A 		18.4
(3)
A 		10.7
(0)
A 		15
avr 4.3
(-3) 		7.5
(-0.9) 		7.3
(-1) 		13.4
(3) 		11.8
(1.9) 		8.9
(0) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.4
(-3)
A 		3.3
(-1.5)
A 		2.8
(-1.9)
A 		9
(3)
A 		8
B 6.5
(3)
A 		2
(-2.7)
A 		1.8
(-3)
A 		4.1
(-0.1)
A 		5
C 2.5
(-2.1)
A 		3
(-1.7)
A 		1.1
(-3)
A 		10.1
(3)
A 		9
avr 3.5
(-1.3) 		2.8
(-2.1) 		1.9
(-3) 		7.7
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 0.9
(-3)
A 		3.9
(3)
A 		1.4
(-2)
A 		3.8
(2.8)
A 		3.9
(3)
A 		3
(1.2)
A 		3
B 1.8
(-2.3)
A 		2.7
(-0.6)
A 		2
(-1.9)
A 		4.6
(3)
A 		1.7
(-2.4)
A 		1.4
(-3)
A 		3
C 1.1
(-3)
A 		1.1
(-3)
A 		1.8
(-2.1)
A 		1.5
(-2.5)
A 		6
(3)
A 		3.6
(0.1)
A 		5
avr 1.3
(-3) 		2.6
(0) 		1.7
(-2.1) 		3.3
(1.6) 		3.9
(3) 		2.7
(0.2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176134_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19073
Sequence Type Exemplar sequence
Representative Public ID Y119D3B.J NCBI
Target Description Y119D3B.J /REP_DB=WormBase Gene ID /WP=CE26486 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064944
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000002030 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1185007:1193395:-1 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1184593-1191287(-) 82.35 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29716 Hypothetical protein Y119D3B.14 [Caenorhabditis elegans] ref|NP_497345.1| Y119D3B.14 [Caenorhabditis elegans] 0.0
blast CAE69118 Hypothetical protein CBG15144 [Caenorhabditis briggsae] 0.0
blast XP_535276 PREDICTED: similar to mitochondrial elongation factor G2 isoform 1 [Canis familiaris] 1.0E-114
Protein Domains
Database ID Description E-Value
scop a.4.5.SCF ubiquitin ligase complex WHB domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; SCF ubiquitin ligase complex WHB domain 7.90000009536743
Pfam IPR000640 EMBL-EBI Elongation factor G, C-terminal 2.8E-5
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 1.1E-43
Pfam IPR005517 EMBL-EBI Elongation factor G, domain IV 0.0020
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 7.0E-5
Sequence
Target Sequence 
>C. ELEGANS:176134_AT
aagagccaccggaattgttttcacaataaaattttttttatcggcttcagctcaccgaga
gctttcaaatgacccccatcatgcctatgctccgacgattttgagttatggaagttccga
cacagctgaaaccggtgctacagtaacccgcgtggtgggacccaaaatatcgaaatcgtc
ggaacctagccatgatgggggtcattttgaagctgagagtgtcaggaatgataattttat
acgtttttcaagaatttccaatctttttatttttatctcacatttattctccaaacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAGCCACCGGAATTGTTTTCACA 127 153 1794 Antisense
ATTTTTTTTATCGGCTTCAGCTCAC 305 15 1824 Antisense
GGCTTCAGCTCACCGAGAGCTTTCA 53 539 1836 Antisense
GCCTATGCTCCGACGATTTTGAGTT 457 281 1876 Antisense
TATGGAAGTTCCGACACAGCTGAAA 1 655 1900 Antisense
CCGCGTGGTGGGACCCAAAATATCG 591 225 1941 Antisense
TCGAAATCGTCGGAACCTAGCCATG 48 599 1963 Antisense
GGAACCTAGCCATGATGGGGGTCAT 410 527 1974 Antisense
GGGGGTCATTTTGAAGCTGAGAGTG 183 497 1990 Antisense
TCTTTTTATTTTTATCTCACATTTA 211 615 2055 Antisense
TATCTCACATTTATTCTCCAAACGA 137 655 2067 Antisense
Sequence Source Affymetrix Proprietary Database