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[176123_at]

Description Y65B4A.C /REP_DB=WormBase Gene ID /WP=CE25530 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 89      4grp_total > 16      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 77.7
(-1.5)
A 		46.2
(-3)
A 		68.8
(-1.9)
A 		172.1
(3)
A 		126
B 24.8
(-3)
A 		42.6
(-2)
A 		137
(3)
A 		130.7
(2.7)
A 		112
C 25
(-3)
A 		92.6
(0.6)
A 		102.4
(1.1)
A 		139.1
(3)
A 		114
avr 42.5
(-3) 		60.5
(-2) 		102.7
(0.4) 		147.3
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 20.6
(-3)
A 		98.9
(0.3)
A 		60.4
(-1.3)
A 		79.5
(-0.5)
A 		90
(-0.1)
A 		164
(3)
A 		143
B 74.6
(-1.2)
A 		49.4
(-3)
A 		108.4
(1.3)
A 		60.9
(-2.2)
A 		103.6
(0.9)
A 		132.3
(3)
A 		83
C 66.2
(-1.4)
A 		67.5
(-1.3)
A 		29.8
(-3)
A 		41.8
(-2.5)
A 		162.3
(3)
A 		123.4
(1.2)
A 		133
avr 53.8
(-3) 		71.9
(-1.7) 		66.2
(-2.1) 		60.7
(-2.5) 		118.6
(1.5) 		139.9
(3) 		86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 28.3
(3)
A 		10
(-3)
A 		18
(-0.4)
A 		25.2
(2)
A 		18
B 7.9
(-3)
A 		10.5
(-2.3)
A 		31.7
(3)
A 		27.9
(2)
A 		24
C 8.3
(-3)
A 		26.6
(1)
A 		35.7
(3)
A 		23.4
(0.3)
A 		27
avr 14.8
(-3) 		15.7
(-2.6) 		28.5
(3) 		25.5
(1.7) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 3.8
(-3)
A 		35.2
(2.2)
A 		13.1
(-1.5)
A 		20.2
(-0.3)
A 		27.2
(0.9)
A 		39.9
(3)
A 		36
B 25.9
(-1.2)
A 		16.4
(-2.9)
A 		26.3
(-1.2)
A 		16.1
(-3)
A 		42.1
(1.7)
A 		49.3
(3)
A 		33
C 24.5
(-0.7)
A 		20.6
(-1.3)
A 		7.6
(-3)
A 		8.4
(-2.9)
A 		52.5
(3)
A 		41.3
(1.5)
A 		45
avr 18.1
(-2.3) 		24.1
(-1.1) 		15.7
(-2.8) 		14.9
(-3) 		40.6
(2.4) 		43.5
(3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176123_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18849
Sequence Type Exemplar sequence
Representative Public ID Y65B4A.C NCBI
Target Description Y65B4A.C /REP_DB=WormBase Gene ID /WP=CE25530 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058363(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058363 NCBI Caenorhabditis elegans Y65B4A.7 (Y65B4A.7) mRNA, complete cds. 11/11 None
SNAP00000032537 ENSEMBL cdna:SNAP chromosome:CEL140:I:645642:663552:-1 9/11 None
GENEFINDER00000032544 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:645642:647228:-1 11/11 None
Y65B4A.7 ENSEMBL cdna:known chromosome:CEL140:I:645642:647228:-1 gene:Y65B4A.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:645641-647228(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y65B4A.7
Entrez Gene 190486 Entrez gene
SwissProt Q9BL58 EMBL-EBI
WormBase CE25530 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29952 Hypothetical protein Y65B4A.8 [Caenorhabditis elegans] ref|NP_490766.2| Y65B4A.8 [Caenorhabditis elegans] 0.0
blast CAE71585 Hypothetical protein CBG18542 [Caenorhabditis briggsae] 1.0E-159
blast AAK29953 Hypothetical protein Y65B4A.7 [Caenorhabditis elegans] ref|NP_490764.1| Y65B4A.7 [Caenorhabditis elegans] 4.0E-95
blast CAE74423 Hypothetical protein CBG22156 [Caenorhabditis briggsae] 5.0E-92
blast AAH53279 Four and a half LIM domains [Danio rerio] ref|NP_954687.1| four and a half LIM domains [Danio rerio] 2.0E-11
blast BAE06429.1 Ci-Fhl1/2/3 [Ciona intestinalis] 1.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 9.1E-5
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 2.5E-7
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 2.5E-7
Pfam IPR004820 EMBL-EBI Cytidylyltransferase 2.7E-8
Pfam IPR001977 EMBL-EBI Dephospho-CoA kinase 4.0E-39
Sequence
Target Sequence 
>C. ELEGANS:176123_AT
gcaaaggctgcacaacaacgctgactcgtcaagaagctcaaactggaaaagtgatcaaaa
ttcaagacgagatgtatcatatttcatgcctgaaatgctcggtttgtcagacagtcattg
gcatgactccgtgttatccaatgcccgcgccgtacggtaacgcgctccgttgcgcggact
gtcaccgggcggccaagtcaccaaaatgccacgcgtgcaaattgccgacttttgagagat
gcgtgtcggcgttcaatgtccactggcatatggcatgtttcaagtgtaaagcgtgtcgcc
acccgattaaaggtcaagagtacgtggttcaccagggatgtgcctacgatgaggattgct
atcaccataccattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAAGGCTGCACAACAACGCTGAC 155 319 74 Antisense
GAAATGCTCGGTTTGTCAGACAGTC 476 361 165 Antisense
TCATTGGCATGACTCCGTGTTATCC 320 619 188 Antisense
GGCGGCCAAGTCACCAAAATGCCAC 277 539 261 Antisense
AAAATGCCACGCGTGCAAATTGCCG 700 131 276 Antisense
TGAGAGATGCGTGTCGGCGTTCAAT 82 565 306 Antisense
GGCGTTCAATGTCCACTGGCATATG 1 543 321 Antisense
CAAGTGTAAAGCGTGTCGCCACCCG 556 183 354 Antisense
GTGTCGCCACCCGATTAAAGGTCAA 523 487 366 Antisense
CGTGGTTCACCAGGGATGTGCCTAC 14 245 396 Antisense
GAGGATTGCTATCACCATACCATTC 571 403 424 Antisense
Sequence Source Affymetrix Proprietary Database