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[176112_at]

Description Y65B4A.I /REP_DB=WormBase Gene ID /WP=CE22727 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 33      4grp_highest_8_8 > 63      4grp_high_8_8 > 28      6grp_highest_nonorm > 80      6grp_total > 02      4grp_total > 36      6grp_highest_sub > De      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 285.1
(3)
P 		276.3
(2.5)
P 		177.2
(-3)
P 		237.8
(0.4)
P 		108
B 214.4
(-0.5)
P 		295.7
(3)
P 		156.7
(-3)
P 		271.6
(2)
P 		139
C 223.4
(-0.4)
P 		284.3
(3)
P 		177.4
(-3)
P 		281.1
(2.8)
P 		107
avr 241
(0.7) 		285.4
(3) 		170.4
(-3) 		263.5
(1.9) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 214.2
(2)
P 		165.3
(-3)
P 		181.2
(-1.4)
P 		189.9
(-0.5)
P 		209.5
(1.5)
P 		223.6
(3)
P 		58
B 245.4
(2.1)
P 		259.7
(3)
P 		186.6
(-1.8)
P 		169.3
(-3)
P 		232.8
(1.2)
P 		168.6
(-3)
P 		91
C 186.7
(-1.3)
P 		276
(3)
P 		274.3
(2.9)
P 		270.3
(2.7)
P 		218.5
(0.2)
P 		152.1
(-3)
P 		124
avr 215.4
(0.9) 		233.7
(3) 		214
(0.7) 		209.8
(0.3) 		220.3
(1.5) 		181.4
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 103.8
(3)
P 		60
(-0.8)
P 		46.3
(-2)
P 		34.8
(-3)
P 		69
B 68.4
(2.2)
P 		73
(3)
P 		36.3
(-3)
P 		57.9
(0.5)
P 		37
C 73.9
(1.6)
P 		81.7
(3)
P 		61.8
(-0.5)
P 		47.4
(-3)
P 		34
avr 82
(3) 		71.6
(1.2) 		48.1
(-2.8) 		46.7
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 39.5
(-3)
P 		58.8
(1.9)
P 		39.5
(-3)
P 		48.2
(-0.8)
P 		63.2
(3)
P 		54.4
(0.8)
P 		24
B 85.4
(1.9)
P 		86.5
(2)
P 		45.3
(-2.9)
P 		44.8
(-3)
P 		94.6
(3)
P 		62.9
(-0.8)
P 		50
C 69.2
(0.3)
P 		84.2
(3)
P 		69.8
(0.4)
P 		54.4
(-2.4)
P 		70.7
(0.6)
P 		50.9
(-3)
P 		33
avr 64.7
(0.4) 		76.5
(3) 		51.5
(-2.5) 		49.1
(-3) 		76.2
(2.9) 		56.1
(-1.5) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176112_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18853
Sequence Type Exemplar sequence
Representative Public ID Y65B4A.I NCBI
Target Description Y65B4A.I /REP_DB=WormBase Gene ID /WP=CE22727 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058362(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058362 NCBI Caenorhabditis elegans Y65B4A.2 (Y65B4A.2) mRNA, complete cds. 10/11 None
SNAP00000032536 ENSEMBL cdna:SNAP chromosome:CEL140:I:645456:653748:1 10/11 None
GENEFINDER00000032548 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:647810:653748:1 10/11 None
Y65B4A.2 ENSEMBL cdna:known chromosome:CEL140:I:645454:653978:1 gene:Y65B4A.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:648444-649756(+) 77.61 90.94
Public Domain and Genome References
Gene Title
Ensembl Y65B4A.2
Entrez Gene 171655 Entrez gene
SwissProt Q9BL59 EMBL-EBI
WormBase CE27300 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8234 cysteine-type peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29958 Hypothetical protein Y65B4A.2 [Caenorhabditis elegans] ref|NP_490763.1| Y65B4A.2 [Caenorhabditis elegans] 0.0
blast CAE61308 Hypothetical protein CBG05143 [Caenorhabditis briggsae] 0.0
blast AAA92327 Cysteine protease related protein 4 [Caenorhabditis elegans] ref|NP_504682.1| Cysteine PRotease related family member (cpr-4) [Caenorhabditis elegans] sp|P43508|CPR4_CAEEL Cathepsin B-like cysteine proteinase 4 precursor (Cysteine protease-related 4) gb|AAA98783.1| cathepsin B-like cysteine proteinase gb|AAA98785.1| cathepsin B-like cysteine proteinase [Caenorhabditis elegans] 3.0E-39
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490763.1 1 21-43
Y65B4A.2 1 21-43
SNAP00000032536 1 21-43
Sequence
Target Sequence 
>C. ELEGANS:176112_AT
tctactccggccgtggccgagtcaaaatggcttaattcggcaaattcttacatttcaaaa
tatgagggaagccagaaacgtgtgtcacattgaacttgagaactttaacagctccgatgt
tccgaaaaacttcgacgctcgtcagaaatggccgaattgcccgtcaatctcgaatgttcc
aaatcagggaggatgcgggagttgcttcgcggtcgccgccgccggtgtcgcctccgaccg
tgcctgtattcactcgaatggaacttttaagtcacttttgtccgaggaggatattattgg
atgctgctcggtgtgcggaaattgttacggcggagatccactgaaggccttgacctattg
ggttaatcagggacttgttacaggtggccgtgacggttgccgtccctactcgtttgacct
gtcgtgtggagtcccgtgctcgccggccacgttctttgaggccgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTACTCCGGCCGTGGCCGAGTCAA 513 613 54 Antisense
AACTTTAACAGCTCCGATGTTCCGA 681 137 154 Antisense
TTCGACGCTCGTCAGAAATGGCCGA 316 695 184 Antisense
CCGAATTGCCCGTCAATCTCGAATG 668 261 205 Antisense
GGAGGATGCGGGAGTTGCTTCGCGG 255 515 241 Antisense
CCGTGCCTGTATTCACTCGAATGGA 511 265 291 Antisense
ATTATTGGATGCTGCTCGGTGTGCG 664 3 346 Antisense
GATCCACTGAAGGCCTTGACCTATT 629 415 388 Antisense
TACAGGTGGCCGTGACGGTTGCCGT 201 643 432 Antisense
CTACTCGTTTGACCTGTCGTGTGGA 635 223 459 Antisense
GCCGGCCACGTTCTTTGAGGCCGAG 469 1 495 Antisense
Sequence Source Affymetrix Proprietary Database