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[176109_at]

Description E01A2.2 /REP_DB=WormBase Gene ID /WP=CE20643 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 59      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 74      6grp_total > 39      4grp_total > 60      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 666
1 2 3 4 range
A 649
(0.4)
P 		706.6
(1.2)
P 		841.5
(3)
P 		399.7
(-3)
P 		442
B 829.4
(3)
P 		551
(0.1)
P 		687.1
(1.5)
P 		258.9
(-3)
P 		571
C 649.5
(1)
P 		754.7
(1.9)
P 		875.2
(3)
P 		209.2
(-3)
P 		666
avr 709.3
(1.9) 		670.8
(1.5) 		801.3
(3) 		289.3
(-3) 		512

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 463
1 2 3 4 5 6 range
A 611.3
(0.5)
P 		780.4
(3)
P 		376.3
(-3)
P 		656.6
(1.2)
P 		741.2
(2.4)
P 		774.5
(2.9)
P 		404
B 641.7
(0.1)
P 		819.2
(3)
P 		446.3
(-3)
P 		627.8
(-0.1)
P 		642.8
(0.2)
P 		684.8
(0.8)
P 		373
C 839.4
(3)
P 		589.6
(-0.6)
P 		718.5
(1.3)
P 		418.8
(-3)
P 		747.6
(1.7)
P 		525.9
(-1.5)
P 		421
avr 697.5
(2.1) 		729.7
(3) 		513.7
(-3) 		567.7
(-1.5) 		710.5
(2.5) 		661.7
(1.1) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 270
1 2 3 4 range
A 236.3
(3)
P 		153.4
(0.2)
P 		220
(2.4)
P 		58.5
(-3)
P 		178
B 264.5
(3)
P 		136.1
(-0.7)
P 		159.2
(-0)
P 		55.2
(-3)
P 		209
C 215
(1)
P 		217
(1)
P 		305
(3)
P 		35.3
(-3)
P 		270
avr 238.6
(3) 		168.8
(0.8) 		228.1
(2.7) 		49.7
(-3) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 229
1 2 3 4 5 6 range
A 112.7
(-2.1)
P 		277.6
(3)
P 		81.9
(-3)
P 		166.5
(-0.4)
P 		223.7
(1.3)
P 		188.6
(0.3)
P 		196
B 223.3
(1.2)
P 		272.7
(3)
P 		108.4
(-3)
P 		166.3
(-0.9)
P 		261.1
(2.6)
P 		255.4
(2.4)
P 		164
C 311.1
(3)
P 		179.9
(-0.5)
P 		182.9
(-0.4)
P 		84.2
(-3)
P 		241.9
(1.2)
P 		176
(-0.6)
P 		227
avr 215.7
(1.6) 		243.4
(3) 		124.4
(-3) 		139
(-2.3) 		242.2
(2.9) 		206.7
(1.1) 		119
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176109_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1549
Sequence Type Exemplar sequence
Representative Public ID E01A2.2 NCBI
Target Description E01A2.2 /REP_DB=WormBase Gene ID /WP=CE20643 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058901(11), NM_182055(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058901 NCBI Caenorhabditis elegans E01A2.2a (E01A2.2) mRNA, complete cds. 11/11 A 
NM_182055 NCBI Caenorhabditis elegans E01A2.2b (E01A2.2) mRNA, complete cds. 11/11 A 
SNAP00000008534 ENSEMBL cdna:SNAP chromosome:CEL140:I:4132502:4135063:1 11/11 None
GENEFINDER00000008592 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4132502:4135063:1 11/11 None
E01A2.2a ENSEMBL cdna:known chromosome:CEL140:I:4132501:4135240:1 gene:E01A2.2 11/11 None
E01A2.2b ENSEMBL cdna:known chromosome:CEL140:I:4133159:4135063:1 gene:E01A2.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4132501-4135063(+) 100.0 100.0
Public Domain and Genome References
Ensembl E01A2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK85459 Hypothetical protein E01A2.2a [Caenorhabditis elegans] ref|NP_491302.1| E01A2.2a [Caenorhabditis elegans] sp|Q966L5|ARS2_CAEEL Arsenite-resistance protein 2 homolog 0.0
blast CAE66718 Hypothetical protein CBG12064 [Caenorhabditis briggsae] 0.0
blast AAO38662 Hypothetical protein E01A2.2b [Caenorhabditis elegans] ref|NP_871855.1| E01A2.2b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007042 EMBL-EBI Arsenite-resistance protein 2 1.8E-57
Pfam IPR007042 EMBL-EBI Arsenite-resistance protein 2 1.8E-57
Sequence
Target Sequence 
>C. ELEGANS:176109_AT
gtatcaatcactactgacgaggatggagctctcgtcgttccacagaaatttgtgaacgat
tttatttcgggattcaatagccgcattgagaaagggttgattgaaaagcagtatgtaagc
gaggaagaattggagaaaatgggcaagaaggatggagaaaaggaagttgaagcttttatt
caaaagaatactgtcgagttggcaaaggataaatggctctgtccgctttctggaaagaaa
ttcaagggacctgaattcatccgaaagcacttgcagtctaaacatgaagacaagctggag
gaagcgagagcagaagctgactttttcaacaattatcttgccgacgcccaaagaccagtc
gactgtgagcccaaacaagctccaagagatgatcatcgtggaggtggtggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATCAATCACTACTGACGAGGATG 20 453 1522 Antisense
CTCTCGTCGTTCCACAGAAATTTGT 543 231 1550 Antisense
TATTTCGGGATTCAATAGCCGCATT 488 655 1584 Antisense
AAAGGATAAATGGCTCTGTCCGCTT 344 121 1725 Antisense
GGGACCTGAATTCATCCGAAAGCAC 428 495 1767 Antisense
GAAAGCACTTGCAGTCTAAACATGA 621 359 1784 Antisense
GAGCAGAAGCTGACTTTTTCAACAA 613 389 1829 Antisense
TTCAACAATTATCTTGCCGACGCCC 503 685 1846 Antisense
CGACGCCCAAAGACCAGTCGACTGT 619 249 1863 Antisense
CAGTCGACTGTGAGCCCAAACAAGC 469 205 1877 Antisense
AGATGATCATCGTGGAGGTGGTGGT 487 65 1908 Antisense
Sequence Source Affymetrix Proprietary Database