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[176107_at]

Description Y50D4A.F /REP_DB=WormBase Gene ID /WP=CE25345 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 95      6grp_total > 98      4grp_total > 02      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3394
1 2 3 4 range
A 530.1
(-3)
P
637.4
(-2.8)
P
630.8
(-2.8)
P
3906.1
(3)
P
3376
B 628.6
(-2.8)
P
511.7
(-3)
P
915.2
(-2.2)
P
3534
(3)
P
3022
C 548
(-3)
P
608.3
(-2.8)
P
681
(-2.6)
P
2497.4
(3)
P
1949
avr 568.9
(-3)
585.8
(-3)
742.3
(-2.6)
3312.5
(3)
2744

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 582
1 2 3 4 5 6 range
A 442.8
(-2.5)
P
537.2
(-1.4)
P
400
(-3)
P
608.9
(-0.5)
P
911.1
(3)
P
856.9
(2.4)
P
511
B 502.4
(-1.9)
P
501.1
(-1.9)
P
395
(-3)
P
656.2
(-0.3)
P
847
(1.7)
P
974.6
(3)
P
580
C 509
(-2.4)
P
453.5
(-3)
P
526.7
(-2.2)
P
505.1
(-2.4)
P
822.8
(1.2)
P
977.4
(3)
P
524
avr 484.7
(-2.5)
497.3
(-2.3)
440.6
(-3)
590.1
(-1.2)
860.3
(2.1)
936.3
(3)
496
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 627
1 2 3 4 range
A 193
(-2.2)
P
138.4
(-3)
P
164.9
(-2.6)
P
571.8
(3)
P
433
B 200.4
(-2.3)
P
126.4
(-3)
P
212.1
(-2.2)
P
753.1
(3)
P
627
C 181.4
(-2.8)
P
174.9
(-3)
P
237.4
(-1.5)
P
421.1
(3)
P
246
avr 191.6
(-2.4)
146.6
(-3)
204.8
(-2.2)
582
(3)
435

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 282
1 2 3 4 5 6 range
A 81.6
(-3)
P
191
(0.4)
P
87.1
(-2.8)
P
154.4
(-0.7)
P
274.9
(3)
P
208.6
(0.9)
P
193
B 174.8
(-1.2)
P
166.8
(-1.4)
P
95.9
(-3)
P
173.8
(-1.3)
P
344.1
(2.6)
P
363.4
(3)
P
268
C 188.7
(-0.7)
P
138.4
(-2)
P
134.1
(-2.1)
P
101.6
(-3)
P
266.2
(1.4)
P
327.2
(3)
P
226
avr 148.4
(-1.7)
165.4
(-1.2)
105.7
(-3)
143.3
(-1.8)
295.1
(2.9)
299.7
(3)
194
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176107_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19504
Sequence Type Exemplar sequence
Representative Public ID Y50D4A.F NCBI
Target Description Y50D4A.F /REP_DB=WormBase Gene ID /WP=CE25345 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070992(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_070992 NCBI Caenorhabditis elegans Y50D4A.2 (Y50D4A.2) mRNA, complete cds. 11/11 None
GENEFINDER00000016440 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1196148:1196825:1 11/11 None
Y50D4A.2.2 ENSEMBL cdna:known chromosome:CEL140:V:1196138:1196829:1 gene:Y50D4A.2 11/11 None
Y50D4A.2.1 ENSEMBL cdna:known chromosome:CEL140:V:1196146:1196881:1 gene:Y50D4A.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016426 8/11 Cross Hyb Matching Probes None
Y50D4A.5 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1196111-1196829(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4A.2
Entrez Gene 178617 Entrez gene
SwissProt Q8WTM8 EMBL-EBI
WormBase CE30026 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68503 Hypothetical protein Y50D4A.2 [Caenorhabditis elegans] ref|NP_503393.2| Y50D4A.2 [Caenorhabditis elegans] 7.0E-74
blast CAE58015 Hypothetical protein CBG01085 [Caenorhabditis briggsae] 6.0E-56
blast AAK68503 Hypothetical protein Y50D4A.2 [Caenorhabditis elegans] ref|NP_503393.2| Y50D4A.2 [Caenorhabditis elegans] 4.0E-55
blast CAE58015 Hypothetical protein CBG01085 [Caenorhabditis briggsae] 1.0E-44
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503393.2 3 10-32,122-144,159-181
Y50D4A.2.2 3 10-32,122-144,159-181
Y50D4A.2.1 3 10-32,122-144,159-181
GENEFINDER00000016440 1 9-31
Sequence
Target Sequence
>C. ELEGANS:176107_AT
tcatcgctgtaatctgttctgcaacatttgcgatttatagctcacaaattgtttccacca
tttctagcacgatcaaatcactgtccaagccaaagcccagcccaaaagtcgtcgcgttga
agctaaaaattgccgatttgaagcgagagctgcacggaatctctccgaccgccgaatttg
cacgatatttcaagaaggatcgagaaatgaataaagccagcgaagaattagctgctttgg
aagccgctagcccgtctgaaaactcgaggaacttgaaaatcgacacagttgtcaaagttt
ttatgcaattctccgcactttttcttctgagacacgtgtccgccatcactgcctactgca
ttccggatactattttttggcctttcaacgtacttgttcgattcccagcggttttcggaa
atgactcctgcccggccgaattcgccgaagtttccggattcgcgctcacatttctgatga
tccatctgctaaatctggcgctc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
TCATCGCTGTAATCTGTTCTGCAAC 128 621 89 Antisense
TTTCCACCATTTCTAGCACGATCAA 594 669 140 Antisense
CACGATCAAATCACTGTCCAAGCCA 451 199 156 Antisense
GACCGCCGAATTTGCACGATATTTC 48 383 255 Antisense
GCTAGCCCGTCTGAAAACTCGAGGA 76 301 334 Antisense
TCTCCGCACTTTTTCTTCTGAGACA 417 611 398 Antisense
TCAACGTACTTGTTCGATTCCCAGC 525 613 473 Antisense
GGTTTTCGGAAATGACTCCTGCCCG 51 505 498 Antisense
ATTCGCCGAAGTTTCCGGATTCGCG 389 7 528 Antisense
TTCGCGCTCACATTTCTGATGATCC 615 697 547 Antisense
GATCCATCTGCTAAATCTGGCGCTC 16 417 567 Antisense
Sequence Source Affymetrix Proprietary Database