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[176103_at]

Description Y50D4A.H /REP_DB=WormBase Gene ID /WP=CE25347 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 86      4grp_total > 04      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 69.5
(-0.5)
A 		9.1
(-3)
A 		31.8
(-2)
A 		151.7
(3)
P 		143
B 59.1
(1.1)
A 		65.1
(1.9)
A 		29.3
(-3)
A 		73.2
(3)
P 		44
C 55.1
(-1.7)
A 		30.8
(-3)
A 		31.5
(-3)
A 		145.3
(3)
P 		115
avr 61.2
(-1) 		35
(-2.7) 		30.9
(-3) 		123.4
(3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 4.3
(-3)
A 		66.3
(2.1)
A 		51.4
(0.9)
A 		38.9
(-0.2)
A 		30
(-0.9)
A 		77.5
(3)
A 		73
B 25.4
(-1.9)
A 		14.9
(-3)
A 		38.3
(-0.4)
A 		18.4
(-2.6)
A 		69.8
(3)
A 		64
(2.4)
P 		55
C 24.4
(-1.2)
A 		13
(-3)
A 		13.2
(-3)
A 		42.9
(1.6)
A 		39.5
(1.1)
A 		51.8
(3)
A 		39
avr 18
(-3) 		31.4
(-1.3) 		34.3
(-0.9) 		33.4
(-1) 		46.4
(0.7) 		64.4
(3) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 25.3
(3)
A 		2
(-3)
A 		8.3
(-1.4)
A 		22.2
(2.2)
P 		23
B 18.8
(3)
A 		16.1
(1.7)
A 		6.8
(-3)
A 		15.6
(1.4)
P 		12
C 18.2
(0.6)
A 		8.9
(-3)
A 		11
(-2.2)
A 		24.5
(3)
P 		16
avr 20.8
(3) 		9
(-2.9) 		8.7
(-3) 		20.8
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 0.8
(-3)
A 		23.6
(3)
A 		11.2
(-0.3)
A 		9.9
(-0.6)
A 		9.1
(-0.8)
A 		18.9
(1.8)
A 		23
B 8.8
(-2)
A 		5
(-3)
A 		9.3
(-1.9)
A 		4.9
(-3)
A 		28.4
(3)
A 		23.8
(1.8)
P 		24
C 9.1
(-0.5)
A 		4
(-2.7)
A 		3.4
(-3)
A 		8.6
(-0.8)
A 		12.8
(1.1)
A 		17.3
(3)
A 		14
avr 6.2
(-3) 		10.9
(-1) 		8
(-2.2) 		7.8
(-2.3) 		16.8
(1.6) 		20
(3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176103_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19507
Sequence Type Exemplar sequence
Representative Public ID Y50D4A.H NCBI
Target Description Y50D4A.H /REP_DB=WormBase Gene ID /WP=CE25347 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029081, NM_070993
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000016439 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1207139:1207517:1 None
Y50D4A.5 ENSEMBL cdna:known chromosome:CEL140:V:1207139:1207623:1 gene:Y50D4A.5 None
NM_070993 NCBI Caenorhabditis elegans Y50D4A.1 (Y50D4A.1) mRNA, complete cds. A 
Y50D4A.1 ENSEMBL cdna:known chromosome:CEL140:V:1207719:1219676:1 gene:Y50D4A.1 A 
SNAP00000016433 ENSEMBL cdna:SNAP chromosome:CEL140:V:1207139:1219619:1 None
NM_001029081 NCBI Caenorhabditis elegans Y50D4A.5 (Y50D4A.5) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020820 1/11 Cross Hyb Matching Probes None
GENEFINDER00000020836 1/11 Cross Hyb Matching Probes None
SNAP00000004112 1/11 Cross Hyb Matching Probes None
SNAP00000016432 5/11 Cross Hyb Matching Probes None
SNAP00000038923 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1205165-1207988(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl Y50D4A.5
Y50D4A.1
Entrez Gene 178618 Entrez gene
259612 Entrez gene
SwissProt Q95Y57 EMBL-EBI
Q7KX42 EMBL-EBI
WormBase CE27637 Wormbase
CE36961 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68502 Hypothetical protein Y50D4A.1 [Caenorhabditis elegans] ref|NP_503394.1| Y50D4A.1 [Caenorhabditis elegans] 0.0
blast CAE66580 Hypothetical protein CBG11897 [Caenorhabditis briggsae] 1.0E-128
blast CAE66580 Hypothetical protein CBG11897 [Caenorhabditis briggsae] 1.0E-124
blast CAE58014 Hypothetical protein CBG01084 [Caenorhabditis briggsae] 1.0E-103
blast CAE58014 Hypothetical protein CBG01084 [Caenorhabditis briggsae] 2.0E-98
blast AAQ23125 Hypothetical protein Y50D4A.5 [Caenorhabditis elegans] ref|NP_001024252.1| Y50D4A.5 [Caenorhabditis elegans] 1.0E-26
Sequence
Target Sequence 
>C. ELEGANS:176103_AT
gtgccaagtgcggagacacgtctggagaagtcaaacacttattcacctgcttccccgcag
taccaatgtcaccgatggacctgtggaagaagcctaaagctgtggcagcggccctcgggc
tctccacaaccctcgtcgaccctggaggaaactctacctcccccatgtccgatgaggatg
actacaattcagacgaaaatgtggaggatcttgacgtgaaaatcgattatatcggtgaaa
gtattgaaattgagccggagaacccgtacttcttagcgatttttgacgattttatgatct
ccaccgactcagaagcacgtttccagaggcgagaaaagtctcaaatggaaaaacctgcct
ctaaatcgaagcgaaaacctaaagagaccagtgatgatgtagacgcagacgttttgccag
aaaaaggccttcacaattcggatggttttctattttatcacgcgtcgttccggccgttaa
cctcagctcagcataggcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCAAGTGCGGAGACACGTCTGG 421 477 17 Antisense
AGTCAAACACTTATTCACCTGCTTC 629 61 45 Antisense
CCCGCAGTACCAATGTCACCGATGG 99 261 70 Antisense
AGCCTAAAGCTGTGGCAGCGGCCCT 671 81 107 Antisense
GTCGACCCTGGAGGAAACTCTACCT 66 473 151 Antisense
TGACGATTTTATGATCTCCACCGAC 385 571 300 Antisense
TCAGAAGCACGTTTCCAGAGGCGAG 636 623 325 Antisense
AAAACCTGCCTCTAAATCGAAGCGA 594 131 366 Antisense
AAGGCCTTCACAATTCGGATGGTTT 14 159 440 Antisense
TTCTATTTTATCACGCGTCGTTCCG 255 691 464 Antisense
GTTAACCTCAGCTCAGCATAGGCGA 434 445 492 Antisense
Sequence Source Affymetrix Proprietary Database