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[176101_at]

Description T25D3.4 /REP_DB=WormBase Gene ID /WP=CE19601 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_highest_8_8 > 03      4grp_high_8_8 > 37      6grp_total > 35      4grp_total > 42      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 66.8
(-1.1)
P 		70.9
(-0.2)
P 		85.5
(3)
P 		58
(-3)
P 		28
B 84
(3)
P 		62.8
(-0.8)
P 		84
(3)
P 		50.4
(-3)
P 		34
C 50.4
(-3)
P 		110
(3)
P 		57.6
(-2.3)
P 		103.3
(2.3)
P 		60
avr 67.1
(-3) 		81.2
(3) 		75.7
(0.7) 		70.6
(-1.5) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 28.4
(-3)
A 		43.6
(-1.3)
P 		37.3
(-2)
A 		56.3
(0.1)
A 		47.1
(-0.9)
A 		82.4
(3)
P 		54
B 63.1
(3)
P 		52.1
(0.4)
P 		54.4
(1)
P 		45.9
(-1)
A 		39.6
(-2.5)
A 		37.5
(-3)
P 		26
C 72.9
(1.7)
P 		85.1
(3)
P 		71.5
(1.6)
P 		41.1
(-1.6)
A 		49.6
(-0.7)
A 		27.6
(-3)
A 		58
avr 54.8
(0.8) 		60.3
(3) 		54.4
(0.6) 		47.8
(-2) 		45.4
(-3) 		49.2
(-1.5) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 24.3
(3)
P 		15.4
(-0.4)
P 		22.4
(2.3)
P 		8.5
(-3)
P 		16
B 26.8
(3)
P 		15.5
(-1.2)
P 		19.5
(0.3)
P 		10.7
(-3)
P 		16
C 16.7
(-3)
P 		31.6
(3)
P 		20.1
(-1.6)
P 		17.4
(-2.7)
P 		15
avr 22.6
(3) 		20.8
(2) 		20.7
(1.9) 		12.2
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 5.2
(-3)
A 		15.5
(1.1)
P 		8.1
(-1.8)
A 		14.3
(0.7)
A 		14.2
(0.6)
A 		20.1
(3)
P 		15
B 22
(3)
P 		17.3
(0.2)
P 		13.2
(-2.3)
P 		12.1
(-3)
A 		16.1
(-0.6)
A 		14
(-1.8)
P 		10
C 27
(3)
P 		26
(2.7)
P 		18.2
(0.2)
P 		8.3
(-3)
A 		16.1
(-0.5)
A 		9.3
(-2.7)
A 		19
avr 18.1
(1.9) 		19.6
(3) 		13.2
(-1.8) 		11.6
(-3) 		15.5
(-0.1) 		14.5
(-0.8) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176101_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5146
Sequence Type Exemplar sequence
Representative Public ID T25D3.4 NCBI
Target Description T25D3.4 /REP_DB=WormBase Gene ID /WP=CE19601 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061269(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061269 NCBI Caenorhabditis elegans T25D3.4 (T25D3.4) mRNA, complete cds. 11/11 None
GENEFINDER00000025786 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:146261:151932:1 11/11 None
T25D3.4 ENSEMBL cdna:known chromosome:CEL140:II:147273:152582:1 gene:T25D3.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025781 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:147272-151932(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25D3.4
Entrez Gene 188895 Entrez gene
SwissProt Q965I9 EMBL-EBI
WormBase CE36022 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604754_AT similar to hippocampus abundant transcript 1 cfa
CANINE_2:CFA.18446.1.S1_AT similar to hippocampus abundant transcript 1 cfa
CANINE_2:CFA.19500.1.S1_S_AT similar to hippocampus abundant transcript 1 cfa
CANINE_2:CFAAFFX.30655.1.S1_AT similar to hippocampus abundant transcript 1 cfa
DROSOPHILA_2:1641635_S_AT dm
DROSGENOME1:154898_AT dm
CHICKEN:GGA.13338.1.S1_S_AT hippocampus abundant transcript 1 gga
CHICKEN:GGA.17007.1.S1_AT hippocampus abundant transcript 1 gga
CHICKEN:GGA.17007.1.A1_AT Similar to hippocampus abundant transcript 1; hippocampus abundant gene transcript 1; tetracycline transporter-like protein gga
HU35KSUBA:RC_AA428288_AT hippocampus abundant transcript 1 hs
U133_X3P:HS.21015.0.S1_3P_AT hippocampus abundant transcript 1 hs
HG-U133B:225222_AT hippocampus abundant transcript 1 hs
HG-U133_PLUS_2:225222_AT hippocampus abundant transcript 1 hs
HG-U95B:56278_AT hippocampus abundant transcript 1 hs
HG-U95E:78599_AT hippocampus abundant transcript 1 hs
HG-U133_PLUS_2:230868_AT Hippocampus abundant transcript 1 hs
HG-U133B:230868_AT Hippocampus abundant transcript 1 hs
HG-U133_PLUS_2:242374_AT Hippocampus abundant transcript 1 hs
HG-U133B:242374_AT Hippocampus abundant transcript 1 hs
HG-U95D:67366_AT Hippocampus abundant transcript 1 hs
HG-U95E:73788_AT Hippocampus abundant transcript 1 hs
HG-U95E:74681_AT Hippocampus abundant transcript 1 hs
HU35KSUBD:RC_AA609828_AT Hippocampus abundant transcript 1 hs
U133_X3P:HS.124156.0.A1_3P_AT Hippocampus abundant transcript 1 hs
U133_X3P:HS.131799.0.A1_3P_AT Hippocampus abundant transcript 1 hs
MU11KSUBA:D88315_S_AT hippocampus abundant gene transcript 1 mm
MG-U74AV2:160447_AT hippocampus abundant gene transcript 1 mm
MOE430A:1450898_AT hippocampus abundant gene transcript 1 mm
MOUSE430A_2:1450898_AT hippocampus abundant gene transcript 1 mm
MOUSE430_2:1450898_AT hippocampus abundant gene transcript 1 mm
MOE430A:1423195_AT hippocampus abundant gene transcript 1 mm
MOUSE430A_2:1423195_AT hippocampus abundant gene transcript 1 mm
MOUSE430_2:1423195_AT hippocampus abundant gene transcript 1 mm
RG-U34A:RC_AA891535_AT hippocampus abundant gene transcript 1 (predicted) rn
RAE230A:1374423_AT hippocampus abundant gene transcript 1 (predicted) rn
RAT230_2:1374423_AT hippocampus abundant gene transcript 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
15904 tetracycline transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
15520 tetracycline:hydrogen antiporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK84580 Hypothetical protein T25D3.4 [Caenorhabditis elegans] ref|NP_493670.2| T25D3.4 [Caenorhabditis elegans] 0.0
blast CAE62801 Hypothetical protein CBG06976 [Caenorhabditis briggsae] 0.0
blast T33372 hypothetical protein T25D3.1 - Caenorhabditis elegans 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493670.2 12 75-97,112-134,141-163,167-186,198-220,225-247,292-314,324-346,359-381,385-404,417-439,491-513
T25D3.4 12 75-97,112-134,141-163,167-186,198-220,225-247,292-314,324-346,359-381,385-404,417-439,491-513
GENEFINDER00000025786 12 122-144,159-181,188-210,214-233,245-267,272-294,339-361,371-393,406-428,432-451,464-486,538-560
Sequence
Target Sequence 
>C. ELEGANS:176101_AT
agggaaccgtccaaggtgtcatcaccggaattcgtggactttgcaccggtttcggtccgg
ctctcttcggcacagtgttctacttattcgacgttgatttagaagatgaaaagagcactg
gaggagcattgtcattagtggcaccgagaaagattcccgagaagattttggcgccgacga
ggaatgagagttcatggtttttccaaaataaaaacggaaagtttacggttttcaaaaatc
gtaaatattacggtttttccaagaaaacgtgcaaatttgtttcaattttcgattttccga
ttgttatcaaaaaactcgatttttcaaaaaatttgcacaatttcttaaatgaacctaata
tgcagattcaacaacaagtggaccgtgcggccgcattcgattggcaattcatacctggtc
caccattcttaataggtgcaatgatggtactattcgcattgctcatcaactcaacacttc
cacatactccaacaccgtcgaaatacttccgaaggtcttcaccatcgttatctcgacaat
cttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGGAACCGTCCAAGGTGTCATCAC 517 57 1307 Antisense
GAATTCGTGGACTTTGCACCGGTTT 446 323 1334 Antisense
GGCACAGTGTTCTACTTATTCGACG 334 535 1375 Antisense
GGAGCATTGTCATTAGTGGCACCGA 573 519 1429 Antisense
TCAACAACAAGTGGACCGTGCGGCC 86 629 1674 Antisense
TGCGGCCGCATTCGATTGGCAATTC 359 581 1692 Antisense
TTGGCAATTCATACCTGGTCCACCA 135 709 1707 Antisense
TTCGCATTGCTCATCAACTCAACAC 487 697 1759 Antisense
CTCCAACACCGTCGAAATACTTCCG 429 235 1793 Antisense
ACTTCCGAAGGTCTTCACCATCGTT 85 103 1811 Antisense
CACCATCGTTATCTCGACAATCTTC 485 201 1826 Antisense
Sequence Source Affymetrix Proprietary Database