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[176099_at]

Description Y50D4A.B /REP_DB=WormBase Gene ID /WP=CE25343 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_high1_10_10 > 49      4grp_high_8_8 > 52      6grp_total > 29      4grp_total > 44      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 102.4
(-2.4)
P 		155.4
(3)
A 		96.4
(-3)
A 		134.5
(0.9)
P 		59
B 173.2
(3)
P 		114.7
(-1.5)
P 		122.2
(-0.9)
A 		95.4
(-3)
P 		78
C 163.1
(0.6)
P 		201.2
(3)
P 		154.4
(0.1)
P 		105.5
(-3)
P 		96
avr 146.2
(1.6) 		157.1
(3) 		124.3
(-1.3) 		111.8
(-3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 141
(3)
P 		121.5
(0.4)
P 		95.5
(-3)
P 		124.2
(0.8)
P 		139.8
(2.8)
P 		100.1
(-2.4)
P 		46
B 105.7
(0.7)
P 		98.1
(-0.3)
A 		77.5
(-3)
P 		122.8
(3)
P 		99.4
(-0.1)
P 		114.7
(1.9)
P 		45
C 155
(3)
P 		96.9
(-2.1)
P 		121.3
(0)
P 		86.5
(-3)
P 		130.9
(0.9)
P 		144.9
(2.1)
P 		69
avr 133.9
(3) 		105.5
(-1.8) 		98.1
(-3) 		111.2
(-0.8) 		123.4
(1.2) 		119.9
(0.7) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 37.3
(3)
P 		33.7
(1.8)
A 		25.2
(-1.1)
A 		19.7
(-3)
P 		18
B 55.2
(3)
P 		28.3
(-1.6)
P 		28.3
(-1.6)
A 		20.3
(-3)
P 		35
C 54
(2.4)
P 		57.8
(3)
P 		53.8
(2.4)
P 		17.8
(-3)
P 		40
avr 48.8
(3) 		39.9
(1.2) 		35.8
(0.4) 		19.3
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 26
(-1.6)
P 		43.2
(3)
P 		20.8
(-3)
P 		31.5
(-0.1)
P 		42.2
(2.7)
P 		24.4
(-2)
P 		22
B 36.8
(1.5)
P 		32.7
(0.5)
A 		18.8
(-3)
P 		32.5
(0.4)
P 		40.4
(2.4)
P 		42.8
(3)
P 		24
C 57.4
(3)
P 		29.6
(-1.2)
P 		30.9
(-1)
P 		17.4
(-3)
P 		42.4
(0.8)
P 		48.5
(1.7)
P 		40
avr 40.1
(2.5) 		35.2
(0.9) 		23.5
(-3) 		27.1
(-1.8) 		41.7
(3) 		38.6
(2) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176099_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19547
Sequence Type Exemplar sequence
Representative Public ID Y50D4A.B NCBI
Target Description Y50D4A.B /REP_DB=WormBase Gene ID /WP=CE25343 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070991(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070991 NCBI Caenorhabditis elegans Y50D4A.4 (Y50D4A.4) mRNA, complete cds. 9/11 None
SNAP00000016428 ENSEMBL cdna:SNAP chromosome:CEL140:V:1184881:1195923:-1 9/11 None
GENEFINDER00000016437 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1181406:1195923:-1 9/11 None
Y50D4A.4 ENSEMBL cdna:known chromosome:CEL140:V:1184726:1196086:-1 gene:Y50D4A.4 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1194548-1195815(-) 88.7 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4A.4
Entrez Gene 178616 Entrez gene
SwissProt Q95Y58 EMBL-EBI
WormBase CE31119 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256658_AT expressed protein at
CANINE:1606141_S_AT similar to Protein C18orf8 (Colon cancer-associated protein Mic1) (Mic-1) cfa
CANINE:1592139_AT similar to Protein C18orf8 (Colon cancer-associated protein Mic1) (Mic-1) cfa
CANINE_2:CFAAFFX.27860.1.S1_AT similar to Protein C18orf8 (Colon cancer-associated protein Mic1) (Mic-1) cfa
DROSOPHILA_2:1628204_AT dm
DROSGENOME1:148282_AT dm
CHICKEN:GGA.8079.1.S1_AT similar to Chromosome 18 open reading frame 8 gga
CHICKEN:GGA.8079.3.A1_AT similar to Chromosome 18 open reading frame 8 gga
CHICKEN:GGA.8079.3.A1_S_AT similar to Chromosome 18 open reading frame 8 gga
HG-U133_PLUS_2:221190_S_AT chromosome 18 open reading frame 8 hs
HG-U133A:221190_S_AT chromosome 18 open reading frame 8 hs
HG-FOCUS:221190_S_AT chromosome 18 open reading frame 8 hs
HG-U133A_2:221190_S_AT chromosome 18 open reading frame 8 hs
U133_X3P:G7019454_3P_A_AT chromosome 18 open reading frame 8 hs
HG-U133_PLUS_2:232345_AT Chromosome 18 open reading frame 8 hs
HG-U133B:232345_AT Chromosome 18 open reading frame 8 hs
U133_X3P:HS.287633.0.S1_3P_X_AT Chromosome 18 open reading frame 8 hs
U133_X3P:HS.287633.0.S1_3P_AT Chromosome 18 open reading frame 8 hs
HG-U95D:85043_AT Chromosome 18 open reading frame 8 hs
HG-U133_PLUS_2:232348_AT Chromosome 18 open reading frame 8 hs
HG-U133B:232348_AT Chromosome 18 open reading frame 8 hs
HG-U133_PLUS_2:238568_S_AT Chromosome 18 open reading frame 8 hs
HG-U133B:238568_S_AT Chromosome 18 open reading frame 8 hs
HG-U133_PLUS_2:238873_AT Chromosome 18 open reading frame 8 hs
HG-U133B:238873_AT Chromosome 18 open reading frame 8 hs
HG-U95C:61592_AT Chromosome 18 open reading frame 8 hs
HG-U95E:83107_AT Chromosome 18 open reading frame 8 hs
HG-U95E:85795_AT Chromosome 18 open reading frame 8 hs
HG-U95E:89343_AT Chromosome 18 open reading frame 8 hs
MOE430A:1456716_S_AT RIKEN cDNA 3110002H16 gene mm
MOUSE430A_2:1456716_S_AT RIKEN cDNA 3110002H16 gene mm
MOUSE430_2:1456716_S_AT RIKEN cDNA 3110002H16 gene mm
MOUSE430_2:1431330_AT RIKEN cDNA 3110002H16 gene mm
MOUSE430A_2:1431330_AT RIKEN cDNA 3110002H16 gene mm
MOE430A:1431330_AT RIKEN cDNA 3110002H16 gene mm
MU11KSUBA:AF003352_S_AT RIKEN cDNA 3110002H16 gene mm
MG-U74AV2:160433_AT RIKEN cDNA 3110002H16 gene mm
MU19KSUBB:TC33879_S_AT RIKEN cDNA 3110002H16 gene, mRNA (cDNA clone MGC:30355 IMAGE:5027035) mm
MU19KSUBC:TC42124_AT RIKEN cDNA 3110002H16 gene, mRNA (cDNA clone MGC:30355 IMAGE:5027035) mm
RG-U34C:RC_AI180249_AT similar to RIKEN cDNA 2400010D15 (predicted) rn
RAE230A:1374047_AT similar to RIKEN cDNA 2400010D15 (predicted) rn
RAT230_2:1374047_AT similar to RIKEN cDNA 2400010D15 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAK68505 Hypothetical protein Y50D4A.4 [Caenorhabditis elegans] ref|NP_503392.2| Y50D4A.4 [Caenorhabditis elegans] 0.0
blast CAE58017 Hypothetical protein CBG01087 [Caenorhabditis briggsae] 0.0
blast AAK68504 Hypothetical protein Y50D4A.3 [Caenorhabditis elegans] ref|NP_503391.1| Y50D4A.3 [Caenorhabditis elegans] 0.0
blast CAH19081 Hypothetical protein C08B6.4b [Caenorhabditis elegans] ref|NP_001023635.1| C08B6.4b [Caenorhabditis elegans] 1.0E-172
blast CAA96587 Hypothetical protein C08B6.4a [Caenorhabditis elegans] ref|NP_001023634.1| C08B6.4a [Caenorhabditis elegans] 1.0E-172
blast XP_848531 PREDICTED: similar to Protein C18orf8 (Colon cancer-associated protein Mic1) (Mic-1) [Canis familiaris] 1.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR009755 EMBL-EBI Colon cancer-associated Mic1-like 2.5E-100
Pfam IPR009755 EMBL-EBI Colon cancer-associated Mic1-like 2.5E-100
Pfam IPR009755 EMBL-EBI Colon cancer-associated Mic1-like 1.4E-91
Pfam IPR000726 EMBL-EBI Glycoside hydrolase, family 19 8.1E-21
Sequence
Target Sequence 
>C. ELEGANS:176099_AT
atgaggaacaacggggcgctgggactcacttcgaaatcgctgaaaaacgataaaataatg
aatgtccgaacaaaggatcgcggggagccttctactgtgaaattttcgcccgatggccgg
ctctgtgcgatgcaacgcaccccaaccaccgcagacgtggtttttttggaaaaaaccgag
acttctctgtgtttggagttaacagtcaacacaaagtcgaaagaaccattgctggcagtt
gaatgggtcactaataatcagctcctcctcgtgaacaaccatagtgccgaattattatac
attaatgaggataaaaagtcgtcgaaattgttaaaagcgacaaatgtcaacgctgcatgg
gcaatttattatgtaaattcgaacaatttgtcaagattttggcggcgccagtggagaaat
tgctttaaaacatgcctatggtaccaaaacgaccaaatatcatggcgcccagtcagctgc
ttctcatcgcaaatggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAGGAACAACGGGGCGCTGGGAC 378 17 13 Antisense
CAAAGGATCGCGGGGAGCCTTCTAC 626 189 83 Antisense
CTGTGAAATTTTCGCCCGATGGCCG 235 241 107 Antisense
TCTGTGCGATGCAACGCACCCCAAC 251 609 134 Antisense
ACCGAGACTTCTCTGTGTTTGGAGT 498 89 187 Antisense
GGGTCACTAATAATCAGCTCCTCCT 486 497 257 Antisense
TCCTCCTCGTGAACAACCATAGTGC 563 591 275 Antisense
GACAAATGTCAACGCTGCATGGGCA 662 363 351 Antisense
TTGTCAAGATTTTGGCGGCGCCAGT 395 707 400 Antisense
CAAATATCATGGCGCCCAGTCAGCT 127 193 466 Antisense
TCAGCTGCTTCTCATCGCAAATGGC 371 625 485 Antisense
Sequence Source Affymetrix Proprietary Database