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[176094_at]

Description Y65B4A.E /REP_DB=WormBase Gene ID /WP=CE25531 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 88      4grp_total > 26      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 47.7
(0.4)
A 		61.3
(1.6)
A 		10
(-3)
A 		77.1
(3)
A 		67
B 5.3
(-3)
A 		14.8
(-1.5)
A 		43.8
(3)
A 		17
(-1.2)
A 		39
C 5.9
(-3)
A 		59.3
(2.8)
A 		35.8
(0.2)
A 		61.2
(3)
A 		55
avr 19.6
(-3) 		45.1
(1.8) 		29.9
(-1.1) 		51.8
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 25.1
(1.2)
A 		6.1
(-2.7)
A 		4.4
(-3)
A 		9.6
(-1.9)
A 		27.1
(1.6)
A 		33.9
(3)
A 		30
B 9.1
(-1.8)
A 		13
(-1.1)
A 		35
(2.7)
A 		2.4
(-3)
A 		21.6
(0.4)
A 		36.6
(3)
A 		34
C 6
(-2.4)
A 		9.1
(-1.8)
A 		2.8
(-3)
A 		4.5
(-2.7)
A 		17.9
(-0.1)
A 		33.7
(3)
A 		31
avr 13.4
(-1.4) 		9.4
(-2.2) 		14.1
(-1.2) 		5.5
(-3) 		22.2
(0.4) 		34.7
(3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 17.4
(3)
A 		13.3
(1.3)
A 		2.6
(-3)
A 		11.3
(0.5)
A 		15
B 1.7
(-3)
A 		3.6
(-1.6)
A 		10.1
(3)
A 		3.6
(-1.6)
A 		8
C 1.9
(-3)
A 		17
(3)
A 		12.5
(1.2)
A 		10.3
(0.3)
A 		15
avr 7
(-3) 		11.3
(3) 		8.4
(-1) 		8.4
(-1) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 4.6
(-0)
A 		2.2
(-2)
A 		1
(-3)
A 		2.4
(-1.8)
A 		8.2
(2.9)
A 		8.3
(3)
A 		7
B 3.2
(-1.8)
A 		4.3
(-1.3)
A 		8.5
(0.6)
A 		0.6
(-3)
A 		8.8
(0.8)
A 		13.7
(3)
A 		13
C 2.2
(-2.2)
A 		2.8
(-1.8)
A 		0.7
(-3)
A 		0.9
(-2.9)
A 		5.8
(-0.1)
A 		11.3
(3)
A 		11
avr 3.3
(-1.8) 		3.1
(-1.9) 		3.4
(-1.7) 		1.3
(-3) 		7.6
(0.9) 		11.1
(3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176094_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18876
Sequence Type Exemplar sequence
Representative Public ID Y65B4A.E NCBI
Target Description Y65B4A.E /REP_DB=WormBase Gene ID /WP=CE25531 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058360
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058360 NCBI Caenorhabditis elegans Y65B4A.6 (Y65B4A.6) mRNA, complete cds. None
GENEFINDER00000032547 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:630636:636337:-1 None
SNAP00000032535 ENSEMBL cdna:SNAP chromosome:CEL140:I:631097:637935:-1 None
Y65B4A.6 ENSEMBL cdna:known chromosome:CEL140:I:631097:636353:-1 gene:Y65B4A.6 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:631096-636337(-) 81.07 98.36
Public Domain and Genome References
Gene Title
Ensembl Y65B4A.6
Entrez Gene 190485 Entrez gene
SwissProt Q9BL61 EMBL-EBI
WormBase CE34419 Wormbase
Functional Annotations
Ortholog
ID Title Organism
MU11KSUBB:MSA.30713.0_F_AT similar to Probable ATP-dependent helicase DDX48 (DEAD-box protein 48) (Eukaryotic initiation factor 4A-like NUK-34) (Nuclear matrix protein 265) (hNMP 265) (Eukaryotic translation initiation factor 4A isoform 3) mm
MU11KSUBB:MSA.10669.0_F_AT similar to Probable ATP-dependent helicase DDX48 (DEAD-box protein 48) (Eukaryotic initiation factor 4A-like NUK-34) (Nuclear matrix protein 265) (hNMP 265) (Eukaryotic translation initiation factor 4A isoform 3) mm
MG-U74BV2:164744_AT similar to Probable ATP-dependent helicase DDX48 (DEAD-box protein 48) (Eukaryotic initiation factor 4A-like NUK-34) (Nuclear matrix protein 265) (hNMP 265) (Eukaryotic translation initiation factor 4A isoform 3) mm
MG-U74CV2:170484_AT similar to Probable ATP-dependent helicase DDX48 (DEAD-box protein 48) (Eukaryotic initiation factor 4A-like NUK-34) (Nuclear matrix protein 265) (hNMP 265) (Eukaryotic translation initiation factor 4A isoform 3) mm
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29954 Hypothetical protein Y65B4A.6 [Caenorhabditis elegans] ref|NP_490761.2| Y65B4A.6 [Caenorhabditis elegans] 0.0
blast CAE61310 Hypothetical protein CBG05145 [Caenorhabditis briggsae] 0.0
blast AAB96704 Hypothetical protein F33D11.10 [Caenorhabditis elegans] ref|NP_491703.1| F33D11.10 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.8E-37
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 5.0E-79
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.8E-37
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 5.0E-79
Sequence
Target Sequence 
>C. ELEGANS:176094_AT
tttagagctggaaccactcgtgtcctcatttctactgatgtttgggcgagaggacttgat
gttccgcaagtgtccttggtacattttcgattttccgcgaaaaatagcattttcccactg
caactttttcctcacaagggacgaggaaaagtggtttctaggccatggccgaggggccga
caagtttcagcggccatttatcttgctttgttttccgcctgttttctttcgtttttcaca
gctttttcccgttttttctcattaaaactgataaataaatattttttgcagatgctaaaa
caatttccaagtaaaaaaattatgtattcagtcggcaagcagcggcgacaatcgtatttc
ttagtcaaaattctgcaaaaaggtggaatttttcatagaaaatatgtaatcaactacgat
ttgccgaacaaccgtgagctctacatccatcgtatcggccgttcgggacgtttcggccgc
aagggagtcgccatcaatttcgtcaaacaggacgacgtacgaattctccgcgacattgag
cagtact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTAGAGCTGGAACCACTCGTGTCC 294 671 943 Antisense
TCGTGTCCTCATTTCTACTGATGTT 570 605 960 Antisense
GGGCGAGAGGACTTGATGTTCCGCA 275 493 986 Antisense
GATGTTCCGCAAGTGTCCTTGGTAC 626 409 1000 Antisense
TTGGTACATTTTCGATTTTCCGCGA 112 709 1018 Antisense
AATAGCATTTTCCCACTGCAACTTT 694 171 1045 Antisense
AGCAGCGGCGACAATCGTATTTCTT 629 77 1280 Antisense
AACTACGATTTGCCGAACAACCGTG 492 139 1354 Antisense
GGAGTCGCCATCAATTTCGTCAAAC 444 517 1426 Antisense
CAGGACGACGTACGAATTCTCCGCG 353 207 1450 Antisense
ATTCTCCGCGACATTGAGCAGTACT 345 11 1465 Antisense
Sequence Source Affymetrix Proprietary Database