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[176093_at]

Description ZK652.8 /REP_DB=WormBase Gene ID /WP=CE00453 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 80      6grp_total > 20      4grp_total > 60      6grp_high1_sub > 02_03_04_05      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 138.6
(3)
P 		116.2
(2)
A 		102.9
(1.4)
A 		8.4
(-3)
A 		130
B 128.7
(3)
P 		115.2
(2.3)
A 		105.6
(1.8)
A 		11.3
(-3)
A 		117
C 91.2
(0.3)
P 		97
(0.9)
A 		117.2
(3)
P 		59
(-3)
A 		58
avr 119.5
(3) 		109.5
(2.4) 		108.6
(2.3) 		26.2
(-3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 111
1 2 3 4 5 6 range
A 96.7
(-3)
P 		180.4
(3)
P 		143.7
(0.4)
P 		172.2
(2.4)
P 		149.3
(0.8)
A 		105.9
(-2.3)
P 		84
B 80.2
(-2.9)
A 		157.9
(1.3)
P 		188.7
(3)
P 		120.6
(-0.7)
P 		180.5
(2.6)
P 		77.5
(-3)
A 		111
C 164
(1.9)
P 		174.3
(2.6)
P 		136.3
(0.1)
A 		180.3
(3)
P 		160.1
(1.7)
P 		89.2
(-3)
A 		91
avr 113.6
(-1.3) 		170.9
(3) 		156.2
(1.9) 		157.7
(2) 		163.3
(2.4) 		90.9
(-3) 		80
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 50.5
(3)
P 		25.2
(-0.1)
A 		26.9
(0.1)
A 		1.2
(-3)
A 		49
B 41
(3)
P 		28.4
(1)
A 		24.5
(0.4)
A 		2.4
(-3)
A 		39
C 30.2
(0.9)
P 		27.9
(0.5)
A 		40.9
(3)
P 		9.9
(-3)
A 		31
avr 40.6
(3) 		27.2
(0.8) 		30.8
(1.4) 		4.5
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 17.8
(-3)
P 		64.1
(3)
P 		31.3
(-1.3)
P 		43.7
(0.4)
P 		45.1
(0.5)
A 		25.8
(-2)
P 		46
B 27.9
(-3)
A 		52.5
(0.3)
P 		45.8
(-0.6)
P 		31.9
(-2.5)
P 		73.3
(3)
P 		28.9
(-2.9)
A 		45
C 60.8
(3)
P 		53.2
(1.5)
P 		34.7
(-2.1)
A 		36.3
(-1.8)
P 		51.8
(1.3)
P 		29.9
(-3)
A 		31
avr 35.5
(-1.5) 		56.6
(3) 		37.3
(-1.1) 		37.3
(-1.1) 		56.7
(3) 		28.2
(-3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176093_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7362
Sequence Type Exemplar sequence
Representative Public ID ZK652.8 NCBI
Target Description ZK652.8 /REP_DB=WormBase Gene ID /WP=CE00453 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066300(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066300 NCBI Caenorhabditis elegans ZK652.8 (ZK652.8) mRNA, complete cds. 10/11 None
GENEFINDER00000029642 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7842280:7843558:-1 10/11 None
ZK652.8 ENSEMBL cdna:known chromosome:CEL140:III:7842303:7843558:-1 gene:ZK652.8 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000029625 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7842277-7843556(-) 93.16 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK652.8
Entrez Gene 191377 Entrez gene
SwissProt P34665 EMBL-EBI
WormBase CE29164 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA28217 Hypothetical protein ZK652.8 [Caenorhabditis elegans] ref|NP_498701.1| ZK652.8 [Caenorhabditis elegans] sp|P34665|YOY8_CAEEL Hypothetical protein ZK652.8 in chromosome III 5.0E-97
blast CAE66879 Hypothetical protein CBG12258 [Caenorhabditis briggsae] 7.0E-78
blast AAA28217 Hypothetical protein ZK652.8 [Caenorhabditis elegans] ref|NP_498701.1| ZK652.8 [Caenorhabditis elegans] sp|P34665|YOY8_CAEEL Hypothetical protein ZK652.8 in chromosome III 6.0E-75
blast CAE66879 Hypothetical protein CBG12258 [Caenorhabditis briggsae] 4.0E-69
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000029642 1 149-171
Sequence
Target Sequence 
>C. ELEGANS:176093_AT
ggacaaatcacggaattttcagccaaacgagggcgaagaggaaatgaaaaaaagctgggt
acgccacgagaaactgtgtgctcaactcgaggcttgctccttggacctgaaacagaacga
ggcacagatgacaattttgacggctaccggtggagagttgaaggaaaagcacaagaagat
tcgagggcttttggatgaagcaaaggcaaatggtcaacaaaaagaacttgaagagttgtc
aaaggaaattgaaaacgtggaaacacagaccaggatttggctcaatgagcttcacgatgt
acacgataagcgagttgatattgattgccaaatgattcgattaggatctgaagtgaagaa
aaacgagacctatgttcaattggcgtgcattgatatcgaaagaatggagcttcgtcatga
aatcaggccagaaatctacgagatcttctctttctttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACAAATCACGGAATTTTCAGCCA 25 527 66 Antisense
GGGTACGCCACGAGAAACTGTGTGC 237 497 122 Antisense
AACTGTGTGCTCAACTCGAGGCTTG 294 143 137 Antisense
GCTTGCTCCTTGGACCTGAAACAGA 490 305 157 Antisense
GATGACAATTTTGACGGCTACCGGT 652 411 192 Antisense
GACGGCTACCGGTGGAGAGTTGAAG 688 371 204 Antisense
AACACAGACCAGGATTTGGCTCAAT 197 135 327 Antisense
GGCTCAATGAGCTTCACGATGTACA 340 539 344 Antisense
GAGACCTATGTTCAATTGGCGTGCA 533 393 430 Antisense
GGAGCTTCGTCATGAAATCAGGCCA 4 521 471 Antisense
ATCTACGAGATCTTCTCTTTCTTTT 89 33 499 Antisense
Sequence Source Affymetrix Proprietary Database