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[176089_at]

Description Y5H2B.3 /REP_DB=WormBase Gene ID /WP=CE21320 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 57      4grp_total > 27      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 34.3
(2)
A 		40.6
(3)
A 		1.9
(-3)
A 		34.6
(2.1)
A 		39
B 1.4
(-3)
A 		51.4
(2.8)
A 		7.6
(-2.3)
A 		53.5
(3)
A 		52
C 2.1
(-2.9)
A 		38.2
(2.5)
A 		1.2
(-3)
A 		41.9
(3)
A 		41
avr 12.6
(-1.6) 		43.4
(3) 		3.6
(-3) 		43.3
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 39.6
(1)
A 		3.3
(-2.9)
A 		38.7
(0.9)
A 		21.6
(-0.9)
A 		2.4
(-3)
A 		58.3
(3)
A 		56
B 26.6
(0.8)
A 		10.4
(-1.6)
A 		35.3
(2.1)
A 		4.4
(-2.5)
A 		1.3
(-3)
A 		41.3
(3)
A 		40
C 15.9
(-1.2)
A 		5.8
(-2.4)
A 		1.4
(-3)
A 		50.9
(3)
A 		1.2
(-3)
A 		39.4
(1.6)
A 		50
avr 27.4
(0.5) 		6.5
(-2.3) 		25.1
(0.2) 		25.6
(0.2) 		1.6
(-3) 		46.3
(3) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 12.5
(3)
A 		8.8
(1.2)
A 		0.5
(-3)
A 		5.1
(-0.7)
A 		12
B 0.5
(-3)
A 		12.7
(3)
A 		1.8
(-2.4)
A 		11.4
(2.4)
A 		12
C 0.7
(-2.8)
A 		11
(3)
A 		0.4
(-3)
A 		7.1
(0.8)
A 		11
avr 4.6
(-0.8) 		10.8
(3) 		0.9
(-3) 		7.9
(1.2) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 7.3
(-0.1)
A 		1.2
(-2.8)
A 		8.4
(0.4)
A 		5.5
(-0.9)
A 		0.7
(-3)
A 		14.2
(3)
A 		14
B 9.3
(0.5)
A 		3.5
(-1.8)
A 		8.6
(0.3)
A 		1.2
(-2.7)
A 		0.5
(-3)
A 		15.4
(3)
A 		15
C 5.9
(-0.4)
A 		1.8
(-2.3)
A 		0.4
(-3)
A 		10.2
(1.6)
A 		0.4
(-3)
A 		13.2
(3)
A 		13
avr 7.5
(0) 		2.2
(-2.3) 		5.8
(-0.7) 		5.6
(-0.8) 		0.5
(-3) 		14.3
(3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176089_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15431
Sequence Type Exemplar sequence
Representative Public ID Y5H2B.3 NCBI
Target Description Y5H2B.3 /REP_DB=WormBase Gene ID /WP=CE21320 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071194(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071194 NCBI Caenorhabditis elegans Y5H2B.3 (Y5H2B.3) mRNA, complete cds. 11/11 None
SNAP00000028125 ENSEMBL cdna:SNAP chromosome:CEL140:V:2188123:2190041:1 10/11 None
GENEFINDER00000028137 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2188123:2188671:1 11/11 None
Y5H2B.3 ENSEMBL cdna:known chromosome:CEL140:V:2188123:2188671:1 gene:Y5H2B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2188122-2188671(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y5H2B.3
Entrez Gene 189362 Entrez gene
SwissProt Q9N4Q9 EMBL-EBI
WormBase CE21320 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503595 Y5H2B.3 [Caenorhabditis elegans] gb|AAF59633.1| Hypothetical protein Y5H2B.3 [Caenorhabditis elegans] 4.0E-47
blast NP_503595 Y5H2B.3 [Caenorhabditis elegans] gb|AAF59633.1| Hypothetical protein Y5H2B.3 [Caenorhabditis elegans] 2.0E-39
blast AAC25878 Hypothetical protein C45H4.14a [Caenorhabditis elegans] ref|NP_503586.1| C45H4.14a [Caenorhabditis elegans] 2.0E-15
Sequence
Target Sequence 
>C. ELEGANS:176089_AT
acggcaaatacctcaaagacctcatggcaaagattcttcgcggagaagagagcatcaatt
ccgcagatgcagtagaaatcctgcacattcctttgaaacttgctctggagatcggagtta
tcgactctatggagggtctcgatccatacggagaaatgaagatttcatgcaagcggattt
tgaaaaagcttgagcgaaagaagaaggctttggctaatcaaaaagttacggaccctgcca
agaaggaacacaaggaacacgagcaagaagcaaaagatctcatcaagaaggagaagtctg
agcagaagaaggaagccaaggagcaagtgaataaggaaaagtctgaagagaagaaaaagg
aatccgtaaagagaaagtcatcggaaaaattggaggcaccatccaagaaagccaagaatt
ctgaggatcaagaaaatcaagtagccatcaaaaagaaggtcactccaccgaagaccaagc
ataccgccctcttcaaaaagtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGCAAATACCTCAAAGACCTCAT 522 95 26 Antisense
GACCTCATGGCAAAGATTCTTCGCG 527 383 43 Antisense
GAGAAGAGAGCATCAATTCCGCAGA 578 391 68 Antisense
CAGTAGAAATCCTGCACATTCCTTT 443 205 95 Antisense
CACATTCCTTTGAAACTTGCTCTGG 40 197 109 Antisense
GTTATCGACTCTATGGAGGGTCTCG 179 447 142 Antisense
GGAGGGTCTCGATCCATACGGAGAA 178 505 156 Antisense
TCAAAAAGTTACGGACCCTGCCAAG 554 599 243 Antisense
GGAGGCACCATCCAAGAAAGCCAAG 486 515 417 Antisense
CTCCACCGAAGACCAAGCATACCGC 47 237 488 Antisense
GCATACCGCCCTCTTCAAAAAGTGC 500 299 504 Antisense
Sequence Source Affymetrix Proprietary Database